Abstract
The endogenous methylated derivative of ʟ-arginine, Nω,Nω′-dimethyl-ʟ-arginine (asymmetric dimethylarginine, ADMA), an independent risk factor in many diseases, inhibits the activity of nitric oxide synthases and, consequently, modulates the availability of nitric oxide. While most studies on the biological role of ADMA have focused on endothelial and inducible nitric oxide synthases modulation and its contribution to cardiovascular, metabolic, and renal diseases, a role in regulating neuronal nitric oxide synthases and pathologies of the central nervous system is less understood. The two isoforms of dimethylarginine dimethylaminohydrolase (DDAH), DDAH1 and DDAH2, are thought to be the main enzymes responsible for ADMA catabolism. A current impediment is limited knowledge on specific tissue and cellular distribution of DDAH enzymes within the brain. In this study, we provide a detailed characterization of the regional and cellular distribution of DDAH1 and DDAH2 proteins in the adult murine and human brain. Immunohistochemical analysis showed a wide distribution of DDAH1, mapping to multiple cell types, while DDAH2 was detected in a limited number of brain regions and exclusively in neurons. Our results provide key information for the investigation of the pathophysiological roles of the ADMA/DDAH system in neuropsychiatric diseases and pave the way for the development of novel selective therapeutic approaches.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Dimethylarginine dimethylaminohydrolases (DDAH) are a family of enzymes that metabolize methylated arginines. In mammals, there are two isoforms, DDAH1 and DDAH2, which have around 50% identity in their amino acid sequences (Frey et al. 2006) and differential tissue expression patterns (Leiper et al. 1999; Dayal et al. 2008). DDAH1 mRNA is widely expressed in mammals, particularly in the kidney, brain, and liver, while DDAH2 mRNA is primarily found in the heart, lung, and placenta (Dayal et al. 2008; Leiper et al. 1999). DDAH1 was first discovered by Ogawa and colleagues, who also identified that its catabolic reaction results in the formation of citrulline and dimethylamine (Ogawa et al. 1987). There are four forms of methylated arginine, which are formed ubiquitously through degradation of proteins (Kakimoto and Akazawa 1970; McDermott 1976), namely Nω-monomethyl-ʟ-arginine (ʟ-NMMA), Nω,Nω′-dimethyl-ʟ-arginine (asymmetric dimethylarginine, ADMA), Nω,Nω-dimethyl-ʟ-arginine (symmetric dimethylarginine, SDMA), and N-δ-methylarginine, which is found in yeast and human cells (Niewmierzycka and Clarke 1999; Zobel-Thropp et al. 1998; Martens-Lobenhoffer et al. 2016). However, in mammals, only ʟ-NMMA and ADMA are primarily hydrolyzed by the DDAH enzymes, while SDMA is mainly removed by renal excretion (Ogawa et al. 1989; McDermott 1976; Leiper et al. 1999).
Research on DDAH has mostly focused on its role in metabolizing ADMA, given that plasma concentrations of ADMA are higher than those of ʟ-NMMA (Meyer et al. 1997). Moreover, ADMA is linked to various pathologies; with elevated ADMA concentration observed in endothelial dysfunction, chronic renal failure, hypertension, heart failure, diabetes, and atherosclerosis (Boger 2005; Zoccali et al. 2001; Usui et al. 1998; Miyazaki et al. 1999; Leone et al. 1992; Vallance et al. 1992; Chen et al. 2012). ADMA is a potent inhibitor of nitric oxide synthases (NOSs), a family of enzymes responsible for the production of nitric oxide (NO). Thus, DDAH inhibition leads to increased ADMA concentrations and, consequently, reduced NO availability (MacAllister et al. 1996). Conversely, overexpression of DDAH1 results in reduced ADMA concentration and increased NO production (Ayling et al. 2006). Furthermore, ADMA causes uncoupling of NOS, a process that leads to the production of superoxides (Toth et al. 2007; Antoniades et al. 2009).
NO is a gaseous signaling molecule that is involved in various biological processes, including the modulation of central nervous system (CNS) functions (Calabrese et al. 2007). There are three variants of the NOS enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS). Within the CNS, nNOS and eNOS have been shown to regulate learning and memory formation, neurogenesis, and long-term synaptic transmission, while iNOS has an immunoregulatory function (Asif et al. 2013; Chen et al. 2005; Böhme et al. 1993; Hölscher et al. 1996; Son et al. 1996; Zhou et al. 2007; Sonar and Lal 2019). Upregulation of nNOS and/or iNOS has been implicated in brain ischemia and the pathogenesis of neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease (Zhou et al. 2007; Gatto et al. 2000; Izumi et al. 1992; Gang Zhang et al. 1994; Hannemann et al. 2020). Numerous studies have also demonstrated the key role of the nNOS-NO pathway in the etiology of affective disorders such as depression (Selley 2004; Baranyi et al. 2015; Ozden et al. 2020) and bipolar affective disorder (Sağlam Aykut et al. 2012; Ustundag et al. 2020). This suggests that the modulation of NOS activity may have therapeutic effects also in CNS disease states (reviewed in Dhir and Kulkarni 2011; Freudenberg et al. 2015). As ADMA is the major endogenous inhibitor of NOS, targeting DDAH may represent a promising strategy for the development of new treatment approaches.
The function of DDAH isoforms in the brain however, remains elusive. Assessing the pathophysiological role of DDAH in the brain requires in-depth knowledge of regional and cellular DDAH expression profiles to inform how modulation of DDAH will affect different brain regions or cellular subtypes. We sought to address this issue by providing detailed mapping of DDAH1 and DDAH2 regional and cellular protein expression in the murine brain and comparative analysis in human post-mortem tissue samples.
Materials and Methods
Animals and Tissue Collection
Experiments were conducted in ten male C57Bl/6 J mice 10–12 weeks old purchased from the Jackson Laboratory, USA. Animals were housed in a 12-h light–dark cycle (lights on at 06:00) with food and water ad libitum. All efforts were made to reduce animal suffering and the number of animals used. Mice were deeply anesthetized with a mixture of 100 mg/kg ketamine and 10 mg/kg xylazine, transcardially perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA). Brains were dissected and post-fixed in 4% PFA overnight, cryoprotected in 20% sucrose in PBS for up to 4 days, frozen in methylbutane at − 40 °C and then stored at − 80 °C. Coronal or sagittal sections of 40 µm thickness were cut on a freezing microtome (Leica CM1850) and stored in antifreeze (25% glycerol, 25% ethylene glycol in PBS) at − 20 °C until further processing.
Human Tissue Collection
Frontal gyrus samples from normal human brain tissue were obtained through the National Health and Medical Research Council South Australian Brain Bank. Brain samples were extracted and embedded in paraffin. Sample blocks were cut in 8 µm sections and mounted onto gelatin-coated slides. Additional brain tissue samples were obtained from a 49-year-old man diagnosed with acute lymphoblastic leukemia obtained from N.N. Petrov National Medical Research Center of Oncology, Russia. Brain samples were extracted and dissected according to The Human Brain Atlas into the prefrontal cortex and amygdala (The Human Brain 2020). Samples were post-fixed in 10% PFA for seven days and then cryoprotected in 15% sucrose in PBS for up to 4 days. Sections of 35 µm thickness were cut on a freezing microtome (Leica CM1850UV) and placed directly on glass slides.
