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Inbreeding depression from selfing and mating between relatives in the Neotropical tree Cariniana legalis Mart. Kuntze

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Abstract

Selfing or mating between related individuals in self-compatible hermaphroditic tree species may lead to inbreeding depression (ID) due to homozygosis in recessive, identical by descent alleles. In general, studies of ID in tree species have been based on comparisons of selfed individuals (produced by controlled pollination) with outcrossed individuals for quantitative traits in progeny tests. However, this approach requires a long time to quantify the extent of ID. Thus, we used an approach based on genetic markers to estimate coancestry coefficients between assigned parents from paternity analysis in two populations of the Neotropical tree Cariniana legalis. Using this method, we were able to determine which seedlings in a nursery trial originated from; (i) outcrossing between un-related trees, (ii) mating between related trees and (iii) selfing. We detected a low selfing rate (<10 %), but a substantial quantity of seedlings from mating between related parents (minimum of 35.7 %). In general, the outcrossed seedlings from unrelated parents exhibited significantly greater genetic diversity than those resulting from selfing and mating among relatives. The extent of ID varied among traits and populations. Outcrossed seedlings originating from unrelated trees generally showed greater survival than seedlings originating from selfing and related parents. Inbreeding depression was greater in the selfed seedlings than in those from mating among related parents. The results are discussed in terms of implications for genetic conservation, breeding and environmental restoration using the species.

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Acknowledgments

We thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP - 2010/10704-7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq - 470491/2010-8) for funding this project. Evandro V. Tambarussi was supported by a FAPESP PhD scholarship (grant 2010/12354-3). Alexandre M. Sebbenn, Miguel L.M. Freitas and Roland Vencovsky are supported by CNPq research fellowships. We thank Tiago Gabassi for help in the laboratory and Wladimir Correa and Dirceu de Souza for their help in collecting the plant samples. We also thank NPGLE for corrections to the English.

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Authors and Affiliations

  1. Universidade Estadual do Centro-Oeste, PR 153, Km 7, Irati, PR, 84500-000, Brazil

    Evandro Vagner Tambarussi

  2. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK

    David Boshier

  3. Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Av. Pádua Dias, 11, Caixa Postal 9, Piracicaba, SP, 13418-900, Brazil

    Roland Vencovsky

  4. Instituto Florestal de São Paulo, CP 1322, São Paulo, SP, 01059-970, Brazil

    Miguel Luiz Menezes Freitas & Alexandre Magno Sebbenn

Authors
  1. Evandro Vagner Tambarussi
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  2. David Boshier
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  3. Roland Vencovsky
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  4. Miguel Luiz Menezes Freitas
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  5. Alexandre Magno Sebbenn
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Correspondence to Evandro Vagner Tambarussi.

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Tambarussi, E.V., Boshier, D., Vencovsky, R. et al. Inbreeding depression from selfing and mating between relatives in the Neotropical tree Cariniana legalis Mart. Kuntze. Conserv Genet 18, 225–234 (2017). https://doi.org/10.1007/s10592-016-0896-4

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  • DOI: https://doi.org/10.1007/s10592-016-0896-4

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