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Crossing the Rhine: a potential barrier to wildcat (Felis silvestris silvestris) movement?

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Abstract

The European wildcat (Felis silvestris silvestris) underwent a severe decline across Europe in the early twentieth century. Remaining populations are often very small and isolated, though there are indications that wildcat populations are currently expanding their range. However, linear landscape elements such as rivers and roads are thought to present barriers to dispersal, inhibiting gene flow and, thus, affecting the recolonization process. In this study, we investigated the fine-scale genetic structure of wildcats in the Upper Rhine Valley. We specifically analysed wildcats on both sides of the Rhine River by genotyping 55 individual wildcats, using 20 microsatellite loci. Genetic differentiation was weak and positive spatial autocorrelation was found up to a distance of 10 km (females: 5 km, males: 10 km) indicating substantial gene flow among sampling sites. High levels of gene flow, even across the Rhine River, indicated that the water body itself does not necessarily have a strong barrier effect, which is in contrast to other studies. Our findings could best be explained by the populations’ history, a local extinction east of the River Rhine and a current ongoing population expansion. Our study highlights that potential barriers, such as rivers, may have different effects in different local wildcat populations and that the history of the populations is important to interpret genetic results. As many wildcats still occur in isolated and patchy forest fragments, maintaining connectivity between populations is crucial to ensure their viability in the long term.

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References

  • Balkenhol N, Waits LP (2009) Molecular road ecology: exploring the potential of genetics for investigating transportation impacts on wildlife. Mol Ecol 18:4151–4164. doi:10.1111/j.1365-294X.2009.04322.x

    Article  PubMed  Google Scholar 

  • Birlenbach K, Klar N (2009) Aktionsplan zum Schutz der Europäischen Wildkatze in Deutschland. Naturschutz Landsch 41:325–332

    Google Scholar 

  • Biró Z, Szemethy L, Heltai M (2004) Home range sizes of wildcats (Felis silvestris) and feral domestic cats (Felis silvestris f. catus) in a hilly region of Hungary. Mamm Biol—Z Säugetierkd 69:302–310. doi:10.1078/1616-5047-00149

    Article  Google Scholar 

  • Blanco JC, Cortés Y, Virgós E (2005) Wolf response to two kinds of barriers in an agricultural habitat in Spain. Can J Zool 83:312–323

    Article  Google Scholar 

  • Brooks TM, Mittermeier RA, Mittermeier CG et al (2002) Habitat loss and extinction in the hotspots of biodiversity. Conserv Biol 16:909–923

    Article  Google Scholar 

  • Chen C, Durand E, Forbes F, François O (2007) Bayesian clustering algorithms ascertaining spatial population structure: A new computer program and a comparison study. Mol Ecol Notes 7:747–756

    Article  Google Scholar 

  • Cozzi G, Broekhuis F (2013) Comparison of the effects of artificial and natural barriers on large African carnivores: implications for interspecific relationships and connectivity. J Anim Ecol 82:707–715

    Article  PubMed  Google Scholar 

  • Devictor V, Julliard R, Jiguet F (2008) Distribution of specialist and generalist species along spatial gradients of habitat disturbance and fragmentation. Oikos. doi:10.1111/j.2008.0030-1299.16215.x

    Google Scholar 

  • Earl DA, VonHoldt BM (2011) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the evanno method. Conserv Genet Resour 4:359–361. doi:10.1007/s12686-011-9548-7

    Article  Google Scholar 

  • Eckert I (2003) DNA-Analysen zum genetischen Status der Wildkatze (Felis silvestris) in Deutschland. 1–101

  • Eckert I, Suchentrunk F, Markov G, Hartl GB (2010) Genetic diversity and integrity of German wildcat (Felis silvestris) populations as revealed by microsatellites, allozymes, and mitochondrial DNA sequences. Mamm Biol—Z Säugetierkd 75:160–174. doi:10.1016/j.mambio.2009.07.005

    Article  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620. doi:10.1111/j.1365-294X.2005.02553.x

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  Google Scholar 

  • Goossens B, Waits LP, Taberlet P (1998) Plucked hair samples as a source of DNA: reliability of dinucleotide microsatellite genotyping. Mol Ecol 7:1237–1241

    Article  CAS  PubMed  Google Scholar 

  • Greenwood PJ (1980) Mating systems, philopatry and dispersal in birds and mammals. Anim Behav 28:1140–1162

    Article  Google Scholar 

  • Harestad AS, Bunnell FL (1979) Home range and body weight—a reevaluation. Ecology 60:389–402

    Article  Google Scholar 

  • Hartmann SA, Steyer K, Kraus RHS et al (2013) Potential barriers to gene flow in the endangered European wildcat (Felis silvestris). Conserv Genet 14:413–426. doi:10.1007/s10592-013-0468-9