Immunohistochemical Staining
Brain sections were deparaffinized by incubation in xylene followed by washing with decreasing ethanol concentrations (100%, 95%, 75%) for 5 min each. All further washing steps were performed in tris-buffered saline (TBS) with azide (TBS with 0.1% sodium azide). For elimination of endogenous peroxidase activity, slides were covered with 1% H2O2–50% methanol and placed in a humidified chamber for 10 min. Then, sections were washed and blocked in 20% normal horse serum (NHS) in TBS-azide for 1 h. Slides were incubated overnight with primary antibody solution (1% NHS in TBS-azide). All antibodies and respective dilutions used are listed in Table 1. Sections were washed 3 × 5 min and incubated with biotin-conjugated secondary antibody solution (1% NHS in TBS-azide). Sections were further washed 3 × 5 min and incubated with ABC-kit solution (Vector PK-4000, Vectastain) for 60 min. Sections were again washed 3 × 5 min and exposed to the DAB reaction (D4293, SigmaFast, dissolved in 5 ml of TBS and 3.5 µl H2O2). The reaction was stopped with TBS-azide. After brief washing, sections were counter-stained with hematoxylin, dehydrated (three washes in 100% ethanol, two washes in xylene) and mounted with DPX (mixture of distyrene, a plasticizer, and xylene).
Immunofluorescence
Immunofluorescence staining on mouse samples was carried out on free-floating sections. For human samples, antigen retrieval was performed by boiling the slides in citrate buffer (0.01 M, pH 6.0) for 10 min. Initial washing was performed 3 × 15 min in PBS followed by blocking in 10% normal donkey serum (NDS, ab7475, Abcam) and 0.2% Triton X-100 in PBS for 2 h at room temperature. Sections were then incubated overnight at 4 °C in primary antibody solution (3% NDS and 0.2% Triton X-100 in PBS). Sections were washed 3 × 15 min with PBS and incubated with fluorescence conjugated secondary antibody solution (3% NDS and 0.2% Triton X-100 in PBS) for 2 h at room temperature. All antibodies tested are listed in Supplementary Tables S1 and S2, while the antibodies used for the final study, including the dilutions, are listed in Table 1. Sections were then washed 3 × 15 min in PBS and nuclei were counterstained for 10 min with 4′,6-diamidino-2-phenylindole (DAPI) in PBS. After a final 10 min wash, sections were mounted on glass slides (SuperFrost Ultra Plus, Thermo Scientific) with Mowiol (MERCK, #475,904). For double staining, sections were incubated in mixed primary antibody solutions. Samples were visualized and analyzed using Zeiss Observer Z.1 (ApoTome II) widefield microscopy or Zeiss LSM880 confocal microscopy, using Zen 3.2 (blue edition) and ImageJ 1.50e software (Schneider et al. 2012).
Results
Comprehensive antibody validation experiments were carried out using western blot and cell and tissue immunostaining. This approach allowed analysis of various available antibodies against DDAH1 and DDAH2 in order to identify the most specific antibodies for the final study (Supplementary Tables S1 and S2, Supplementary Material Figs. S1 and S2). We focused on the molecular mass of the protein band and its intensity in addition to the specificity of antibody binding with endogenous DDAH1 and DDAH2.
DDAH1 Distribution in the Mouse Brain
To investigate DDAH1 protein distribution in the brain, we initially used anti-DDAH1 staining on mouse brain sections. As shown in Fig. 1a, DDAH1 was widely distributed with an intense signal in the striatum, cortex and hippocampal formation (HPF). A moderate signal was observed in the thalamus and cerebellum (CB). In the striatal regions, cortex, HPF and CB the DDAH1 positive cells had an area of 12–16 µm2, star-shaped soma and many processes. In addition, a few cells of the same size but with a round soma and low expression level of DDAH1 were observed. Moreover, DDAH1 positive cells with star-shaped morphology were detected in white matter structures such as corpus callosum. In the thalamus and in some structures of the midbrain and hindbrain, low levels of DDAH1 expression were observed in round-shaped cells with 16–18 µm2 area, which was slightly bigger than the cells in the HPF and striatum.
Within cortical regions, DDAH1 immunoreactivity was intense in layers I–IV and isocortex (Fig. 1a, box 1). In the striatum, cells with DDAH1 expression were evenly distributed. DDAH1 positive cells were detected in both the dorsal and ventral regions, in the lateral septal complex, and, in a small amount, in the striatum-like amygdalar nuclei. The most intense DDAH1 expression, however, was observed in the dorsal region of the striatum (Fig. 1a, box 2). In the thalamus, the DDAH1 signal was moderate and present only in a limited number of brain nuclei such as the paraventricular nucleus (PVT) (Fig. 1a, box 3) and the reticular nucleus (RT). In HPF, few DDAH1 positive cells were present in Ammon’s horn (cornu Ammonis, CA) and the dentate gyrus (DG). In the CA, DDAH1 positive cells were observed in the stratum oriens (so), stratum radialis (sr), and stratum lacunosum-moleculare (slm) layers (Fig. 1a, box 4). In the DG, strong DDAH1 expression was present in the polymorph and molecular layers. Interestingly, no DDAH1 positive cells were found in both the pyramidal layer (sp) of CA and the granule cell layer in the DG. However, we observed some processes of DDAH1 positive cells that were passing through these layers. DDAH1 immunoreactivity was strong in different olfactory areas. High expression levels were observed in the main olfactory bulb (MOB), anterior olfactory nucleus (AON) and in the layer II of the piriform cortex (Fig, 1a, box 5). In CB, DDAH1 immunoreactivity was low in the Purkinje layer, without any detectable DDAH1 expression in other layers and cerebellar nuclei (Fig. 1c). Additionally, some DDAH1 positive cells were observed in the dorsal nucleus Raphe (Fig. 1b).
We compared our findings to publicly available mRNA expression datasets (Allen Institute for Brain Science 2010; Linnarsson Lab 2020), observing similar and intense expression profiles in the cortex, CB and MOB as well as low expression in the hypothalamus. Further high levels of DDAH1 protein in the striatum and HPF mapped to low Ddah1 expression (Allen Institute for Brain Science 2010). On the contrary, low IHC reactivity reflected intense Ddah1 expression in the ventral tegmental area. Notably, the strongest Ddah1 mRNA signals were found in a wide number of thalamic nuclei (Allen Institute for Brain Science 2010), while we observed only moderate DDAH1 protein expression restricted to a small number of nuclei, including the PVT and RT (see Supplementary Table S3).
