Peptides containing six and eight cysteines were isolated by acid extraction and subsequent three-step chromatography from Nigella sativa sprouts. The molecular weights of the resulting fractions were determined by MALDI-TOF mass spectrometry.
Similar content being viewed by others
References
P. C. Selitrennikoff, Appl. Environ. Microbiol., 67, 2883 (2001).
W. F. Broekaert, B. P. A. Cammue, M. F. C. De Bolle, K. Thevissen, G. W. De Samblanx, and R. W. Osborn, Crit. Rev. Plant Sci., 16, 297 (1997).
F. Garcia-Olmedo, A. Molina, J. M. Alamillo, and P. Rodriguez-Palenzuela, Biopolymers, 47, 479 (1998).
F. Garcia-Olmedo, P. Rodriguez-Palenzuela, A. Molina, J. M. Alamillo, E. Lopez-Salanilla, M. Berrocal-Lobo, and C. Poza-Carrion, FEBS Lett., 498, 219 (2001).
Yu. I. Oshchepkova, E. A. Rogozhin, O. N. Veshkurova, Ts. A. Egorov, and Sh. I. Salikhov, Khim. Prir. Soedin., 247 (2009).
Yu. I. Oshchepkova, O. N. Veshkurova, E. A. Rogozhin, A. Kh. Musolyamov, A. N. Smirnov, T. I. Odintsova, Ts. A. Egorov, E. V. Grishin, and Sh. I. Salikhov, Bioorg. Khim., 35, 344 (2009).
Acknowledgment
The work was supported financially by The Coordinating Committee for Development of Science and Technology of the Republic of Uzbekistan Cabinet of Ministers (Grant FA-F3-T140) and the RFBR (Grant 08-04-90257).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 580–581, September–October, 2009.
Rights and permissions
About this article
Cite this article
Oshchepkova, Y.I., Rogozhin, E.A., Veshkurova, O.N. et al. Isolation of cysteine-rich peptides from Nigella sativa sprouts. Chem Nat Compd 45, 690–692 (2009). https://doi.org/10.1007/s10600-009-9432-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10600-009-9432-9