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Homogeneous cellulose thin films by regeneration of cellulose xanthate: properties and characterization


The preparation and characterization of cellulose thin films derived from cellulose xanthate is reported. The films are prepared by depositing alkaline aqueous solutions of cellulose xanthate onto silicon wafers, followed by a spin coating step. Depending on the xanthate concentration used for spin coating, films with 50 and 700 nm thickness are obtained. The cellulose xanthate is converted to cellulose by exposing the films to HCl vapors over a period of 20 min. The conversion is monitored by ATR-IR spectroscopy, which allows for tracking the rupture of C–S and C=S bonds during the regeneration process. The conversion is accompanied by a reduction of the film thickness of ca 40% due to the removal of the bulky xanthate group. The films feature a homogenous, but porous morphology as shown by atomic force microscopy. Further, the films were investigated towards their interaction with Bovine Serum Albumin (BSA) and fibrinogen by means of multi-parameter surface plasmon resonance spectroscopy. Similar as other cellulose thin films BSA adsorption is low while fibrinogen adsorbs to some extent at physiological pH (7.4).


In recent years, cellulose thin films have attracted significant interest in both basic and applied research since they provide a confined two dimensional environment with defined morphology and chemistry (Kontturi et al. 2003, 2006, 2013). Therefore, they have been proposed as supports for biosensors, as matrix for catalysis as well as material for optoelectronic devices such as transistors and solar cells (Blomstedt et al. 2007; Filpponen et al. 2012; Niegelhell et al. 2016; Orelma et al. 2011, 2012; Reishofer et al. 2017; Taajamaa et al. 2013; Wolfberger et al. 2015). Further, these films have been employed as models to investigate and to better understand interactions of water with cellulose, particularly in the context of cell wall structure and their relation to industrially applied drying processes (Ehmann et al. 2015; Niinivaara et al. 2015, 2016).

The strategies to prepare such films are based on three main approaches which comprise either (1) the use of cellulose suspensions, (2) the dissolution of cellulose or (3) the employment of cellulose derivatives which are converted back to cellulose after the film forming process (Kontturi et al. 2006). The advantage of using suspensions is to avoid the use of organic solvents but the obtained films are not completely flat due to the (nano)fibrous or particulate nature of the cellulosic starting materials. In contrast, the preparation of cellulose thin films derived from dissolved cellulose requires tedious dissolution procedures (e.g. DMA/LiCl or ionic liquids) (Kargl et al. 2015). Additionally, the solvent (and/or salt) needs to be removed from the films, which necessitates extensive washing procedures of the films. An elegant method to prepare cellulose thin films overcoming these problems has been introduced by Klemm in 1993 and further developed by Kontturi in the beginning of the century. (Kontturi et al. 2003; Schaub et al. 1993) The method employs an acid labile organosoluble cellulose derivative, trimethylsilyl cellulose (TMSC), which is converted to cellulose after processing by simple exposure to HCl vapors. Since the whole process runs via gas phase reaction and since only volatile side products are formed, washing steps are not necessary. In addition, the solubility of this cellulose derivative can be tuned by variation of the DSTMS and therefore allows easy manufacturing, e.g. by spin coating, the most common thin film formation technique. The films obtained by this process are smooth and featureless with RMS roughnesses of down to 1.5 nm as determined by atomic force microscopy. However, although TMSC is a commercial cellulose derivative, whose production is in principle scalable, it is a rather expensive material. Although eco-solvents such as ethyl acetate have been proposed as solvents for TMSC, the use of an aqueous cellulose solution for film formation would be desirable (Wolfberger et al. 2014, 2015).

In this context, a widely used, water soluble cellulose derivative, which can be easily converted back to cellulose, is cellulose xanthate (CX). CX has been first prepared already in the end of the nineteenth century and was since then used as a soluble cellulose derivative for the production of regenerated fibers and films. In a typical fiber/film producing process, the xanthates are regenerated by immersion in an acidic bath which in addition contains some additives to improve fiber/film properties. Nowadays, the market for viscose based products is steadily increasing by ca 10% year whereas specialities become more and more attractive to manufactures (Götze 1951; Hämmerle 2011).