DDAH1 is Expressed in Both Neuronal and Glial Cells
Next, we determined specific cell type expression by co-labeling analysis of DDAH1 with a neuronal marker (NeuN), astrocyte markers glial fibrillary acidic protein (GFAP) and S100 calcium-binding protein B (S100beta), oligodendrocyte transcription factor (Olig2), ionized calcium-binding adapter molecule 1 (Iba1) and platelet/endothelial cell adhesion molecule 1 (PECAM-1) (Fig. 2h). On the one hand, we found an overlapping signal between the neuronal marker NeuN and DDAH1 in RT and PVT (Fig. 2a) but no overlap between DDAH1 and the astrocyte marker GFAP (Fig. 2d). On the other hand, DDAH1 and GFAP were expressed by the same cells in all layers of the HPF (Fig. 2c), but we did not observe any overlap between DDAH1 and NeuN (Fig. 2b). In cortical and striatal regions, DDAH1 cells did overlap with S100beta (Supplementary Material, Fig. S3). Immunocytochemical analysis on primary cell culture of cortical and hippocampal origin confirmed the presence of DDAH1 in astrocytes and neurons (Supplementary Material, Fig. S4a-c). Additionally, we performed co-staining for DDAH1 and other cell-type markers such as Olig2 for oligodendrocytes, Iba1 for microglia and PECAM-1 for endothelial cells. We observed partial co-labeling between DDAH1 and PECAM-1 throughout the brain, however the majority of PECAM-1 did not overlap with DDAH1 (Fig. 2e). Despite prevalent Iba1 expression, e.g., within the striatum, we did not detect any co-labeling with DDAH1 (Fig. 2f). Furthermore, no co-labeling between DDAH1 and Olig2 was found (Fig. 2g). In summary, we found that DDAH1 is widely distributed in the rodent brain and expressed in a region-specific manner in both neuronal and astrocyte cells as well as within the endothelium of the vascular structures.
DDAH2 Protein is Expressed in a Limited Number of Brain Regions
To build a map of DDAH2 protein expression, we firstly investigated its distribution on consecutive coronal mouse brain sections. As shown in Fig. 3a, DDAH2 immunoreactivity was detected in a limited number of brain structures. We found high expression levels in the cortex, HPF, striatum, and pallidum. Low expression was observed in the cortical subplate. In all structures, DDAH2 positive cells had a round-shaped soma, an area of 15–19 µm2 and stained processes.
Within the striatum, intense DDAH2 staining was observed in the ventral region, the lateral septal complex and the striatum-like amygdalar nuclei but not in the dorsal part. DDAH2 positive cells were observed in the lateral septal nucleus (LSN) (Fig. 3d) of the lateral septal complex. Here, DDAH2 signal was detected in the caudal part of LSN, while no staining was apparent in the rostral and ventral parts. In the ventral striatum, DDAH2 positive cells were found in the fundus of the striatum (FS) (Fig. 3e) and the olfactory tubercle (OT) (Fig. 3b). DDAH2 immunoreactivity was also observed within the nucleus accumbens. In the striatum-like amygdalar nucleus, intense DDAH2 expression was found in the central amygdalar nuclei (CEA) (Fig. 3a, box 2), without staining in other nuclei such as the anterior or medial amygdalar nucleus. In the pallidum, DDAH2 staining was observed in the caudal part in the bed nuclei of the stria terminalis (BST) (Fig. 3c). There was no DDAH2 fluorescence signal in other regions of the pallidum. Within the cortical regions, DDAH2 immunoreactivity was restricted to layer II of the piriform cortex (Fig. 3a, box. 3) and layer II of the entorhinal area. Low DDAH2 expression was detected in the layer II of the auditory area, somatosensory, orbital, prelimbic, infralimbic and posterior parietal association areas in the cortex. Further, we observed region-specific DDAH2 expression in the HPF restricted to the pyramidal layer of CA1 while no signal was observed in CA2 and CA3 (Fig. 3a, box 1). Additionally, intense DDAH2 expression was observed in the subiculum and the presubiculum. Finally, within the cortical subplate, DDAH2 positive cells were restricted to the endopiriform nucleus.
Again, we compared our findings to the publicly available mRNA expression datasets (Allen Institute for Brain Science 2010; Linnarsson Lab 2020). Ddah2 mRNA signals in the cortex, in olfactory areas and in the HPF, matched our protein expression data (refer to Table S4). However, there were differences in Ddah2 mRNA distribution within the sub-regions of the HPF documented by the Allen Institute for Brain Science (Allen Institute for Brain Science 2010) and our data. According to the published data, Ddah2 mRNA can be identified in CA1, CA2 and CA3, whereas Ddah2 protein signal in our study was restricted to CA1. Striatal protein expression was found but could not be compared in detail due to the limited sagittal data availability on the Allen Mouse Brain Atlas (Allen Institute for Brain Science 2010). Finally, Ddah2 mRNA expression in the HPF, striatum, and hypothalamus was confirmed in our protein assessment in accordance with the known Ddah2 mRNA distribution available at the Mouse Brain Atlas (Linnarsson Lab 2020).
DDAH2 is Expressed Exclusively Within Neurons
Based on the already obtained cell size and shape information, we performed a co-labeling analysis with the neuronal marker NeuN. We found a complete overlap of DDAH2 positive cells with NeuN in all structures (Fig. 4). DDAH2/NeuN expression was observed in CA1, while NeuN immunoreactivity continued in CA2 and CA3 (Fig. 4a). The neuronal origin of DDAH2 positive cells was also confirmed for layer II of the piriform cortex, while NeuN positive cells in both layers I and III did not express DDAH2 (Fig. 4b). The CEA showed specific DDAH2 and NeuN positive staining, which was not observed in other amygdalar nuclei (Fig. 4c). Intense DDAH2 and NeuN positive expression was seen in the caudal part of the LSN (Fig. 4d). For validation, we also performed an analysis of DDAH2 and GFAP double staining, which did not yield any co-labeling (Fig. 4e). Similarly, PECAM-1 positive cerebral endothelial cells did not display DDAH2 signal (Fig. 4f), despite strong signal in peripheral vascular beds (Supplementary Material, Fig. S5). Results of the immunocytochemical analysis on early postnatal primary cell culture of cortical and hippocampal origin confirmed the presence of DDAH2 in neurons; however, a minor fraction of DDAH2 positive cells also stained for GFAP (Fig. S4d–f). In summary, within the adult mouse brain, DDAH2 protein was found to be expressed exclusively in neuronal cells within a limited number of brain structures.
DDAH1 and DDAH2 are Expressed by Different Cell Populations
Next, we performed co-labeling experiments of DDAH1 and DDAH2. As shown in Fig. 5, both DDAH1 and DDAH2 signals were observed in the HPF, cortex and striatum. However, DDAH1 and DDAH2 were consistently expressed by different cells. In the HPF, DDAH2 was present only in neurons of sp of CA1, whereas DDAH1 positive cells were observed in astrocytes in other layers (so, sr, slm) (Fig. 5c). In the striatum, DDAH2 was expressed by neurons in a few striatal structures such as LSN, whereas DDAH1 was broadly present in astrocytes (Fig. 5b). Additionally, DDAH2 and DDAH1 expression was observed in the BST (Fig. 5b) and entorhinal cortex but did not show cellular overlap Fig. 5d. In summary, our results suggest that DDAH1 and DDAH2 protein are detected in the same regions of the brain but always in a cell-type restricted manner.
Identical Cell Types in Mouse and Human Brain Tissue Express DDAH1 and DDAH2
Finally, we performed a comparative analysis in human and murine tissue. We established staining of DDAH1 and DDAH2 on human post-mortem tissue to compare its distribution with our murine data (Fig. 6). At first, we performed DAB staining in tissue samples from the medial frontal gyrus, where both DDAH1 (Figs. 6a, b) and DDAH2 (Figs. 6e, f) signals were observed. DDAH1 positive cells appeared small (app. 15 µm2) and star-shaped with multiple processes reminiscent of astrocytes, whilst DDAH2 positive cells had neuronal features and an area of app. 19 µm2. Comparable findings are derived from DDAH1 expression in mouse (Fig. 6d) and human cortex (Fig. 6c) as well as DDAH2 staining on mouse (Fig. 6h) and human (Fig. 6g) amygdala. In summary, similar cell types express DDAH1 and DDAH2 in both human and mouse CNS tissue.
