Abstract
Ethyl lactate was produced with corn stover as a carbon source by a cascade bioprocess without adding any enzymes. Immobilized Aspergillus niger and Saccharomyces cerevisiae was co-cultivated in a modified gas lift bioreactor. Cellulase from Aspergillus niger was 8.05 U/mL in the gas lift bioreactor. The fermentation broth was transferred into an anaerobic fermenter, in which the immobilized Saccharomyces cerevisiae and Lactobacillus rhamnosus was cultivated. The maximum esterase activities were over 5005 U/mL and the ethyl lactate yield was over 8.76 g/L during five repeated batches. Without adding any cellulase and esterase, ethyl lactate production was achieved during five repeated batches by the cascade of the aerobic process and the anaerobic process with the lignocellulose as a carbon source.
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Acknowledgments
The authors would like to thank the National Natural Science Foundation of China and the HBUT National ‘‘111’’ Center for Cellular Regulation and Molecular Pharmaceutics for the financial support.
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This work was supported by the National Natural Science Foundation of China (32070107), and the Collaborative Grant-in-Aid of the HBUT National ‘‘111’’ Center for Cellular Regulation and Molecular Pharmaceutics.
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XD, XX, XT and HJ wrote and revised the main manuscript text. JB and HF prepared figures. YX reviewed the manuscript. GC: supervised and review the study. All authors reviewed the manuscript.
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Xue, D., Xing, X., Jiang, B. et al. Ethyl lactate biosynthesis by the cascade of the aerobic process and the anaerobic process with corn stover. Cellulose 31, 1497–1508 (2024). https://doi.org/10.1007/s10570-023-05729-0
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DOI: https://doi.org/10.1007/s10570-023-05729-0