    Article  Google Scholar 

  • Hepenstrick D, Thiel D, Holderegger R, Gugerli F (2012) Genetic discontinuities in roe deer (Capreolus capreolus) coincide with fenced transportation infrastructure. Basic Appl Ecol 13:631–638. doi:10.1016/j.baae.2012.08.009

    Article  Google Scholar 

  • Herdtfelder M, Strein M, Suchant R (2007) Wildkatzen am Kaiserstuhl. Naturschutz Landsch 10:320–328

    Google Scholar 

  • Herrmann M, Vogel C (2005) Wildkatze Felis silvestris sivestris Schreber, 1777. In: Braun M, Dieterlen F (eds) Die Säugetiere Baden-Württembergs, vol 2. Eugen Ulmer, Stuttgart, pp 363–376

    Google Scholar 

  • Hertwig ST, Schweizer M, Stepanow S et al (2009) Regionally high rates of hybridization and introgression in German wildcat populations (Felis silvestris, Carnivora, Felidae). J Zool Syst Evol Res 47:283–297

    Article  Google Scholar 

  • Holderegger R, Di Giulio M (2010) The genetic effects of roads: a review of empirical evidence. Basic Appl Ecol 11:522–531. doi:10.1016/j.baae.2010.06.006

    Article  Google Scholar 

  • Jakobsson M, Rosenberg N (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806. doi:10.1093/bioinformatics/btm233

    Article  CAS  PubMed  Google Scholar 

  • Jombart T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24(11):1403–1405

    Article  CAS  PubMed  Google Scholar 

  • Jombart T, Devillard S, Balloux F (2010) Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genetics 11(1):1

    Article  Google Scholar 

  • Klar N, Herrmann M, Kramer-Schadt S (2009) Effects and mitigation of road impacts on individual movement behavior of wildcats. J Wildl Manag 73:631–638. doi:10.2193/2007-574

    Article  Google Scholar 

  • McKelvey KS, Schwartz MK (2005) Dropout: a program to identify problem loci and samples for noninvasive genetic samples in a capture-mark-recapture framework. Mol Ecol Notes 5:716–718. doi:10.1111/j.1471-8286.2005.01038.x

    Article  CAS  Google Scholar 

  • Menotti-Raymond M, David VA, Lyons LA et al (1999) A genetic linkage map of microsatellites in the domestic cat (Felis catus). Genomics 57:9–23. doi:10.1006/geno.1999.5743

    Article  CAS  PubMed  Google Scholar 

  • Monterroso P, Brito JC, Ferreras P, Alves PC (2009) Spatial ecology of the European wildcat in a Mediterranean ecosystem: dealing with small radio-tracking datasets in species conservation. J Zool 279:27–35. doi:10.1111/j.1469-7998.2009.00585.x

    Article  Google Scholar 

  • Müller U, Strein M, Suchant R (2003) Wildtierkorridore in Baden-Württemberg. Berichte Freiburger forstliche Forschung 48:46

    Google Scholar 

  • Navidi W, Arnheim N, Waterman MS (1992) A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations. Am J Hum Genet 50:347–359

    CAS  PubMed  PubMed Central  Google Scholar 

  • Noss RF, Quigley HB, Hornocker MG et al (1996) Conservation biology and carnivore conservation in the Rocky mountains. Conserv Biol 10:949–963

    Article  Google Scholar 

  • Nowell K, Jackson P (1996) Wild cats. status survey and conservation action plan. IUCN/SSC Cat Specialist Group, Switzerland, pp 1–383

    Google Scholar 

  • Nussberger B, Greminger MP, Grossen C et al (2013) Development of SNP markers identifying European wildcats, domestic cats, and their admixed progeny. Mol Ecol Resour 13:447–460. doi:10.1111/1755-0998.12075

    Article  CAS  PubMed  Google Scholar 

  • Oliveira R, Godinho R, Randi E et al (2007) Molecular analysis of hybridisation between wild and domestic cats (Felis silvestris) in Portugal: implications for conservation. Conserv Genet 9:1–11. doi:10.1007/s10592-007-9297-z

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetics software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in excel. Population genetic software for teaching and research–an update. Bioinformatics 28:2537–2539. doi:10.1093/bioinformatics/bts460

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Piechoki R (1990) Die Wildkatze - Felis silvestris., Neue Brehm BüchereiA Ziemsen, Wittenberg Lutherstadt

    Google Scholar 

  • Pierpaoli M, Biro ZS, Herrmann M et al (2003) Genetic distinction of wildcat (Felis silvestris) populations in Europe, and hybridization with domestic cats in Hungary. Mol Ecol 12:2585–2598. doi:10.1046/j.1365-294X.2003.01939.x

    Article  CAS  PubMed  Google Scholar 

  • Piry S, Luikart G, Cornuet JM (1999) BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. J Hered 90:502–503

    Article  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed  PubMed Central  Google Scholar 