Discussion
The main findings of our study are that: (1) DDAH1 is broadly distributed in the adult mouse brain; (2) DDAH1 is expressed in both neuronal and astrocyte cells as well as within the endothelia of the vascular structures; (3) DDAH2 is expressed exclusively in neuronal cells within a limited number of brain structures; (4) DDAH1 and DDAH2 can be expressed in the same brain region but not within the same cell; (5) The distribution pattern of DDAH1 and DDAH2 in the brain is similar in mice and humans.
Previous studies on murine DDAH1 protein expression have been sparse. In line with our histochemical results, a strong DDAH1 expression was observed in whole brain homogenate (Hu et al. 2011) and regional expression was reported for the hippocampus, dorsal root ganglia and spinal cord (DʼMello et al. 2015). Also, DDAH1 protein was detected in primary cell culture with cortical origin (Dowsett et al. 2015). Further support for our findings comes from databases reporting wide Ddah1 mRNA distribution throughout the mouse brain and for distinct cell types (Linnarsson Lab 2020; Allen Institute for Brain Science 2010). Our findings confirmed the expression of DDAH1 in neurons and astrocytes but not in oligodendrocytes and low expression in endothelial cells. Nevertheless, the regional distribution of DDAH1 protein in the mouse brain is more limited compared to the database mRNA expression profile. The discrepancy may be explained by post-transcriptional and post-translational regulation in addition to differences in stability of both mRNA and protein (de Sousa Abreu et al. 2009). Another technical issue could be the use of antibodies. Although specificity was tested prior to application, sensitivity could be a limiting factor for the detection of low protein expression levels.
Likewise, an early study in human tissue samples from various organs showed strong DDAH1 mRNA expression in the brain (Leiper et al. 1999), which was later confirmed by the Human Protein Atlas and the HGNC database (EMBL-EBI 2020; Human Protein Atlas 2020; Uhlén et al. 2015). Additionally, mRNA dot blot analysis using isoform-specific cDNA probes showed DDAH1 expression in the amygdala, striatum, cerebellum, thalamus, hippocampus and various cortical areas (Tran et al. 2000). Besides acknowledging the wide mRNA expression in all brain regions tested, protein expression was found to be limited to the cerebral cortex, hippocampus, basal ganglia and cerebellum (Uhlén et al. 2015; Human Protein Atlas 2020). Prior studies in combination with our data, therefore, indicate similar expression profiles between human and murine datasets on both the mRNA and protein level.
Our study revealed a sparser distribution of DDAH2, compared to the widely represented DDAH1, in agreement with western blot analyses showing significantly lower expression of DDAH2 than DDAH1 in murine whole brain samples (Hu et al. 2011). Regional DDAH2 protein expression profile was limited to the cortex, hippocampus, striatum, and pallidum. This profile partially correlated with available mRNA expression data, which also included expression in the hypothalamus and cerebellum (Mouse ENCODE Consortium 2019; Stamatoyannopoulos et al. 2012; Linnarsson Lab 2020; Allen Institute for Brain Science 2010). Similarly, very low DDAH2 mRNA distribution was detected in the human brain (Leiper et al. 1999) or found to be mostly restricted to the medulla and spinal cord (Tran et al. 2000). These findings were again confirmed by mRNA datasets, where DDAH2 mRNA distribution in human brain tissue was barely present compared to the high level of expression observed in the other tissues, e.g., lung and fetal tissue (EMBL-EBI 2020; Human Protein Atlas 2020; Uhlén et al. 2015).
Additionally, we demonstrated for the first time that DDAH2 protein in the adult murine brain is found exclusively in neuronal cell types, which is in line with reported neuronal Ddah2 mRNA expression (Allen Institute for Brain Science 2010; Linnarsson Lab 2020). The neuronal identity of DDAH2 positive cells was also confirmed in our immunocytochemical analysis on primary cell cultures of cortical and hippocampal origin. However, in primary cell culture, DDAH2 was additionally observed in astrocytes. The early postnatal origin of these samples and previous reports of high embryonic DDAH2 expression, which was found to decrease over development (Tran et al. 2000), may explain this finding. Of note, DDAH1 expression remains much more stable during development (Tran et al. 2000).
Studies demonstrating expression of DDAH2 in endothelial cells (Lin et al. 2019; Wang et al. 2007; Pullamsetti et al. 2005) and the hematopoietic lineage (Tran et al. 2000; Winkler et al. 2017; Lambden et al. 2015; Tomikawa et al. 2006) are in contrast with our observation of lacking signal in cerebral endothelial cells and microglia. It has been noted that gene expression patterns of the endothelium are highly heterogeneous and linked to specialized functional roles across vascular beds (Chi et al. 2003; Nolan et al. 2013). Such tissue-specific adaptations for brain endothelial cells include the instruction of neuronal differentiation and blood–brain-barrier formation (Jambusaria et al. 2020). Also, we do not exclude that either one of these cell types, endothelial cells and microglia, does express DDAH2 during non-adult stages. Besides the above-mentioned overall reduction in DDAH2 with age (Tran et al. 2000), a significant developmental decline between P14-P60 of Ddah2 mRNA in microglia was observed (Zhang et al. 2014). Further, single-cell transcriptomics from mouse cortex and neurogenic niches (Zywitza et al. 2018; Zeisel et al. 2015) support low transcript levels in adults, possibly below our detection limits. Finally, upregulation of DDAH2 may occur in response to a stressor and during pathological conditions (Jambusaria et al. 2020; Gunawardana et al. 2021). This possibility is exemplified by the observed brain-specific up-regulation in endothelial transcripts in response to key mediators of tissue inflammation (Cleuren et al. 2019) and the DDAH2 increase in murine monocytes and human peripheral blood mononuclear cells in response to hypoxia (Lambden et al. 2016).