For all these reasons, we became interested whether cellulose xanthate can be employed for the formation of thin cellulose films having film thicknesses below 100 nm. Cellulose thin films from TMSC have shown potential for some applications but the use of CX as starting material may lead to new materials with interesting properties while being biobased and scalable to large quantities. The resulting films could then either be converted by simple immersion in an acidic bath to regenerate the cellulose or to expose them to an acidic atmosphere (e.g. HCl) similar to TMSC films. In the following, we report our approach to prepare such films starting from different concentrations of cellulose xanthate in aqueous alkaline solutions and their influence on film properties.



A cellulose xanthate (CX) stock solution (10 wt%, gamma: 52, NaOH: 6%, degree of polymerization: 550) was provided by Lenzing AG (Lenzing, Austria) and used without any further treatment. CX solutions with 0.75, 1.0, 1.5, 2.0, 2.5, 3.0 and 4.0 wt% were prepared by adding de-ionized water from an Elga PURELAB Prima (Bucks, United Kingdom) water treatment system to the stock solutions. Afterwards, the solutions were vigorously mixed using a vortex shaker over a period of 30 s, followed by a filtration step. Sulfuric acid (95 wt%) and hydrochloric acid (37 wt%) were purchased from VWR Chemicals and hydrogen peroxide (30 wt%) from Sigma–Aldrich. Silicon wafers, glass slides (Roth), Au-coated glass slides as substrate (SPR102-AU), Filter Chromafil Xtra PVDF-45/25 0.45 μm were used as obtained. BSA and fibrinogen were purchased from Sigma, Aldrich.

Cellulose thin film preparation

As substrates for the cellulose films, single side polished silicon wafers from Siegert Wafers (Aachen, Germany, wafer thickness: 675 ± 25 μm, 1 cm × 1 cm) and gold coated glass slides from BioNavis (Tampere, Finland, gold layer thickness: 50 nm, 1 cm × 2 cm) were used. The slides were cleaned by dipping them into piranha acid [H2SO4:H2O2 = 3:1 (v/v)] for 30 min (10 min for gold slides) and intensely washed with MilliQ water afterwards.

For spin coating, 80 μl of viscose solution (per square centimeter substrate) were deposited onto the surfaces and subjected to spin coating (a = 2500 rpm s−1, v = 4000 rpm, t = 60 s). Afterwards, the thin films were stored at room temperature overnight, followed by regeneration of the deposited CX layers in vaporous HCl atmosphere (created from concentrated hydrochloric acid). During the regeneration procedure, the HCl was deposited into a petri dish, and the substrates were positioned above the liquid HCl phase in another petri dish. A third petri dish was used as a lid to close the system. After 20 min exposure to the gaseous HCl atmosphere, regeneration of the cellulose xanthates to cellulose is accomplished as proven by ATR-IR spectroscopy. Afterwards, the films were rinsed twice with water (3 ml) followed by drying in a stream of dry nitrogen.

Optical microscopy

Light microscopy investigations were carried out on an Olympus BX60 fitted with an Olympus E-520 camera. Prior to investigating the samples, carrier substrates were fixed on a glass layer.


The layer thickness was determined with a Bruker DekTak XT surface profiler. The scan length was set to 1000 μm over the time duration of 3 s with the hills and valleys scanning profile. The diamond stylus had a radius of 12.5 μm and the employed force was 3 mg. The measured profile was then used to determine the thickness. Each layer thickness has been determined by averaging 6 measurements on three different slides.

Attenuated total reflection: infrared spectroscopy

The infrared spectra were recorded with an ALPHA FT-IR spectrometer (Bruker; Billerica, MA, U.S.A.). For the measurement, an attenuated total reflection (ATR) attachment was used with 64 scans at a resolution of 4 cm−1 and a scan range between 4000 and 400 cm−1. The samples were prepared on Au-coated glass slides (SPR102-AU). The data were analyzed with OPUS 4.0 software.

Atomic force microscopy

The atomic force microscopy (AFM) images were recorded in tapping mode with a Veeco Multimode Quadrax MM AFM (Bruker; Billerica, MA, USA). For the measurements silicon cantilevers (NCH-VS1-W from NanoWorld AG, Neuchatel, Switzerland; Coating: none) with a resonance frequency of 297 kHz were used. All measurements were performed under ambient atmosphere and at room temperature. The image processing and the calculation of the root mean square roughness (calculated from 2 μm × 2 μm and 10 μm × 10 μm images) were done with the Nanoscope software (V7.30r1sr3; Veeco).