  • Raimer F (1988) Die Wildkatze in Hessen und Niedersachsen - Biotop, Umwelt, Verbreitung, Bestandsentwicklung, Gefährdung, Schutz

  • R Development Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.r-project.org/

  • Revilla E, Wiegand T, Palomares F et al (2004) Effects of matrix heterogeneity on animal dispersal: from individual behavior to metapopulation-level parameters. Am Nat 164:E130–E153. doi:10.1086/424767

    Article  PubMed  Google Scholar 

  • Rosenberg NA (2003) Distruct: a program for the graphical display of population structure. Mol Ecol Notes 4:137–138. doi:10.1046/j.1471-8286.2003.00566.x

    Article  Google Scholar 

  • Say L, Devillard S, Léger F et al (2012) Distribution and spatial genetic structure of European wildcat in France. Anim Conserv 15:18–27. doi:10.1111/j.1469-1795.2011.00478.x

    Article  Google Scholar 

  • Stahl P, Artois M (1991) Status and conservation of the wildcat in Europe and around the Mediterranean rim. Council of Europe, Strasbourg

    Google Scholar 

  • Stahl P, Artois M, Aubert MFA (1988) Organisation spatiale et déplacement des chats forestiers adultes (Felis silvestris Schreber 1777) en Lorraine. Rev d’Ecologie, Terre Vie 43:113–132

    Google Scholar 

  • Steyer K, Simon O, Kraus RHS et al (2012) Hair trapping with valerian-treated lure sticks as a tool for genetic wildcat monitoring in low-density habitats. Eur J Wildl Res 59:39–46. doi:10.1007/s10344-012-0644-0

    Article  Google Scholar 

  • Streif S, Kraft S, Veith S et al (2012) Monitoring and research of the European wildcat (Felis silvestris) in Baden-Württemberg. Säugetierkundliche Informationen 8:411–416

    Google Scholar 

  • Taberlet P, Waits L, Luikart G (1999) Noninvasive genetic sampling: look before you leap. Trends Ecol Evol 14:323–327

    Article  PubMed  Google Scholar 

  • Thiel C (2004) Streifgebiete und Schwerpunkte der Raumnutzung von Felis silvestris silvestris (Schreber 1777) in der Nordeifel. 1–195

  • Thompson DJ, Jenks JA (2010) Dispersal movements of subadult cougars from the Black Hills: the notions of range expansion and recolonization. Ecosphere. doi:10.1890/ES10-00028.1

    Google Scholar 

  • Vallinoto M, Araripe J, Rego PS et al (2006) Tocantins river as an effective barrier to gene flow in Saguinus niger populations. Genet Mol Biol 29:215–219

    Article  Google Scholar 

  • Wilcove DS, McLellan CH, Dobson AP (1986) Habitat fragmentation in the temperate zone. In: Soule ME (ed) Conservation biology: the Science of Scarcity and Diversity. Sinauer Associates, Sunderland, pp 237–256

    Google Scholar 

  • Woodroffe R, Ginsberg J (1998) Edge effects and the extinction of populations inside protected areas. Science 280:2126–2128

    Article  CAS  PubMed  Google Scholar 

  • Wright S (1943) Isolation by distance. Genetics 28(2):114

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

We are grateful to the numerous persons who helped to conduct this research, in particular, our collegues at the Forest Research Institute Baden Wuerttemberg, Stéphanie Kraft, Sarah Veith, Mara Sandrini and Rudi Suchant for their support and constructive discussions. Sampled wildcat material and expertise was provided by François Léger (Office National de la Chasse et de la Faune Sauvage), Beatrice Nussberger (Institute of Evolutionary Biology and Environmental Studies, University of Zurich), Mathias Herrmann (ÖKO-LOG), Kathrin Steyer (Conservation Genetics, Senckenberg Research Institution and Natural History Museum Frankfurt), the Zentrum für Fisch- und Wildtiermedizin (Vetsuisse-Faculty, University of Bern) and the numerous people who regularly controlled our lure sticks. We acknowledge the funding for this study from the Landesjagdabgabe Baden Wuerttemberg. Stephanie Witczak helped revising the English of the present paper. We thank the two anonymous referees for providing constructive comments and help in improving the contents of this study.

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Authors and Affiliations

  1. Forest Research Institute of Baden-Wuerttemberg FVA, Wonnhaldestr. 4, 79100, Freiburg im Breisgau, Germany

    Sandra Würstlin, Sabrina Streif & Annette Kohnen

  2. Wildlife Ecology and Management, University Freiburg, Tennenbacher Straße 4, 79106, Freiburg, Germany

    Gernot Segelbacher

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  1. Sandra Würstlin
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Correspondence to Sandra Würstlin.

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Würstlin, S., Segelbacher, G., Streif, S. et al. Crossing the Rhine: a potential barrier to wildcat (Felis silvestris silvestris) movement?. Conserv Genet 17, 1435–1444 (2016). https://doi.org/10.1007/s10592-016-0874-x

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