Abstract
Purpose: The urokinase plasminogen activator (uPA) and its receptor (uPAR) are expressed by pancreatic cancer cells and can be targeted by the plasminogen activator inhibitor type 2 (PAI2). We have labeled PAI2 with 213Bi to form the alpha conjugate (AC), and have studied its in vitro cytotoxicity and in vivo efficacy.
Methods and Materials: The expression of uPA/uPAR on pancreatic cell lines, human pancreatic cancer tissues, lymph node metastases, and mouse xenografts were detected by immunohistochemistry, confocal microscopy, and flow cytometry. Cytotoxicity was assessed by the MTS and TUNEL assay. At 2 days post-cancer cell subcutaneous inoculation, mice were injected with AC by local or systemic injection.
Results: uPA/uPAR is strongly expressed on pancreatic cancer cell lines and cancer tissues. The AC can target and kill cancer cells in vitro in a concentration-dependent fashion. Some 90% of TUNEL positive cells were found after incubation with 1.2 MBq/ml of AC. A single local injection of ~222 MBq/kg 2 days post-cell inoculation can completely inhibit tumor growth over 12 weeks, and an intraperitoneal injection of 111 MBq/kg causes significant tumor growth delay.
Conclusions: 213Bi-PAI2 can specifically target pancreatic cancer cells in vitro and inhibit tumor growth in vivo. 213Bi-PAI2 may be a useful agent for the treatment of post-surgical pancreatic cancer patients with minimum residual disease.
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References
SF Sener A Fremgen HR Menck et al. (1999) ArticleTitlePancreatic cancer: a report of treatment and survival trends for 100,313 patients diagnosed from 1985–1995, using the National Cancer Database J Am Coll Surgeons 189 1–7 Occurrence Handle1:STN:280:DyaK1MzivVWlsg%3D%3D
M Yamamoto O Ohashi Y Saitoh (1998) ArticleTitleJapan Pancreatic Cancer Registry: current status Pancreas 16 238–42 Occurrence Handle10.1097/00006676-199804000-00006 Occurrence Handle1:STN:280:DyaK1c3gvVCnsA%3D%3D Occurrence Handle9548661
KC Conlon DS Klimstra MF Brennan (1996) ArticleTitleLong-term survival after curative resection for pancreatic ductal adenocarcinoma. Clinicopathologic analysis of 5-year survivors Ann Surg 223 273–279 Occurrence Handle1:STN:280:BymC1Mbgtlc%3D Occurrence Handle8604907
DM Goldenberg (2002) ArticleTitleTargeted therapy of cancer with radiolabeled antibodies J Nucl Med 43 693–713 Occurrence Handle1:CAS:528:DC%2BD38XktlOlur0%3D Occurrence Handle11994535
TM Behr B Wormann M Gramatzki et al. (1999) ArticleTitleLow- versus high-dose radioimmunotherapy with humanized anti-CD22 or chimeric anti-CD20 antibodies in a broad spectrum of B cell-associated malignancies Clin Cancer Res 5 3304s–14s Occurrence Handle1:CAS:528:DyaK1MXnsVShtLc%3D Occurrence Handle10541379
DM Goldenberg (2001) ArticleTitleThe role of radiolabeled antibodies in the treatment of non-Hodgkin’s lymphoma: the coming of age of radioimmunotherapy Crit Rev Oncol Hematol 39 195–201 Occurrence Handle1:STN:280:DC%2BD38%2Fit1ahsQ%3D%3D Occurrence Handle11418316
DV Gold DE Modrak K Schutsky et al. (2004) ArticleTitleCombined 90Yttrium-DOTA-labeled PAM4 antibody radioimmunotherapy and gemcitabine radiosensitization for the treatment of a human pancreatic cancer xenograft Int J Cancer 109 618–26 Occurrence Handle10.1002/ijc.20004 Occurrence Handle1:CAS:528:DC%2BD2cXislCis7s%3D Occurrence Handle14991585
MS Kaminski J Estes KR Zasadny et al. (2000) ArticleTitleRadioimmunotherapy with iodine (131)I tositumomab for relapsed or refractory B-cell non-Hodgkin lymphoma: updated results and long-term follow-up of the University of Michigan experience Blood 96 1259–66 Occurrence Handle1:CAS:528:DC%2BD3cXlvF2it7k%3D Occurrence Handle10942366
MR McDevitt G Sgouros RD Finn et al. (1998) ArticleTitleRadioimmunotherapy with alpha-emitting nuclides Eur J Nucl Med 25 1341–51 Occurrence Handle10.1007/s002590050306 Occurrence Handle1:CAS:528:DyaK1cXlsV2lu7s%3D Occurrence Handle9724387