Contact angle and surface free energy determination

For the calculation of the surface free energy (SFE) Milli-Q water (≥ 18 MΩ cm−1) and diiodomethane were employed as test liquids. The drop shape analysis was done in the sessile drop modus at 25 °C with a DSA100 system (Krüss GmbH, Hamburg, Germany) equipped with a T1E CCD video camera (25 fps). The dispense rate of the 3 μl droplets was adjusted to 166 μl/min and the time before the image was captured was set to 2 s. Each sample was measured at least three times. The contact angle (CA) calculations (software: DSA1 v 1.90) were performed with the Young–Laplace equation and the surface free energy calculation with the Owens–Wendt–Rabel and Kaelble method (Owens and Wendt 1969).

Scanning electron microscopy: energy-dispersive X-ray spectroscopy

An ESEM Quanta 600 FEG (FEI, Eindhoven, NL) scanning electron microscope was utilized to examine the surface structure during different steps of film preparation. To gain electrical conductivity a 15 nm thick carbon layer was vaporized on the film surface before. Images were recorded in the high vacuum mode either with secondary electrons (ETD Everhart–Thornley detector, giving topographic contrast) or with backscattered electrons (SSD: Solid State Detector, delivering compositional contrast).

Multi parameter surface plasmon resonance spectroscopy

MP-SPR spectroscopy was accomplished with a SPR Navi 200 from Bionavis Ltd., Tampere, Finland, equipped with two different lasers (670 and 785 nm, respectively) in both measurement channels, using gold-coated glass slides as substrate (gold layer 50 nm). All measurements were performed using a full angular scan (39°–78°, scan speed: 8° s−1). Protein adsorption was investigated with Bovine Serum Albumin (BSA) (1 mg ml−1) and fibrinogen (FIB) (1 mg ml−1) both in a 100 mM NaCl solution containing 10 mM PBS at pH 7.4. Flow was adjusted to 50 μl min−1 for 5 min at 25 °C. Each measurement was repeated 3 times at least. BioNavis Dataviewer software was used for processing all two wavelength SPR experiments (Scheme 1).

Scheme 1
scheme 1

Cellulose thin film production based on aqueous cellulose xanthate (‘Viscose’) solutions

Results and discussion

In a first step, the film formation properties of CX solutions by spin coating were investigated as a function of the CX concentration. It turned out that the CX concentration must be in a range between 0.75 and 4.0 wt% to obtain homogenous films on the different substrates (i.e. Si-wafer, glass slides, gold coated glass slides). Below concentrations of 0.75 wt% CX incompletely covered films were obtained, whereas above 4.0 wt% CX fully covered but inhomogeneous films having a wide range of defects were observed (data not shown). After deposition by spin coating, the solid CX films were regenerated using HCl. When the films were immersed in aqueous 3 M HCl, the films peeled off the substrate; therefore this method was not investigated in further detail. In contrast, the exposure to HCl vapors created from concentrated hydrochloric acid led to the regeneration of the CX to cellulose. The regeneration of the CX can be observed by naked eye, particularly when glass slides were employed as substrates. On these films, the yellow CX film steadily decolorizes with time concomitant with the formation of a white particle like coverage on the surface. This white material corresponds to the formation of salt crystals composed of NaCl (obtained as side product by reaction of the sodium xanthate and HCl) and can be easily removed by rinsing the films with water after regeneration (see AFM section). As expected, the higher the concentration of the CX solution used for film preparation was, the more pronounced the formation of this residue was.

The layer thickness of the CX films gradually increased with increasing CX concentration from 31 ± 3 (0.75 wt%) to 688 ± 10 nm (4.0 wt%). One would expect that the films significantly shrink during regeneration since the xanthate group is rather bulky and hydrogen bond formation would lead additionally to densification of the films as known for related cases involving other cellulose derivatives such as TMSC (Kontturi et al. 2011; Mohan et al. 2012). However, directly after exposure of the films to HCl vapors, the opposite phenomenon was observed. Some films showed an increase in film thickness or did not change significantly which relates to the formation of NaCl, which certainly does not only form on the surface but also in the interior of the film. After removal of NaCl from the films by rinsing with water, the film thickness reduced significantly. Finally, pure cellulose films with film thicknesses between 21 ± 2 and 413 ± 11 nm were manufactured. It is of interest that the film with the smallest layer thickness showed just 23% of shrinking whereas for all the other films shrinkage was between 40 and 44% (Fig. 1). Similar observations have been made when studying the film thickness reduction during regeneration of TMSC; obviously the substrate limits the shrinking and densification of the films to some extent (Ehmann et al. 2015). Afterwards, the films were subjected to a drying procedure at 105 °C over a period of 1 h. The films showed a slight shrinkage whereas here the thinnest films were affected to a larger extent (17% layer thickness decrease) than the thicker ones (few percent decrease). An overview on the layer thickness data is provided in Table 1.

