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Ten microsatellite loci from Northern Bobwhite (Colinus virginianus)

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Abstract

Ecological studies using microsatellite data often require the selection of an optimal marker set for use in parentage and relatedness inference. Commonly, this requires a candidate pool of microsatellite markers from which several are selected to ensure data are acquired efficiently and accurately. We developed 10 microsatellite loci for use with Northern Bobwhite (Colinus virginianus) and tested these loci using individuals collected from two distinct populations in GA and VA. Our new markers yielded seven alleles/locus (range: 2–16) in the Georgia population and six alleles/locus (range: 2–13) in the Virginia population. Exclusionary power of all markers in each population with both parents unknown was >0.98. These microsatellite loci should be combined with previously developed markers to select an optimal set for use in subsequent analyses of parentage and relatedness.

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Acknowledgments

Financial support was provided by: Tall Timbers Research Station and Land Conservancy’s Quail Research Initiative, Daniel B. Warnell School of Forestry and Natural Resources, and McIntire-Stennis projects GEO-0100-MS and GEO-0136-MS. Capture and handling of bobwhites in Georgia were approved by the Georgia Department of Natural Resources (permits: 29-WMB-00–105 and 29-WMB-03–38), and the University of Georgia Institutional Animal Care and Use Committee (approvals: A990028M1, A990028C1, and A200310109-0). Bobwhite wings collected in Virginia were salvaged from hunter-killed animals as part of a Virginia Department of Game and Inland Fisheries program. The authors thank Patrick Cook and Steve Capel for the donation of these wings.

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Authors and Affiliations

  1. D. B. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA

    Brant C. Faircloth, Theron M. Terhune & John P. Carroll

  2. Tall Timbers Research Station and Land Conservancy, 13093 Henry Beadel Drive, Tallahassee, FL, 32312, USA

    Brant C. Faircloth & William E. Palmer

  3. Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC, 29802, USA

    Nancy A. Schable & Travis C. Glenn

  4. Environmental Health Science, College of Public Health, University of Georgia, Athens, GA, 30602, USA

    Travis C. Glenn

  5. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, 90095, USA

    Brant C. Faircloth

Authors
  1. Brant C. Faircloth
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  2. Theron M. Terhune
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  3. Nancy A. Schable
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  4. Travis C. Glenn
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  5. William E. Palmer
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  6. John P. Carroll
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Corresponding author

Correspondence to Brant C. Faircloth.

Additional information

This note has been submitted to Conservation Genetics and no other journal. We presented details regarding creation of the enriched library described in this manuscript in Schable et al. (2004). The primers described herein were developed in another laboratory and are independent of development efforts outlined in Schable et al. (2004), other than library construction. We have retained authors where appropriate to recognize their library development efforts. In this manuscript, we use updated sequencing and primer design techniques and different sets of individuals for polymorphism screening.

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Faircloth, B.C., Terhune, T.M., Schable, N.A. et al. Ten microsatellite loci from Northern Bobwhite (Colinus virginianus). Conserv Genet 10, 535–538 (2009). https://doi.org/10.1007/s10592-008-9559-4

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  • DOI: https://doi.org/10.1007/s10592-008-9559-4

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