BJ Allen (1999) ArticleTitleCan alpha-immunotherapy succeed where other systemic modalities have failed Nucl Med Commun 20 205–7 Occurrence Handle1:STN:280:DyaK1M7ptlShug%3D%3D Occurrence Handle10093068
SJ Kennel R Boll M Stabin et al. (1999) ArticleTitleRadioimmunotherapy of micrometastases in lung with vascular targeted 213Bi Br J Cancer 80 175–84 Occurrence Handle1:CAS:528:DyaK1MXjtFyksb8%3D Occurrence Handle10389994
B Allen N Blagojevic (1997) ArticleTitleAlpha and beta emitting radiolanthanides in targeted cancer therapy Nucl Med Commun 20 205–7
Y Li SM Rizvi M Ranson et al. (2002) ArticleTitle213Bi-PAI2 conjugate selectively induces apoptosis in PC3 metastatic prostate cancer cell line and shows anti-cancer activity in a xenograft animal model Br J Cancer 86 1197–203 Occurrence Handle10.1038/sj.bjc.6600179 Occurrence Handle1:CAS:528:DC%2BD38XjvFGisbs%3D Occurrence Handle11953871
AM Ballangrud WH Yang S Palm et al. (2004) ArticleTitleAlpha-particle emitting atomic generator (Actinium-225)-labeled trastuzumab (herceptin) targeting of breast cancer spheroids: efficacy versus HER2/neu expression Clin Cancer Res 10 4489–97 Occurrence Handle1:CAS:528:DC%2BD2cXlsVWlurw%3D Occurrence Handle15240541
C Seidl H Schrock S Seidenschwang et al. (2005) ArticleTitleCell death triggered by alpha-emitting 213Bi-immunoconjugates in HSC45-M2 gastric cancer cells is different from apoptotic cell death Eur J Nucl Med Mol Imag 32 274–85 Occurrence Handle1:CAS:528:DC%2BD2MXis1ajt7Y%3D
GJ Beyer M Miederer S Vranjes-Duric et al. (2004) ArticleTitleTargeted alpha therapy in vivo: direct evidence for single cancer cell kill using 149Tb-rituximab Eur J Nucl Med Mol Imag 31 547–54 Occurrence Handle1:CAS:528:DC%2BD2cXitFygsLo%3D
JG Jurcic SM Larson G Sgouros et al. (2002) ArticleTitleTargeted alpha particle immunotherapy for myeloid leukemia Blood 100 1233–9 Occurrence Handle1:CAS:528:DC%2BD38Xmt12itL8%3D Occurrence Handle12149203
M Schmitt OG Wilhelm U Reuning et al. (2000) ArticleTitleThe urokinase plasminogen activation system as a novel target for tumour therapy Fibrinol Proteol 14 114–32 Occurrence Handle10.1054/fipr.2000.0079 Occurrence Handle1:CAS:528:DC%2BD3cXksVKht7s%3D
J Pollanen RW Stephens A Vaheri (1991) ArticleTitleDirected plasminogen activation at the surface of normal and malignant cells Adv Cancer Res 57 273–328 Occurrence Handle1:CAS:528:DyaK38XhslWit7w%3D Occurrence Handle1950706
PA Andreasen R Egelund HH Petersen (2000) ArticleTitleThe plasminogen activation system in tumor growth, invasion, and metastasis Cell Mol Life Sci 57 25–40 Occurrence Handle1:CAS:528:DC%2BD3cXhslOrsrk%3D Occurrence Handle10949579
BM Mueller YB Yu WE Laug (1995) ArticleTitleOverexpression of plasminogen activator inhibitor 2 in human melanoma cells inhibits spontaneous metastasis in scid/scid mice Proc Nat Acad Sci U.S.A. 92 205–9 Occurrence Handle1:CAS:528:DyaK2MXjtVegu7Y%3D
K Marutsuka Y Hasui Y Asada et al. (1995) ArticleTitleEffects of suramin on metastatic ability, proliferation, and production of urokinase-type plasminogen activator and plasminogen activator inhibitor type 2 in human renal cell carcinoma cell line SN12C-PM6 Clin Exp Metastasis 13 116–22 Occurrence Handle10.1007/BF00133616 Occurrence Handle1:CAS:528:DyaK2MXlsFamt7Y%3D Occurrence Handle7882614
EK Kruithof MS Baker CL Bunn (1995) ArticleTitleBiological and clinical aspects of plasminogen activator inhibitor type 2 Blood 86 4007–24 Occurrence Handle1:CAS:528:DyaK2MXpslakurk%3D Occurrence Handle7492756
MS Baker P Bleakley GC Woodrow et al. (1990) ArticleTitleInhibition of cancer cell urokinase plasminogen activator by its specific inhibitor PAI-2 and subsequent effects on extracellular matrix degradation Canc Res 50 4676–84 Occurrence Handle1:CAS:528:DyaK3cXkvFGmsLs%3D
