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Enhanced production of bacterial cellulose by Komactobacter intermedius using statistical modeling

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Abstract

The extraordinary nature of the bacterial cellulose (BC) biopolymer gives it potential for diverse applications; however, the low BC yield of many indigenous cellulose-producing bacteria is a persistent problem in its synthesis. In this study, the BC yield of Komactobacter intermedius (BCRC 910677) was optimized by modifying culture media. The optimal culture period, type of carbon, and nitrogen sources were evaluated using the one-factor-at-a-time approach prior to the optimization study. The optimization was done by using the response surface methodology (RSM). In RSM optimization study, a Box–Behnken design with three parameters is applied; the three parameters include fructose concentrations (X1), peptone concentrations (X2), and pH values (X3). Our optimal culture media combined 41 g/L of fructose, 38 g/L peptone, and a pH of 5.2. The predicted BC yield from the RSM model is 4.012 g/L, while BC yield of 3.906 g/L is obtained from the experiment using the optimized medium; that is only 2.64% difference. An increase in BC production of 3.82-fold (compared to the culture in HS medium) was obtained after 6-days culture. The K. intermedius investigated in this study show great potential for commercial BC productions and as feedstock. The RSM can be a promising approach to enhance BC yield since the parameters were well correlated.

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Acknowledgments

This study was partly funded by the Ministry of Science and Technology, Taiwan, under Contract No. 106-2628-E-002-009-MY3.

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Author notes

  1. Shella Permatasari Santoso and Chih-Chan Chou have contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya, East Java, 60114, Indonesia

    Shella Permatasari Santoso, Felycia Edi Soetaredjo & Suryadi Ismadji

  2. Department of Chemical Engineering, National Taiwan University of Science and Technology, #43, Sec. 4, Keelung Rd., Taipei, 10607, Taiwan

    Shella Permatasari Santoso, Felycia Edi Soetaredjo & Suryadi Ismadji

  3. Institute of Biotechnology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan

    Chih-Chan Chou & Kuan Chen Cheng

  4. Department of Food Safety, Taipei Medical University, Taipei, 11042, Taiwan

    Shin-Ping Lin

  5. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan

    Chang-Wei Hsieh

  6. Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan

    Kuan Chen Cheng

  7. Department of Medical Research, China Medical University Hospital, China Medical University, No. 91, Hsueh-Shih Rd., Taichung, 40402, Taiwan

    Kuan Chen Cheng

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  1. Shella Permatasari Santoso
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Correspondence to Kuan Chen Cheng.

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Santoso, S.P., Chou, CC., Lin, SP. et al. Enhanced production of bacterial cellulose by Komactobacter intermedius using statistical modeling. Cellulose 27, 2497–2509 (2020). https://doi.org/10.1007/s10570-019-02961-5

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