A peptide fraction was extracted from chickpea sprouts using 50% EtOH. The peptides were further purified and analyzed by LC-MS. Their molecular masses (MM) were determined using HPLC over a C18 reversedphase column. Six pure peptides that differed from those isolated previously from chickpeas were obtained. The peptides had various antimicrobial activities against Candida albicans (CA), Escherichia coli (EC), and Staphylococcus aureus (SA).
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Acknowledgment
The research was financially supported by The Light of the Western Talent Training Plan, Project No. LHXZ-2014-01, and Research and Development of Key Technology for High Quality Expanded Chickpea Products, No. 201491160, of the Key Technology and Application Demonstration of the Production and Value-Added Processing of Chick Pea, No. 2015BAD29B03-4. We also thank the Central Asia Drug Research and Development Center of the Chinese Academy of Sciences for support of the research.
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Translated from Khimiya Prirodnykh Soedinenii, No. 6, November–December, 2018, pp. 965–967.
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Waili, A., Gao, Y.H., Zhang, L.F. et al. Production of Antimicrobial Peptides from Cicer arietinum Sprouts and Determination of Their Molecular Masses. Chem Nat Compd 54, 1135–1138 (2018). https://doi.org/10.1007/s10600-018-2573-y
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DOI: https://doi.org/10.1007/s10600-018-2573-y