F Al-Ejeh D Croucher M Ranson (2004) ArticleTitleKinetic analysis of plasminogen activator inhibitor type-2: urokinase complex formation and subsequent internalisation by carcinoma cell lines Exp Cell Res 297 259–71 Occurrence Handle1:CAS:528:DC%2BD2cXkvVKmtr8%3D Occurrence Handle15194441
C Apostolidis R Carlos-Marquez W Janssens et al. (2001) ArticleTitleCancer treatment using Bi-213 and Ac-225 in radioimmunotherapy Nucl News 44 29–33
M Ranson Z Tian NM Andronicos et al. (2002) ArticleTitleIn vitro cytotoxicity of bismuth-213 (213Bi)-labeled-plasminogen activator inhibitor type 2 (alpha-PAI-2) on human breast cancer cells Breast Cancer Res Treat 71 149–59 Occurrence Handle10.1023/A:1013850107791 Occurrence Handle1:CAS:528:DC%2BD38XitFehsLo%3D Occurrence Handle11881911
Y Gavrieli Y Sherman SA Ben-Sasson (1992) ArticleTitleIdentification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation J Cell Biol 119 493–501 Occurrence Handle10.1083/jcb.119.3.493 Occurrence Handle1:CAS:528:DyaK38XmtVyhsbs%3D Occurrence Handle1400587
M Gleave JT Hsieh CA Gao et al. (1991) ArticleTitleAcceleration of human prostate cancer growth in vivo by factors produced by prostate and bone fibroblasts Cancer Res 51 3753–61 Occurrence Handle1:STN:280:By6B1M3kvVA%3D Occurrence Handle1712249
BJ Allen Z Tian SM Rizvi et al. (2003) ArticleTitlePreclinical studies of targeted alpha therapy for breast cancer using 213Bi-labelled-plasminogen activator inhibitor type 2 Br J Cancer 88 944–50 Occurrence Handle10.1038/sj.bjc.6600838 Occurrence Handle1:CAS:528:DC%2BD3sXitFegtrk%3D Occurrence Handle12644835
W Wang JL Abbruzzese D Evans et al. (1999) ArticleTitleOverexpression of urokinase-type plasminogen activator in pancreatic adenocarcinoma is regulated by constitutively activated RelA Oncogene 18 4554–63 Occurrence Handle1:CAS:528:DyaK1MXlslCrtbc%3D Occurrence Handle10467400
D Cantero H Friess J Deflorin et al. (1997) ArticleTitleEnhanced expression of urokinase plasminogen activator and its receptor in pancreatic carcinoma Br J Cancer 75 388–95 Occurrence Handle1:STN:280:ByiC2s3ktVQ%3D Occurrence Handle9020484
Y Takeuchi A Nakao A Harada et al. (1993) ArticleTitleExpression of plasminogen activators and their inhibitors in human pancreatic carcinoma: immunohistochemical study Am J Gastroenterol 88 1928–33 Occurrence Handle1:CAS:528:DyaK2cXhvVynt74%3D Occurrence Handle8237943
RM Macklis JY Lin B Beresford et al. (1992) ArticleTitleCellular kinetics, dosimetry, and radiobiology of alpha-particle radioimmunotherapy: induction of apoptosis Radiation Research 130 220–6 Occurrence Handle1:CAS:528:DyaK38XisFWiurs%3D Occurrence Handle1574578
F Al-Ejeh D Croucher M Ranson (2004) ArticleTitleKinetic analysis of plasminogen activator inhibitor type-2: urokinase complex formation and subsequent internalsiation by carcinoma cell lines. Exp Cell Res 297 259–71 Occurrence Handle1:CAS:528:DC%2BD2cXkvVKmtr8%3D Occurrence Handle15194441
M Harms-Ringdahl P Nicotera IR Radford (1996) ArticleTitleRadiation induced apoptosis Mutat Res 366 171–9 Occurrence Handle1:CAS:528:DyaK28Xnt1Oit7k%3D Occurrence Handle9001584
JF Ward (1994) ArticleTitleThe complexity of DNA damage: relevance to biological consequences Int J Radiat Biol 66 427–32 Occurrence Handle1:CAS:528:DyaK2MXisFCkurw%3D Occurrence Handle7983426
Errol C Friedberg Graham C Walker W Siede (1995) DNA Repair and Mutagenesis ASM Press Washington D.C
BJ Allen C Raja SMA Rizvi et al. (2004) ArticleTitleTargeted alpha therapy for cancer Phys Med Biol 49 3703–12 Occurrence Handle1:CAS:528:DC%2BD2cXnvVCqtL4%3D Occurrence Handle15446799
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Qu, C.F., Song, E.Y., Li, Y. et al. Pre-clinical Study of 213Bi Labeled PAI2 for the Control of Micrometastatic Pancreatic Cancer. Clin Exp Metastasis 22, 575–586 (2005). https://doi.org/10.1007/s10585-005-5788-9
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DOI: https://doi.org/10.1007/s10585-005-5788-9