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Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients

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Abstract

Background CyclinD1 and p16 are involved in the regulation of G1 checkpoint and may play an important role in the tumorgenesis of laryngeal squamous cell carcinoma (LSCC). Previous studies have examined the level of expression of cyclinD1 or p16 in LSCC but no such information is available for their relation and their correlation with lymph node metastasis in Chinese patients. Patients and methods A total of 58 patients underwent surgical resection of laryngeal tumours in the Department of Otolaryngology, Qilu Hospital Shandong University between January 2001 and December 2002. All pathologic specimens were available for immunohistochemical study using antibodies against cyclinD1 and p16. Results Compared with normal epithelium, expression of CyclinD1 in the LSCC was significantly higher (62.1% vs. 10.0%, P < 0.05), expression of p16 was significantly lower (48.3% vs. 90.0%, P < 0.05); CyclinD1 expression in LSCC was up-regulated in TNM classification (r s = 0.409, P < 0.05) as well as with cervical lymph node metastases (r s = 0.294, P < 0.05); p16 expression in LSCC was down-regulated with cervical lymph node metastases (r s = −0.275, P < 0.05); negative significant correlation between p16 immunostaining and CyclinD1 was observed in LSCC (r s = −0.331, P < 0.05). Conclusion There was a negative correlation between CyclinD1 expression and p16 expression in LSCC. The over-expression of CyclinD1 and the under-expression of p16 may play a significant role in the incidence and development of LSCC and may be important indicators for cervical lymph node metastases in Chinese patients of LSCC.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Shandong Provincial Qianfoshan Hospital, Clinical Medical College of Shandong University, Jinan, China

    Zhi-jie Fu & Qi-rong Wang

  2. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University, Jinan, China

    Zhi-yong Ma, Rong Wang & Chun-xi Liu

  3. Department of Otorhinolaryngology, QiLu Hospital, Shandong University, West Wenhua Road #107, Jinan, 250012, People’s Republic of China

    Da-peng Lei & Xin-liang Pan

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  1. Zhi-jie Fu
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  2. Zhi-yong Ma
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  3. Qi-rong Wang
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  4. Da-peng Lei
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  5. Rong Wang
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  6. Chun-xi Liu
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  7. Xin-liang Pan
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Correspondence to Xin-liang Pan.

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Zhi-jie Fu and Zhi-yong Ma are contributed to this work equally.

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Fu, Zj., Ma, Zy., Wang, Qr. et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients. Clin Exp Metastasis 25, 887–892 (2008). https://doi.org/10.1007/s10585-008-9207-x

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  • DOI: https://doi.org/10.1007/s10585-008-9207-x

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