Together, our data indicate a distinct expression of DDAH isoforms in the brain. Such isolated patterns of expression of DDAH isoforms have also been observed in peripheral tissues. In the kidney, for example, DDAH1 is observed in the proximal tubules, while DDAH2 is expressed in the glomeruli, afferent arterioles, macula densa, and distal nephron, where each DDAH isoform has been found to regulate local ADMA concentrations in specific pathophysiologic processes (Onozato et al. 2008; Tomlinson et al. 2015). Observed regional and cellular specific expression profiles of DDAH1 and DDAH2 thus support our hypothesis of different functions or divergent influences on brain activity. Early studies already suggested that DDAH1 is primarily co-expressed with nNOS in the brain and DDAH2 is detected in tissues that also express eNOS and iNOS such as endothelial tissue and immune cells, respectively (Tran et al. 2000; Leiper et al. 1999; Suzuki-Yamamoto et al. 2012). Later it was shown that the DDAH1 expression patterns were completely different from nNOS expression patterns in rat and chicken during embryonic development (Mishima et al. 2004). Our study demonstrates that at least in the adult brain, it is DDAH2, rather than DDAH1, which is neuron specific. By itself, this does not imply nNOS co-expression and further analyses need to be performed for clarification. Additionally, it is possible that the different expression patterns of DDAH isoforms are caused by different regulatory mechanisms and maybe even different substrate preferences of these enzymes (Todd et al. 2001; Nguyen Ba et al. 2014). Indeed, while the major role of DDAH1 in the regulation of ADMA homeostasis is generally accepted, the relative contribution of DDAH2 towards ADMA clearance is still controversial (Wojciak-Stothard et al. 2007; Hasegawa et al. 2007; Hu et al. 2011; Wang et al. 2007). Even the data on whether DDAH2 is enzymatically active towards ADMA at all, are contrasting. On the one hand, global Ddah2 knockout mice exhibit a significant increase in tissue ADMA concentrations (Lambden et al. 2015) and localized Ddah2 upregulation in mice lung results in decreased tissue ADMA concentrations indicating that DDAH2 actively catabolizes ADMA (Aggarwal et al. 2014). Conversely, tissues of global DDAH1 deficient mice had no detectable enzymatic activity towards ADMA despite unchanged DDAH2 protein levels (Hu et al. 2011). In addition, ADMA-hydrolyzing activity was only observed in porcine tissues with high DDAH1 expression but absent in tissues with high DDAH2 expression lacking DDAH1, which would be consistent with DDAH2 being unable to hydrolyze ADMA (Altmann et al. 2012). As the role of DDAH2 in ADMA degradation remains debatable, further investigations of DDAH2 activity on ADMA are warranted to better understand the physiological and pathophysiological roles of this protein.
Alterations in DDAH activity in the human brain may contribute to the development of certain neuropsychiatric disorders. Changes in ADMA levels have been observed in patients with depression (Baranyi et al. 2015; Selley 2004; Braun et al. 2021), bipolar affective disorder (Sağlam Aykut et al. 2012; Braun et al. 2021), migraine (Greco et al. 2015), schizophrenia (Celik et al. 2011; Braun et al. 2021), Parkinson’s disease (Kirbas et al. 2016) and Alzheimer’s disease (Selley 2003). At the same time, there have been only limited advances in the diagnosis and treatment of these disorders in recent decades. Current therapies, mostly in the form of psychopharmacological agents, often lead to insufficient symptom relief and side effects. Thus, there is a need to develop new and targeted treatment strategies that specifically address the pathological processes from which symptoms arise. The described regional and cellular expression pattern of DDAH isoforms may facilitate the identification of specific pathophysiological roles played by these enzymes in the brain.
Further detailed investigations of DDAH isoform mutant mouse models may not only help to understand the functions of the DDAH/ADMA/NO pathway in the brain but also lead to the strategy of isoform-specific modulation of DDAH activity as a potential new therapeutic target for psychiatric disorders.
Data Availability
The data used and analyzed are available from the corresponding author on reasonable request.
References
Aggarwal S, Gross CM, Kumar S, Dimitropoulou C, Sharma S, Gorshkov BA, Sridhar S, Lu Q, Bogatcheva NV, Jezierska-Drutel AJ et al (2014) Dimethylarginine dimethylaminohydrolase II overexpression attenuates LPS-mediated lung leak in acute lung injury. Am J Respir Cell Mol Biol 50:614–625. https://doi.org/10.1165/rcmb.2013-0193OC
Allen Institute for Brain Science (2010) Allen Mouse Brain Atlas. http://mouse.brain-map.org/ March 12, 2020.
Altmann KS, Havemeyer A, Beitz E, Clement B (2012) Dimethylarginine-dimethylaminohydrolase-2 (DDAH-2) does not metabolize methylarginines. ChemBioChem 13:2599–2604. https://doi.org/10.1002/cbic.201200499
Antoniades C, Shirodaria C, Leeson P, Antonopoulos A, Warrick N, Van-Assche T, Cunnington C, Tousoulis D, Pillai R, Ratnatunga C et al (2009) Association of plasma asymmetrical dimethylarginine (ADMA) with elevated vascular superoxide production and endothelial nitric oxide synthase uncoupling: implications for endothelial function in human atherosclerosis. Eur Heart J 30:1142–1150. https://doi.org/10.1093/eurheartj/ehp061
Asif M, Soiza RL, McEvoy M, Mangoni AA (2013) Asymmetric dimethylarginine: a possible link between vascular disease and dementia. Curr Alzheimer Res 10:347–356. https://doi.org/10.2174/1567205011310040001
Ayling LJ, Whitley GSJ, Aplin JD, Cartwright JE (2006) Dimethylarginine dimethylaminohydrolase (DDAH) regulates trophoblast invasion and motility through effects on nitric oxide. Hum Reprodction 21:2530–2537. https://doi.org/10.1093/humrep/del111
Baranyi A, Amouzadeh-Ghadikolai O, Rothenhäusler H-B, Theokas S, Robier C, Baranyi M, Koppitz M, Reicht G, Hlade P, Meinitzer A (2015) Nitric oxide-related biological pathways in patients with major depression. PLoS ONE 10:e0143397–e0143397. https://doi.org/10.1371/journal.pone.0143397
Boger RH (2005) Asymmetric dimethylarginine (ADMA) and cardiovascular disease: insights from prospective clinical trials. Vasc Med 10(Suppl 1):S19-25
Böhme GA, Bon C, Lemaire M, Reibaud M, Piot O, Stutzmann J-M, Doble A, Blanchard J-C (1993) Altered synaptic plasticity and memory formation in nitric oxide synthase inhibitor-treated rats. Proc Natl Acad Sci 90:9191–9194
Braun D, Schlossmann J, Haen E (2021) Asymmetric dimethylarginine in psychiatric disorders. Psychiatry Res 300:113901. https://doi.org/10.1016/j.psychres.2021.113901
Calabrese V, Mancuso C, Calvani M, Rizzarelli E, Butterfield DA, Stella AM (2007) Nitric oxide in the central nervous system: neuroprotection versus neurotoxicity. Nat Rev Neurosci 8:766–775. https://doi.org/10.1038/nrn2214
Celik C, Cayci T, Ozdemir B, Akgul EO, Zincir S, Balikci A, Uzun O, Yaman H (2011) Plasma asymmetric dimethylarginine (ADMA) concentrations in patients with first and multiple episode schizophrenia. Psychiatry Res 190:177–180. https://doi.org/10.1016/j.psychres.2011.06.003
Chen J, Zacharek A, Zhang C, Jiang H, Li Y, Roberts C, Lu M, Kapke A, Chopp M (2005) Endothelial nitric oxide synthase regulates brain-derived neurotrophic factor expression and neurogenesis after stroke in mice. J Neurosci 25:2366–2375. https://doi.org/10.1523/JNEUROSCI.5071-04.2005
Chen S, Li N, Deb-Chatterji M, Dong Q, Kielstein JT, Weissenborn K, Worthmann H (2012) Asymmetric dimethyarginine as marker and mediator in ischemic stroke. Int J Mol Sci 13:15983–16004
Chi J-T, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D et al (2003) Endothelial cell diversity revealed by global expression profiling. Proc Natl Acad Sci 100:10623. https://doi.org/10.1073/pnas.1434429100
Cleuren ACA, van der Ent MA, Jiang H, Hunker KL, Yee A, Siemieniak DR, Molema G, Aird WC, Ganesh SK, Ginsburg D (2019) The in vivo endothelial cell translatome is highly heterogeneous across vascular beds. Proc Natl Acad Sci 116:23618. https://doi.org/10.1073/pnas.1912409116
Dayal S, Rodionov RN, Arning E, Bottiglieri T, Kimoto M, Murry DJ, Cooke JP, Faraci FM, Lentz SR (2008) Tissue-specific downregulation of dimethylarginine dimethylaminohydrolase in hyperhomocysteinemia. Am J Physiol 295:H816–H825. https://doi.org/10.1152/ajpheart.01348.2007
de Sousa AR, Penalva LO, Marcotte EM, Vogel C (2009) Global signatures of protein and mRNA expression levels. Mol Biosyst 5:1512–1526. https://doi.org/10.1039/b908315d
Dhir A, Kulkarni SK (2011) Nitric oxide and major depression. Nitric Oxide 24:125–131. https://doi.org/10.1016/j.niox.2011.02.002
D’Mello R, Sand CA, Pezet S, Leiper JM, Gaurilcikaite E, McMahon SB, Dickenson AH, Nandi M (2015) Dimethylarginine dimethylaminohydrolase 1 is involved in spinal nociceptive plasticity. Pain 156:2052–2060. https://doi.org/10.1097/j.pain.0000000000000269
Dowsett L, Piper S, Slaviero A, Dufton N, Wang Z, Boruc O, Delahaye M, Colman L, Kalk E, Tomlinson J et al (2015) Endothelial dimethylarginine dimethylaminohydrolase 1 is an important regulator of angiogenesis but does not regulate vascular reactivity or hemodynamic homeostasis. Circulation 131:2217–2225. https://doi.org/10.1161/CIRCULATIONAHA.114.015064
EMBL-EBI (2020) HUGO Gene Nomenclature Commitee. https://www.genenames.org/ September 9, 2020.