Fig. 1
figure 1

Film thickness changes [%] in dependence of process parameters

Table 1 Comparison of the layer thickness d [nm] of the films depending on initial CX concentration by stylus force profilometry

A commonly used method to monitor chemical reactions of cellulose derivatives is IR spectroscopy. A complication in the IR spectroscopic analysis of the CX films arises from the tendency of CX to decompose at ambient conditions. In the case of CX thin films, the IR spectra indicated that significant amounts of CX decomposition products had been formed after spin coating. The decomposition products can be identified as CS2C=S at 1520 cm−1), sodium sulfide (1420, 920 cm−1) and sodium trithiocarbonate (1670, 1427, 925 and 885 cm−1) and, in traces, H2S (νS–H at 2630 cm−1, Fig. S7, Supporting Information). The remaining bands at 1452 and 1382 cm−1 as well as a weak band at 2725 cm−1 can be assigned to NaOH, which is present in the films stemming from the dissolution of CX (Andrews et al. 1960; Dautzenberg and Philipp 1970; Ogura and Sobue 1968). Since NaOH is highly hygroscopic, the water peak at 1640 cm−1 is pronounced as well. The C–S and C=S vibrations for the CX have been reported in the range between 1050 and 1250 cm−1 and interfere with the decomposition products as well as with vibrations at the pyranose ring, which also show absorption bands in this range. Therefore, an unambiguous assignment is not possible (Fig. 2).

Fig. 2
figure 2

Smoothed ATR-IR spectra of the 600–2000 cm−1 region of cellulose xanthate films (prepared from a 3 wt% solution of CX) during the exposure to HCl vapors. The original spectra for the individual exposure times showing the full range can be found in the Supporting Information (Figs. S7–S10)

During ongoing regeneration, all bands related to C=S and C–S vibrations as well as the NaOH disappear and a cellulose II like spectrum is obtained. It seems that the HCl treatment leads to partial oxidation of the film surface since a weak band at 1762 cm−1 characteristic for C=O vibrations in COOH of oxidized cellulose is observed. Washing the films with water removes the water soluble oxidized cellulose fragments and pure cellulose II films are obtained. This is featured by broad bands from 3600 to 3100 cm−1OH) and from 3000 to 2850 cm−1 (C–H stretching vibrations), a series of small weak bands in the region of 1430–1150 cm−1 (C–O–H bending at 1430 cm−1, C–H deformation at 1372 cm−1, OH in plane deformation at 1330 and at 1200 cm−1), a strong and overlapping bands from 1160 to 950 cm−1 (asym. C–O–C vibration at 1155 cm−1, sym. C–O vibration at 1060 cm−1 and C–O stretching at 1035 cm−1) and a small band at 899 cm−1 (C–O–C valence vibration) (Široký et al. 2010). Additionally, the spectra of the regenerated CX films show a band at 1640 cm−1 indicating adsorbed water.

When the CX thin films are stored over a period of 14 days, hydrolysis reactions take place. The intensity of the IR bands at 885, 920, 1427 and 1670 cm−1 (corresponding to the sodium trithiocarbonate) decrease, while the intensity of the bands corresponding to the major decomposition products of sodium sulfide and trithiocarbonate, namely sodium carbonate and sodium hydroxide become more intense (1770, 1458, 1378 cm−1: Na2CO3, 1452, 1382: NaOH, Fig. S11, Supporting Information) (Ingram and Toms 1957). In addition, the CX partially hydrolyzes as well since the intensity of the bands in the 1000–1250 cm−1 changed as well.

