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Linc00852 from cisplatin-resistant gastric cancer cell-derived exosomes regulates COMMD7 to promote cisplatin resistance of recipient cells through microRNA-514a-5p

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Abstract

Objective

Cisplatin (DDP)-based chemotherapy is commonly referred to as advanced gastric cancer (GC). The purpose of this study was to unravel whether Linc00852 from DDP-resistant tumor cell-derived exosomes (Exos) promotes DDP resistance of GC cells.

Methods

Reverse transcription quantitative polymerase chain reaction was used to detect the expression of Linc00852, miR-514a-5p, COMM domain protein 7 (COMMD7) mRNA, Bax mRNA, and Bcl-2 mRNA. Western blot was used to measure the expression of COMMD7 protein. The IC50 value of DDP is determined by MTT assay. The cell proliferation ability was measured by colony formation test. The apoptosis ability was measured by flow cytometry. The interaction between Linc00852, miR-514a-5p, and COMMD7 was confirmed by luciferase reporter gene assay and RNA pull-down assay. Xenograft tumor model was used to study the effect of Linc00852 on DDP resistance in vivo.

Results

Linc00852 was up-regulated in DDP-resistant GC cells and their secreted exosomes. Down-regulating Linc00852 facilitated the sensitivity of DDP-resistant GC cells to DDP. Linc00852 bound to miR-514a-5p and COMMD7 was a target of miR-514a-5p. Linc00852 could regulate COMMD7 expression via targeting miR-514a-5p. Exosomes from DDP-resistant GC cells enhanced the resistance of recipient GC cells to DDP via exosomal delivery of Linc00852. Depletion of Linc00852 repressed the growth and DDP resistance of GC cells in vivo.

Conclusion

Linc00852 from DDP-resistant tumor cell-derived Exos regulates COMMD7 to promote drug resistance of GC cells through miR-514a-5p.

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References

  • Dai Q, Zhang T, Pan J, Li C. LncRNA UCA1 promotes cisplatin resistance in gastric cancer via recruiting EZH2 and activating PI3K/AKT pathway. J Cancer. 2020;11(13):3882–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Deng Z, Rong Y, Teng Y, Zhuang X, Samykutty A, Mu J, Zhang L, Cao P, Yan J, Miller D, Zhang HG. Exosomes miR-126a released from MDSC induced by DOX treatment promotes lung metastasis. Oncogene. 2017;36(5):639–51.

    Article  CAS  PubMed  Google Scholar 

  • Ghafouri-Fard S, Taheri M. Long non-coding RNA signature in gastric cancer. Exp Mol Pathol. 2020;113:104365.

    Article  CAS  PubMed  Google Scholar 

  • Guggenheim DE, Shah MA. Gastric cancer epidemiology and risk factors. J Surg Oncol. 2013;107(3):230–6.

    Article  PubMed  Google Scholar 

  • Hu W, Li H, Wang S. LncRNA SNHG7 promotes the proliferation of nasopharyngeal carcinoma by miR-514a-5p/ELAVL1 axis. BMC Cancer. 2020;20(1):376.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Karimi P, Islami F, Anandasabapathy S, Freedman ND, Kamangar F. Gastric cancer: descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomark Prev. 2014;23(5):700–13.

    Article  Google Scholar 

  • Lee JK, Park SR, Jung BK, Jeon YK, Lee YS, Kim MK, Kim YG, Jang JY, Kim CW. Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells. PLoS One. 2013;8(12):e84256.

    Article  PubMed  PubMed Central  Google Scholar 

  • Li Q, Wang X, Jiang N, Xie X, Liu N, Liu J, Shen J, Peng T. Exosome-transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma. Cancer Med. 2020a;9(17):6354–66.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li W, Liu R, Wei D, Zhang W, Zhang H, Huang W, Hao L. Circular RNA circ-CCAC1 facilitates adrenocortical carcinoma cell proliferation, migration, and invasion through regulating the miR-514a-5p/C22orf46 Axis. Biomed Res Int. 2020b;2020:3501451.