Freudenberg F, Alttoa A, Reif A (2015) Neuronal nitric oxide synthase (NOS1) and its adaptor, NOS1AP, as a genetic risk factors for psychiatric disorders. Genes Brain Behav 14:46–63. https://doi.org/10.1111/gbb.12193
Frey D, Braun O, Briand C, Vašák M, Grütter MG (2006) Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: a basis for the design of specific inhibitors. Structure 14:901–911. https://doi.org/10.1016/j.str.2006.03.006
Gang Zhang Z, Chopp M, Gautman S, Zaloga C, Lan Zhang R, Schmidt HHHW, Pollack JS, Förstermann U (1994) Upregulation of neuronal nitric oxide synthase and mRNA, and selective sparing of nitric oxide synthase-containing neurons after local cerebral ischemia in rat. Brain Res 654:85–95. https://doi.org/10.1016/0006-8993(94)91574-1
Gatto EM, Riobó NA, Carreras MC, Cherñavsky A, Rubio A, Satz ML, Poderoso JJ (2000) Overexpression of neutrophil neuronal nitric oxide synthase in parkinson’s disease. Nitric Oxide 4:534–539. https://doi.org/10.1006/niox.2000.0288
Greco R, Ferrigno A, Demartini C, Zanaboni A, Mangione AS, Blandini F, Nappi G, Vairetti M, Tassorelli C (2015) Evaluation of ADMA-DDAH-NOS axis in specific brain areas following nitroglycerin administration: study in an animal model of migraine. J Headache Pain 16:560. https://doi.org/10.1186/s10194-015-0560-2
Gunawardana H, Romero T, Yao N, Heidt S, Mulder A, Elashoff DA, Valenzuela NM (2021) Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses. Sci Rep 11:1949. https://doi.org/10.1038/s41598-020-80102-w
Hannemann J, Appel D, Seeberger-Steinmeister M, Brüning T, Zummack J, Böger R (2020) Sequence variation in the DDAH1 gene predisposes for delayed cerebral ischemia in subarachnoidal hemorrhage. J Clin Med 9:3900
Hasegawa K, Wakino S, Tatematsu S, Yoshioka K, Homma K, Sugano N, Kimoto M, Hayashi K, Itoh H (2007) Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2. Circ Res 101:e2–e10. https://doi.org/10.1161/circresaha.107.156901
Hölscher C, McGlinchey L, Anwyl R, Rowan MJ (1996) 7-Nitro indazole, a selective neuronal nitric oxide synthase inhibitor in vivo, impairs spatial learning in the rat. Learn Mem 2:267–278
Hu X, Atzler D, Xu X, Zhang P, Guo H, Lu Z, Fassett J, Schwedhelm E, Boger RH, Bache RJ et al (2011) Dimethylarginine dimethylaminohydrolase-1 is the critical enzyme for degrading the cardiovascular risk factor asymmetrical dimethylarginine. Arterioscler Thromb Vasc Biol 31:1540–1546. https://doi.org/10.1161/atvbaha.110.222638
Human Protein Atlas (2020) Human Protein Atlas. http://www.proteinatlas.org September 9, 2020.
Izumi Y, Clifford D, Zorumski C (1992) Inhibition of long-term potentiation by NMDA-mediated nitric oxide release. Science 257:1273–1276. https://doi.org/10.1126/science.1519065
Jambusaria A, Hong Z, Zhang L, Srivastava S, Jana A, Toth PT, Dai Y, Malik AB, Rehman J (2020) Endothelial heterogeneity across distinct vascular beds during homeostasis and inflammation. Elife. https://doi.org/10.7554/eLife.51413
Kakimoto Y, Akazawa S (1970) Isolation and identification of Ng, Ng- and Ng, N’g-dimethylarginine, Nε-mono-, Di-, and trimethyllysine, and glucosylgalactosyl- and galactosyl-δ-hydroxylysine from human urine. J Biol Chem 245:5751–5758
Kirbas S, Kirbas A, Tufekci A, Cumhur Cure M, Cakmak S, Yazici T, Cure E (2016) Serum levels of homocysteine, asymmetric dimethylarginine and nitric oxide in patients with Parkinson’s disease. Acta Clin Belg 71:71–75. https://doi.org/10.1080/17843286.2016.1138592
Lambden S, Kelly P, Ahmetaj-Shala B, Wang Z, Lee B, Nandi M, Torondel B, Delahaye M, Dowsett L, Piper S et al (2015) Dimethylarginine dimethylaminohydrolase 2 regulates nitric oxide synthesis and hemodynamics and determines outcome in polymicrobial sepsis. Arteriosc Thromb Vasc Biol 35:1382–1392. https://doi.org/10.1161/ATVBAHA.115.305278
Lambden S, Martin D, Vanezis K, Lee B, Tomlinson J, Piper S, Boruc O, Mythen M, Leiper J (2016) Hypoxia causes increased monocyte nitric oxide synthesis which is mediated by changes in dimethylarginine dimethylaminohydrolase 2 expression in animal and human models of normobaric hypoxia. Nitric Oxide 58:59–66. https://doi.org/10.1016/j.niox.2016.06.003
Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P (1999) Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 343(Pt 1):209–214
Leone A, Moncada S, Vallance P, Calver A, Collier J (1992) Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 339:572–575. https://doi.org/10.1016/0140-6736(92)90865-Z
Lin HH, Lee TS, Lin SJ, Yeh YC, Lu TM, Hsu CP (2019) DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase. Clin Sci 133:2361–2378. https://doi.org/10.1042/cs20190455
Linnarsson Lab (2020) Mouse Brain Atlas. http://mousebrain.org/ March 22, 2020.