The morphology of the films shows a rather homogeneous surface structure, which is altered by the concentration used for film preparation. In general, an increase of the concentration of CX solutions used for the preparation of the films leads to surfaces with higher RMS roughness (Rq) values. Figures 3 (10 × 10 μm2) and 4 (2 × 2 μm2) depict two different types of films, one prepared from 2.5 wt% (Figs. 3a–d, 4a–d) and the other from 1.5 wt% (Figs. 3e–h, 4e–h) CX solutions. It can be clearly seen that the surfaces feature much higher Rq values for the 2.5 wt% films (6.9 nm) than for the 1.5 wt% ones (3.0 nm). The exposure of the films to HCl vapors leads to a significant increase in roughness to 24 and 28 nm, respectively. However, this increase in roughness is not related to rough cellulose surfaces but to the formation of NaCl particles on the surfaces. As already indicated for the profilometry results, more particles are present on surfaces with increasing CX concentration in solutions prepared for film manufacturing and indeed this can also be observed in the AFM images. When the particles are removed by simply rinsing them with water, Rq values are reduced to their initial values (6.8 and 5.1 nm), a phenomenon which has been observed for cellulose thin films derived from TMSC as well. The films exhibit homogeneous surface morphology and a subsequent drying step slightly alters the structure, thereby further reducing the roughness values. Compared to cellophane, the macroscopic congener, the films do not exhibit a preferred orientation (see Fig. S6 for an AFM image of a cellophane film). This orientation is induced by the drawing of the films in machine direction. As a consequence, the cellophane films show different mechanical properties in machine and cross direction as well as a different hydroexpansion behavior in both directions.

Fig. 3
figure 3

AFM images (10 × 10 μm2) and corresponding RMS roughness of different steps in cellulose thin film processing with a 2.5 wt% (upper row) and a 1.5 wt% (lower row) CX solution. The images show the films directly after spin coating (a, e), after 20 min lasting exposure to HCl atmosphere (b, f), after rinsing with de-ionized water (c, g) and after drying for 1 h at 105 °C (d, h). Z-scale is 100/200/50/50 nm for (ad) and 30/300/30/50 nm for (eh)

Fig. 4
figure 4

AFM images (2 × 2 μm2) and corresponding RMS roughness of different steps in cellulose thin film processing with a 2.5 wt% (upper row) and a 1.5 wt% (lower row) CX solution. The images show the films directly after spin coating (a, e), after 20 min lasting exposure to HCl atmosphere (b, f), after rinsing with de-ionized water (c, g) and after drying for 1 h at 105 °C (d, h). Z-scale is 100/200/50/50 nm for (ad) and 30/150/100/50 nm for (eh)

To validate the chemical reactions and the changes in surface morphology appearing in the different steps of cellulose thin film processing, SEM–EDX measurements were performed. The SEM images reveal flower like structures on the surface of CX films directly after the spin coating step (Fig. 5a), which probably originate from sodium hydroxide. These structures are visible in the AFM images as well but since a smaller area was chosen, they could not be identified as separate phase. Further, the growth of crystallites during CX regeneration (Fig. 5b) is visible, which disappear after rinsing with water (Fig. 5c). EDX spectra clearly show that the crystals in Fig. 5b consist of sodium chloride. For the crystals present on the surface of untreated CX films, EDX spectra (Fig. S3–S5, Supporting Information) show a small sulfur peak in addition to the common sodium and chloride peaks. The additional sulfur peak and the different crystalline structure indicate the presence of two different crystals (sodium hydroxide and probably sodium sulfide).

Fig. 5
figure 5

SEM images displaying the film surface after a spin coating of 2.5 wt% CX solution, b regeneration of CX in HCl vapor and c after rinsing the film with water

An important parameter for the investigation of the surface properties of cellulose thin films is wettability. However, the determination of water contact angles on CX films could not be performed since the films were partially dissolved by water thereby giving no meaningful results while with CH2I2 contact angles ranging from 38° to 60° were obtained. After regeneration, the presence of NaCl particles on the surfaces prevented the measurement of the contact angles for both liquids. After rinsing the films with water, static water contact angles between 27° and 38° were determined. The drying of the films did not change the wettability with both liquids significantly. In general, these cellulose surfaces are a bit more hydrophobic than those prepared from TMSC where usually static water contact angles between 24° and 33° have been reported.

Based on this data, the surface free energies (SFE) of the surfaces were determined according to the Owens–Wendt–Rabel and Kaelble method (Kaelble 1970; Owens and Wendt 1969; Rabel 1971). This method exploits the wettability behavior of two different liquids in order to calculate the total surface free energy as well as the dispersive and polar components. In this paper, water and diiodomethane were chosen as test liquids. Figure 6 illustrates that the surface free energy decreases significantly after rinsing the films with de-ionized water. However, the following heat treatment did not have any substantial impact on the surface free energy. Taking a closer look at the decrease in SFE after the washing step reveals that the polar part of SFE energy stays constant over all process steps and only the disperse part of SFE is slightly changing (Table 2).