    PubMed  PubMed Central  Google Scholar 

  • Lin FJ, Lin XD, Xu LY, Zhu SQ. Long noncoding RNA HOXA11-AS modulates the resistance of nasopharyngeal carcinoma cells to cisplatin via miR-454-3p/c-Met. Mol Cells. 2020a;43(10):856–69.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lin H, Zhang L, Zhang C, Liu P. Exosomal MiR-500a-3p promotes cisplatin resistance and stemness via negatively regulating FBXW7 in gastric cancer. J Cell Mol Med. 2020b;24(16):8930–41.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu P, Wang H, Liang Y, Hu A, Xing R, Jiang L, Yi L, Dong J. LINC00852 promotes lung adenocarcinoma spinal metastasis by targeting S100A9. J Cancer. 2018;9(22):4139–49.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lu S, Zhang W, Zhang R, Liu P, Wang Q, Shang Y, Wu M, Donaldson K, Wang Q. Comparison of cellular toxicity caused by ambient ultrafine particles and engineered metal oxide nanoparticles. Part Fibre Toxicol. 2015;12:5.

    Article  PubMed  PubMed Central  Google Scholar 

  • Machlowska J, Baj J, Sitarz M, Maciejewski R, Sitarz R. Gastric cancer: epidemiology, risk factors, classification, genomic characteristics and treatment strategies. Int J Mol Sci. 2020;21(11).

  • Qin X, Yu S, Zhou L, Shi M, Hu Y, Xu X, Shen B, Liu S, Yan D, Feng J. Cisplatin-resistant lung cancer cell-derived exosomes increase cisplatin resistance of recipient cells in exosomal miR-100-5p-dependent manner. Int J Nanomed. 2017;12:3721–33.

    Article  CAS  Google Scholar 

  • Shen Y, Xue C, Li X, Ba L, Gu J, Sun Z, Han Q, Zhao RC. Effects of gastric cancer cell-derived exosomes on the immune regulation of mesenchymal stem cells by the NF-kB signaling pathway. Stem Cells Dev. 2019;28(7):464–76.

    Article  CAS  PubMed  Google Scholar 

  • Shen Q, Zheng J, Wang X, Hu W, Jiang Y, Jiang Y. LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD. Biomed Pharmacother. 2020;126:110016.

    Article  CAS  PubMed  Google Scholar 

  • Smyth EC, Nilsson M, Grabsch HI, van Grieken NC, Lordick F. Gastric cancer. Lancet. 2020;396(10251):635–48.

    Article  CAS  PubMed  Google Scholar 

  • Song Z, Wu Y, Yang J, Yang D, Fang X. Progress in the treatment of advanced gastric cancer. Tumour Biol. 2017;39(7):1010428317714626.

    Article  PubMed  Google Scholar 

  • Sun MY, Xu B, Wu QX, Chen WL, Cai S, Zhang H, Tang QF. Cisplatin-resistant gastric cancer cells promote the chemoresistance of cisplatin-sensitive cells via the exosomal RPS3-mediated PI3K-Akt-cofilin-1 signaling axis. Front Cell Dev Biol. 2021;9:618899.

    Article  PubMed  PubMed Central  Google Scholar 

  • Thomson DW, Dinger ME. Endogenous microRNA sponges: evidence and controversy. Nat Rev Genet. 2016;17(5):272–83.

    Article  CAS  PubMed  Google Scholar 

  • Wang D, Xing N, Yang T, Liu J, Zhao H, He J, Ai Y, Yang J. Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis. Cancer Med. 2020a;9(19):7218–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang X, Yu H, Yu Z, Wang D. Exosomal lncRNA HEIH promotes cisplatin resistance in tongue squamous cell carcinoma via targeting miR-3619–5p/HDGF axis. Acta Histochem. 2020;122(8):151647.

    Article  CAS  PubMed  Google Scholar 

  • Wei L, Sun J, Zhang N, Zheng Y, Wang X, Lv L, Liu J, Xu Y, Shen Y, Yang M. Noncoding RNAs in gastric cancer: implications for drug resistance. Mol Cancer. 2020;19(1):62.

    Article  PubMed  PubMed Central  Google Scholar 

  • Xia X, Wang S, Ni B, Xing S, Cao H, Zhang Z, Yu F, Zhao E, Zhao G. Hypoxic gastric cancer-derived exosomes promote progression and metastasis via MiR-301a-3p/PHD3/HIF-1alpha positive feedback loop. Oncogene. 2020;39(39):6231–44.

