Abstract
The platelet-derived growth factor-C (PDGF-C) gene of Gekko japonicus was obtained from a brain and spinal cord cDNA library. The results of Northern blot showed that transcript of PDGF-C gene of gecko is 2.8 kb in length, and it was abundantly expressed in tissues of heart, lung, kidney, and ovary. In situ hybridization (ISH) revealed that positive hybridization signals were present in both gray matter and white matter of the spinal cord. The change of PDGF-C expression in the spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The expression of PDGF-C in the spinal cord showed highest level at 1 day after tail amputation, and gradually decreased until 2 weeks, which indicated that the expression level of PDGF-C might be associated with the process of spinal cord injury and regeneration.
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Acknowledgments
This research was supported by a grant from the National Basic Research Program (973 Program) of Ministry of Science and Technology of China (2003CB515306), a grant from Basic Research Program of Jiangsu Education Department (05KJA31010) and a grant from the National Natural Science Foundation of China (30600172).
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Yan Liu and Zheng Fan have contributed equally to this work.
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Liu, Y., Fan, Z., Zhou, Y. et al. The Molecular Cloning of Platelet-Derived Growth Factor-C (PDGF-C) Gene of Gekko japonicus and Its Expression Change in the Spinal Cord After Tail Amputation. Cell Mol Neurobiol 29, 263–271 (2009). https://doi.org/10.1007/s10571-008-9319-5
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DOI: https://doi.org/10.1007/s10571-008-9319-5