Fig. 6
figure 6

Surface free energy of cellulose layers prepared from different concentrations, determined directly after film regeneration, rinsing and heat treatment

Table 2 Static contact angle for viscose thin films in different concentrations and various steps of film production

SPR spectroscopy is one of the methods of choice to investigate protein adsorption on thin films allowing for the evaluation of the dry adsorbed mass (adsorbed mass without coupled water) as well as the adsorption kinetics. Protein adsorption was tested using bovine serum albumin (BSA), a widely accepted marker for nonspecific protein interaction, and fibrinogen (FIB), a serum protein which plays an important role in the blood coagulation cascade (Pallister and Watson 2010). Since the herein investigated cellulose thin films are potential materials for the usage in life science applications, SPR experiments were accomplished at a pH value of 7.4 which corresponds to the pH value of human blood. The adsorption experiments were designed in a way that the films were first equilibrated for 45 min using buffer, followed by injection of the protein solutions over a period of 5 min and finalized by a subsequent rinsing step. At these conditions, the protein amount deposited (Fig. 7) on regenerated and washed films was determined to be 1.11 ± 0.18 and 0.22 ± 0.08 mg m−2 for FIB and BSA, respectively (Mohan et al. 2017). The preferred adsorption behavior for FIB in contrast to BSA is observed for other surfaces as well and can be attributed to the size of FIB. FIB (340 kDa) is a larger protein than BSA (66.5 kDa) and displays a larger contact area hereby increasing the extent of adsorption. Heat treatment of the cellulose films led to a decrease in the change of the SPR angle by the half which corresponds to lower protein adsorption (0.55 ± 0.07 mg m−2 for FIB and 0.11 ± 0.04 mg m−2 for BSA). The reduction in the adsorbed protein amount is accompanied with a change in surface morphology of the thin films since the wettability, another factor potentially playing a role, remains nearly constant after drying. A decrease in roughness minimizes the available surface area therefore leading to reduced interaction. There is not any noteworthy difference in adsorption kinetics (see sensograms, Fig. 7b) for all of the experiments, however it seems that proteins start to desorb even before the rinsing step is initiated (after 10 min) at the heat treated surfaces, whereas on the washed films non-bound material is only removed upon rinsing. This could be also related to the overshooting effect as described recently (Rabe et al. 2011). Different phenomena have been described in literature to explain this effect such as the Vroman effect (competition of high affinity species with low adsorption speed vs low affinity species with high adsorption rate), and the Daly and Wertz effect (change of protein conformation after adsorption). In our case it is obvious that reorientation takes place on the surfaces since both proteins under investigation can adsorb in different conformations.

Fig. 7
figure 7

670 nm sensogram recorded during the adsorption of FIB and BSA on washed and heat treated films (a prepared from 1 wt% CX solutions). b The amount of protein adsorbed on differently treated films, calculated through the change in the SPR angle via the de Feijter equation (De Feijter et al. 1978)

Compared to other cellulosic surfaces, for instance cellulose thin films prepared from TMSC, cellulose films made from viscose offer similar protein adsorption behavior. Depending on the adsorption parameters, e.g. adsorption time, flow rate, ionic strength and others, values in the range of 0.3–0.6 mg BSA·m−2 determined by SPR are reported in literature.


In summary, the manufacturing and properties of cellulose thin films derived from CX have been presented. These films, which may be best described as two-dimensional model systems for viscose based materials, display similar properties as other cellulosic materials in terms of wettability/surface free energy and protein interaction behavior. The use of CX as starting material offers the possibility to prepare thin films in the nanometer range from aqueous solutions in large scale whereas regeneration can be performed via the gas phase. During this reaction, volatile side products are generated and NaCl particles are formed which are easily removed by simply rinsing the films with water. Afterwards, homogenous films are obtained which show a regular but rougher and more porous surface compared to those derived from TMSC for instance. The films show similar behavior towards proteins as other cellulose matrices. In future work, we will focus how these films perform in other areas compared to those derived from other sources such as TMSC.


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Weißl, M., Niegelhell, K., Reishofer, D. et al. Homogeneous cellulose thin films by regeneration of cellulose xanthate: properties and characterization. Cellulose 25, 711–721 (2018).

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  • Cellulose thin films
  • Xanthate
  • Vapor phase hydrolysis
  • Protein adsorption