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Development of single sequence repeat markers for the ant Aphaenogaster senilis and cross-species amplification in A. iberica, A . gibbosa, A. subterranea and Messor maroccanus

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Abstract

Eight polymorphic microsatellite loci were isolated and characterized for Aphaenogaster senilis, a common ant species distributed in the Western Mediterranean. Characterization of 15 individuals form southern Spain showed moderate to high allelic diversity ranging from 2 to 9 alleles per locus. Cross-species tests on 10 individuals of A. iberica, A. gibbosa, A. subterranea and Messor maroccanus revealed successful amplification for most loci. This set of markers can be useful for population genetic studies and might even prove useful in other phylogenetically close species of the subfamily Myrmicinae.

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Acknowledgements

The authors would like to thank Ana Carvajal and Isabel Luque for their valuable help in extracting DNA from samples as well as Alain Lenoir for providing A. subterranea. This project was funded by grant CGL2006-04968/BOS for JAG, RB and XC and the French grant ANR-06-BLAN-0268 CD, PF, HM and TM.

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Correspondence to J. A. Galarza.

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Galarza, J.A., Boulay, R., Cerdá, X. et al. Development of single sequence repeat markers for the ant Aphaenogaster senilis and cross-species amplification in A. iberica, A . gibbosa, A. subterranea and Messor maroccanus . Conserv Genet 10, 519–521 (2009). https://doi.org/10.1007/s10592-008-9554-9

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  • DOI: https://doi.org/10.1007/s10592-008-9554-9

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