    Article  CAS  PubMed  Google Scholar 

  • Xiao S, Zhang M, Liu C, Wang D. MiR-514 attenuates proliferation and increases chemoresistance by targeting ATP binding cassette subfamily in ovarian cancer. Mol Genet Genomics. 2018;293(5):1159–67.

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Leng K, Yao Y, Kang P, Liao G, Han Y, Shi G, Ji D, Huang P, Zheng W, Li Z, Li J, Huang L, Yu L, Zhou Y, Jiang X, Wang H, Li C, Su Z, Tai S, Zhong X, Wang Z, Cui Y. A circular RNA, cholangiocarcinoma-associated circular RNA 1, contributes to cholangiocarcinoma progression, induces angiogenesis, and disrupts vascular endothelial barriers. Hepatology. 2021;73(4):1419–35.

    Article  CAS  PubMed  Google Scholar 

  • Ye Y, Yang S, Han Y, Sun J, Xv L, Wu L, Ming L. HOXD-AS1 confers cisplatin resistance in gastric cancer through epigenetically silencing PDCD4 via recruiting EZH2. Open Biol. 2019;9(9):190068.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yi S, Li G, Sun B. Overexpression of LINC00852 promotes prostate cancer cell proliferation and metastasis. Asia Pac J Clin Oncol. 2020.

  • You N, Li J, Gong Z, Huang X, Wang W, Wang L, Wu K, Zheng L. COMMD7 functions as molecular target in pancreatic ductal adenocarcinoma. Mol Carcinog. 2017a;56(2):607–24.

    Article  CAS  PubMed  Google Scholar 

  • You N, Li J, Huang X, Wu K, Tang Y, Wang L, Li H, Mi N, Zheng L. COMMD7 promotes hepatocellular carcinoma through regulating CXCL10. Biomed Pharmacother. 2017b;88:653–7.

    Article  CAS  PubMed  Google Scholar 

  • Zheng L, You N, Huang X, Gu H, Wu K, Mi N, Li J. COMMD7 regulates NF-kappaB signaling pathway in hepatocellular carcinoma stem-like cells. Mol Ther Oncolytics. 2019;12:112–23.

    Article  CAS  PubMed  Google Scholar 

  • Zhou C, Chen Z, Peng C, Chen C, Li H. Long noncoding RNA TRIM52-AS1 sponges miR-514a-5p to facilitate hepatocellular carcinoma progression through increasing MRPS18A. Cancer Biother Radiopharm. 2021;36(2):211–9.

    CAS  PubMed  Google Scholar 

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Funding

The Natural Science Foundation program of Zhejiang Province Y18H030042.

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Authors and Affiliations

  1. Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, Zhejiang, China

    Shuguang Cao, Beilei Fu, Dingli Zhang, Chenxing Wang & Hao Wu

  2. Department of Comprehensive Medicine, Wenzhou Central Hospital Medical Group, the Affiliated Second Hospital of Shanghai University, Affiliated Dingli Clinical Institute of Wenzhou Medical University, Wenzhou, 325000, China

    Jing Cai

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  1. Shuguang Cao
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  2. Beilei Fu
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  3. Jing Cai
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  4. Dingli Zhang
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  5. Chenxing Wang
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  6. Hao Wu
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Contributions

HW contributed to study design; SC and BF contributed to manuscript editing; JC contributed to experimental studies; DZ and CW contributed to data analysis. All authors read and approved the final manuscript.

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Correspondence to Hao Wu.

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The authors declare no competing interests.

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Shuguang Cao and Beilei Fu are co-first authors.

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10565_2021_9685_MOESM1_ESM.jpg

Supplementary Figure 1. Linc00852 loaded by GC cells-derived exosomes acts as a ceRNA of miR-514a-5p to promote COMMD7 expression and enhance DDP resistance of GC cells. (JPG 771 KB)

Supplementary file2 (DOCX 18 KB)

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Cao, S., Fu, B., Cai, J. et al. Linc00852 from cisplatin-resistant gastric cancer cell-derived exosomes regulates COMMD7 to promote cisplatin resistance of recipient cells through microRNA-514a-5p. Cell Biol Toxicol 39, 483–496 (2023). https://doi.org/10.1007/s10565-021-09685-y

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