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Cytotoxic Activity of Black Tea Theaflavin Digallates Against Chinese Hamster Ovary Cells (CHOK1) and Rat Glioma Cells (C-6)

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Chemistry of Natural Compounds Aims and scope

The present study reports the comparative in vitro cytotoxicity of theaflavin digallate (2) and isotheaflavin digallate (1), a new black tea theaflavin against Chinese hamster ovary cells (CHOK1) and rat glioma cells (C-6). Compound 1 was formed by the immobilized polyphenol oxidase (PPO) mediated enzymatic oxidation of (–) GCG and (–) ECG along with compound 2, which was formed by enzymatic oxidation of (–) EGCG and (–) ECG. The structures of the compounds were elucidated using ESI-Q-TOF-MS and 1D and 2D NMR. The results of a comparative cytotoxicity assay have revealed that isotheaflavin digallate is more cytotoxic than theaflavin digallate (IC50 246.8 μg/mL against CHOK1, IC50 288.8 μg/mL against C-6 cells).

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Acknowledgment

We express our gratitude to the Director, CSIR-Institute of Himalayan Bioresource Technology for encouragement.

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Authors and Affiliations

  1. Hill Area Tea Science Division, Palampur, India

    Ajay Rana, Robin Joshi, Ashu Gulati & Harsh Pratap Singh

  2. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India

    Dharmesh Kumar

Authors
  1. Ajay Rana
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  2. Dharmesh Kumar
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  3. Robin Joshi
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  4. Ashu Gulati
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  5. Harsh Pratap Singh
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Corresponding author

Correspondence to Ashu Gulati.

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Published in Khimiya Prirodnykh Soedinenii, No. 5, September–October, 2015, pp. 718–721.

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Rana, A., Kumar, D., Joshi, R. et al. Cytotoxic Activity of Black Tea Theaflavin Digallates Against Chinese Hamster Ovary Cells (CHOK1) and Rat Glioma Cells (C-6). Chem Nat Compd 51, 835–839 (2015). https://doi.org/10.1007/s10600-015-1428-z

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  • DOI: https://doi.org/10.1007/s10600-015-1428-z

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