MacAllister RJ, Parry H, Kimoto M, Ogawa T, Russell RJ, Hodson H, Whitley GS, Vallance P (1996) Regulation of nitric oxide synthesis by dimethylarginine dimethylaminohydrolase. Br J Pharmacol 119:1533–1540. https://doi.org/10.1111/j.1476-5381.1996.tb16069.x
Martens-Lobenhoffer J, Bode-Böger SM, Clement B (2016) First detection and quantification of Nδ-monomethylarginine, a structural isomer of NG-monomethylarginine, in humans using MS3. Anal Biochem 493:14–20. https://doi.org/10.1016/j.ab.2015.10.001
McDermott JR (1976) Studies on the catabolism of Ng-methylarginine, Ng, Ng-dimethylarginine and Ng, Ng-dimethylarginine in the rabbit. Biochem J 154:179–184. https://doi.org/10.1042/bj1540179
Meyer J, Richter N, Hecker M (1997) High-performance liquid chromatographic determination of nitric oxide synthase-related arginine derivativesin vitroandin vivo. Anal Biochem 247:11–16. https://doi.org/10.1006/abio.1997.2008
Mishima T, Hamada T, Ui-Tei K, Takahashi F, Miyata Y, Imaki J, Suzuki H, Yamashita K (2004) Expression of DDAH1 in chick and rat embryos. Brain Res Dev Brain Res 148:223–232. https://doi.org/10.1016/j.devbrainres.2003.09.021
Miyazaki H, Matsuoka H, Cooke JP, Usui M, Ueda S, Okuda S, Imaizumi T (1999) Endogenous nitric oxide synthase inhibitor: a novel marker of atherosclerosis. Circulation 99:1141–1146. https://doi.org/10.1161/01.cir.99.9.1141
Mouse ENCODE Consortium (2019) Mouse ENCODE Project. http://www.mouseencode.org March 22, 2020.
Nguyen Ba AN, Strome B, Hua JJ, Desmond J, Gagnon-Arsenault I, Weiss EL, Landry CR, Moses AM (2014) Detecting functional divergence after gene duplication through evolutionary changes in posttranslational regulatory sequences. PLOS Comput Biol. https://doi.org/10.1371/journal.pcbi.1003977
Niewmierzycka A, Clarke S (1999) S-Adenosylmethionine-dependent methylation in Saccharomyces cerevisiae: identification of a novel protein arginine methyltransferase. J Biol Chem 274:814–824. https://doi.org/10.1074/jbc.274.2.814
Nolan DJ, Ginsberg M, Israely E, Palikuqi B, Poulos MG, James D, Ding B-S, Schachterle W, Liu Y, Rosenwaks Z et al (2013) Molecular signatures of tissue-specific microvascular endothelial cell heterogeneity in organ maintenance and regeneration. Dev Cell 26:204–219. https://doi.org/10.1016/j.devcel.2013.06.017
Ogawa T, Kimoto M, Sasaoka K (1987) Occurrence of a new enzyme catalyzing the direct conversion of NG, NG-dimethyl-L-arginine to L-citrulline in rats. Biochem Biophys Res Commun 148:671–677. https://doi.org/10.1016/0006-291x(87)90929-6
Ogawa T, Kimoto M, Sasaoka K (1989) Purification and properties of a new enzyme, NG, NG-dimethylarginine dimethylaminohydrolase, from rat kidney. J Biol Chem 264:10205–10209
Onozato ML, Tojo A, Leiper J, Fujita T, Palm F, Wilcox CS (2008) Expression of NG, NG-dimethylarginine dimethylaminohydrolase and protein arginine N-methyltransferase isoforms in diabetic rat kidney: effects of angiotensin II receptor blockers. Diabetes 57:172–180. https://doi.org/10.2337/db06-1772
Ozden A, Angelos H, Feyza A, Elizabeth W, John P (2020) Altered plasma levels of arginine metabolites in depression. J Psychiatr Res 120:21–28. https://doi.org/10.1016/j.jpsychires.2019.10.004
Pullamsetti S, Kiss L, Ghofrani HA, Voswinckel R, Haredza P, Klepetko W, Aigner C, Fink L, Muyal JP, Weissmann N et al (2005) Increased levels and reduced catabolism of asymmetric and symmetric dimethylarginines in pulmonary hypertension. FASEB J 19:1175–1177. https://doi.org/10.1096/fj.04-3223fje
Sağlam Aykut D, Tiryaki A, Karagüzel E, Karahan C (2012) Nitric oxide and asymmetrical dimethylarginine levels in acute mania. Bull Clin Psychopharmacol. https://doi.org/10.5455/bcp.20111212083708
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675. https://doi.org/10.1038/nmeth.2089
Selley ML (2003) Increased concentrations of homocysteine and asymmetric dimethylarginine and decreased concentrations of nitric oxide in the plasma of patients with Alzheimer’s disease. Neurobiol Aging 24:903–907. https://doi.org/10.1016/s0197-4580(03)00007-1
Selley ML (2004) Increased (E)-4-hydroxy-2-nonenal and asymmetric dimethylarginine concentrations and decreased nitric oxide concentrations in the plasma of patients with major depression. J Affect Disord 80:249–256. https://doi.org/10.1016/S0165-0327(03)00135-6
Son H, Hawkins RD, Martin K, Kiebler M, Huang PL, Fishman MC, Kandel ER (1996) Long-term potentiation is reduced in mice that are doubly mutant in endothelial and neuronal nitric oxide synthase. Cell 87:1015–1023
Sonar SA, Lal G (2019) The iNOS activity during an immune response controls the CNS pathology in experimental autoimmune encephalomyelitis. Front Immunol 10:710–710. https://doi.org/10.3389/fimmu.2019.00710
Stamatoyannopoulos JA, Snyder M, Hardison R, Ren B, Gingeras T, Gilbert DM, Groudine M, Bender M, Kaul R, Canfield T et al (2012) An encyclopedia of mouse DNA elements (Mouse ENCODE). Genome Biol 13:418. https://doi.org/10.1186/gb-2012-13-8-418
Suzuki-Yamamoto T, Ito A, Yokoro M, Suzuki M, Kimoto M (2012) Immunohistochemical localization of NNOS, PRMT and DDAH in the rat central nervous system. Nitric Oxide 27:S20. https://doi.org/10.1016/j.niox.2012.04.070
The Human Brain (2020) The Human Brain Coronal Atlas. http://www.thehumanbrain.info/head_brain/coronal.php March 12, 2020.
Todd AE, Orengo CA, Thornton JM (2001) Evolution of function in protein superfamilies, from a structural perspective. J Mol Biol 307:1113–1143. https://doi.org/10.1006/jmbi.2001.4513
Tomikawa J, Fukatsu K, Tanaka S, Shiota K (2006) DNA methylation-dependent epigenetic regulation of dimethylarginine dimethylaminohydrolase 2 gene in trophoblast cell lineage. J Biol Chem 281:12163–12169. https://doi.org/10.1074/jbc.M513782200
Tomlinson JA, Caplin B, Boruc O, Bruce-Cobbold C, Cutillas P, Dormann D, Faull P, Grossman RC, Khadayate S, Mas VR et al (2015) Reduced renal methylarginine metabolism protects against progressive kidney damage. J Am Soc Nephrol 26:3045–3059. https://doi.org/10.1681/asn.2014030280
Toth J, Racz A, Kaminski PM, Wolin MS, Bagi Z, Koller A (2007) Asymmetrical dimethylarginine inhibits shear stress-induced nitric oxide release and dilation and elicits superoxide-mediated increase in arteriolar tone. Hypertension 49:563–568. https://doi.org/10.1161/01.HYP.0000256764.86208.3d
Tran CTL, Fox MF, Vallance P, Leiper JM (2000) Chromosomal localization, gene structure, and expression pattern of DDAH1: comparison with DDAH2 and implications for evolutionary origins. Genomics 68:101–105. https://doi.org/10.1006/geno.2000.6262
Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A et al (2015) Tissue-based map of the human proteome. Science 347:1260419. https://doi.org/10.1126/science.1260419
Ustundag MF, Ozcan H, Gencer AG, Yilmaz ED, Uğur K, Oral E, Bilici M (2020) Nitric oxide, asymmetric dimethylarginine, symmetric dimethylarginine and L-arginine levels in psychotic exacerbation of schizophrenia and bipolar disorder manic episode. Saudi Med J 41:38–45. https://doi.org/10.15537/smj.2020.1.24817
Usui M, Matsuoka H, Miyazaki H, Ueda S, Okuda S, Imaizumi T (1998) Increased endogenous nitric oxide synthase inhibitor in patients with congestive heart failure. Life Sci 62:2425–2430. https://doi.org/10.1016/s0024-3205(98)00225-2
Vallance P, Leone A, Calver A, Collier J, Moncada S (1992) Endogenous dimethylarginine as an inhibitor of nitric oxide synthesis. J Cardiovasc Pharmacol 20(Suppl 12):S60–S62
Wang D, Gill PS, Chabrashvili T, Onozato ML, Raggio J, Mendonca M, Dennehy K, Li M, Modlinger P, Leiper J et al (2007) Isoform-specific regulation by NG, NG-dimethylarginine dimethylaminohydrolase of rat serum asymmetric dimethylarginine and vascular endothelium-derived relaxing factor/NO. Circ Res 101:627–635. https://doi.org/10.1161/circresaha.107.158915
Winkler MS, Kluge S, Holzmann M, Moritz E, Robbe L, Bauer A, Zahrte C, Priefler M, Schwedhelm E, Böger RH et al (2017) Markers of nitric oxide are associated with sepsis severity: an observational study. Crit Care 21:189. https://doi.org/10.1186/s13054-017-1782-2
Wojciak-Stothard B, Torondel B, Tsang LY, Fleming I, Fisslthaler B, Leiper JM, Vallance P (2007) The ADMA/DDAH pathway is a critical regulator of endothelial cell motility. J Cell Sci 120:929–942. https://doi.org/10.1242/jcs.002212
Zeisel A, Muñoz-Manchado AB, Codeluppi S, Lönnerberg P, La Manno G, Juréus A, Marques S, Munguba H, He L, Betsholtz C et al (2015) Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq. Science 347:1138. https://doi.org/10.1126/science.aaa1934
Zhang Y, Chen K, Sloan SA, Bennett ML, Scholze AR, Keeffe S, Phatnani HP, Guarnieri P, Caneda C, Ruderisch N et al (2014) An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neurosci 34:11929. https://doi.org/10.1523/JNEUROSCI.1860-14.2014
Zhou QG, Hu Y, Hua Y, Hu M, Luo CX, Han X, Zhu XJ, Wang B, Xu JS, Zhu DY (2007) Neuronal nitric oxide synthase contributes to chronic stress-induced depression by suppressing hippocampal neurogenesis. J Neurochem 103:1843–1854. https://doi.org/10.1111/j.1471-4159.2007.04914.x
Zobel-Thropp P, Gary JD, Clarke S (1998) Delta N-methylarginine is a novel posttranslational modification of arginine residues in yeast proteins. J Biol Chem 273:29283–29286. https://doi.org/10.1074/jbc.273.45.29283
Zoccali C, Bode-Böger SM, Mallamaci F, Benedetto FA, Tripepi G, Malatino LS, Cataliotti A, Bellanuova I, Fermo I, Frölich JC et al (2001) Plasma concentration of asymmetrical dimethylarginine and mortality in patients with end-stage renal disease: a prospective study. Lancet 358:2113–2117. https://doi.org/10.1016/S0140-6736(01)07217-8
Zywitza V, Misios A, Bunatyan L, Willnow TE, Rajewsky N (2018) Single-cell transcriptomics characterizes cell types in the subventricular zone and uncovers molecular defects impairing adult neurogenesis. Cell Rep 25:2457–2469.e8. https://doi.org/10.1016/j.celrep.2018.11.003
Acknowledgements
We thank Ekaterina S. Karetnikova, Kristin Wogan and the Core Facility Cellular Imaging (TU Dresden) for their excellent technical support. We are grateful to Anna S. Artemyeva and the St. Petersburg State University Research Park “Chromas” (Human tissue collection) for human tissue sampling and the technical support of the experiments. Further, we acknowledge Prof. Georg Breier and Anke Klawitter for providing antibody, Dr. Eva Szego and Prof. Björn Falkenburger for providing primary cell culture samples and Prof. Yingjie Chen for providing global DDAH1 deficient mice.
Funding
Open Access funding enabled and organized by Projekt DEAL. This work was supported through the woman habilitation promotion initiative from the Medical Faculty awarded to NB. AAK received research scholarships from the German Academic Exchange Service and the Gesellschaft von Freunden und Förderern der TU Dresden. Further, AAK and RRG received support through the project ID: 51143531 of the St. Petersburg State University, St. Petersburg, Russia. RNR was supported by funding from the German Heart Foundation/German Foundation of Heart Research F/24/17.
Author information
Authors and Affiliations
Contributions
AAK performed experiments on mouse and human tissue, conducted data analysis and interpretation. VNR and ER performed experiments on peripheral cell lines and antibody validation. IVL performed experiments on mouse brain primary cell cultures. AEB, AGM and AAM contributed to human tissue experiments, analysis, and data interpretation. NB conceived the study and designed experiments. RNR designed experiments and with RRG, MB, NW, TS-Y, MK provided supervision and data interpretation. AAK, VNR, NJ and NB wrote the manuscript. All authors carefully revised the first draft and read and approved the final version.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Ethical approval
Animal experiments were carried out in accordance to the European Communities Council Directive of 22nd September 2010 (2010/63/EU) under protocols approved by the animal ethics committees of the Technische Universität Dresden and the Landesdirektion Sachsen (ID TVV5/2019).
Informed Consent
Human tissue analysis was carried out in accordance with the St. Petersburg University Ethics Committee and the Southern Adelaide Health Service / Flinders University Human Research Ethics Committee (Project ID: 181/067).
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Alena A. Kozlova and Vinitha N. Ragavan contributed equally as co-first authors.
Roman N. Rodionov and Nadine Bernhardt contributed equally to this work.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Kozlova, A.A., Ragavan, V.N., Jarzebska, N. et al. Divergent Dimethylarginine Dimethylaminohydrolase Isoenzyme Expression in the Central Nervous System. Cell Mol Neurobiol 42, 2273–2288 (2022). https://doi.org/10.1007/s10571-021-01101-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10571-021-01101-7