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Protective effects of β-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes

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Abstract

β-Glucans (BGs) are polysaccharides that are found in the cell walls of organisms such as bacteria, fungi, and some cereals. The objective of the present study was to investigate the genotoxic and antigenotoxic effects of BG extracted from the mushroom Agaricus brasiliensis (= Agaricus blazei Murrill ss. Heinemann). The mutagenic activity of BG was tested in single-cell gel electrophoresis assays with human peripheral lymphocytes. In addition, the protective effects against the cooked food mutagen 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and (+/−)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), which is the main metabolite of B[a]P, and against ROS (H2O2)-induced DNA damage, were studied. The results showed that the compound itself was devoid of mutagenic activity, and that a significant dose-dependent protective effect against damage induced by hydrogen peroxide and Trp-P-2 occurred in the dose range 20–80 μg/ml. To investigate the prevention of Trp-P-2-induced DNA damage, a binding assay was carried out to determine whether BG inactivates the amine via direct binding. Since no such interactions were observed, it is likely that BG interacts with enzymes involved in the metabolism of the amine.

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Abbreviations

PBS:

phosphate-buffered saline

FTIR:

Fourier transform infrared

HPLC:

high-performance liquid chromatography

SCGE:

single-cell gel electrophoresis

HA:

heterocyclic aromatic amine

BG:

β-glucan

BPDE:

(+/−)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide

(Trp-P-2):

3-amino-1-methyl-5H-pyrido[4,3-b]indole

ROS:

reactive oxygen species

References

  • Bellini MF, Giacomini NL, Eira AF, Ribeiro LR, Mantovani MS. Anticlastogenic effect of aqueous extracts of Agaricus blazei on CHO-k1 cells, studying different developmental phases of the mushroom. Toxicol In Vitro. 2003,17:465–9.

    Article  PubMed  CAS  Google Scholar 

  • Bellini MF, Angeli JPF, Matuo R., Terezan, AP, Ribeiro LR., Mantovani MS. Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells. Toxicol In Vitro. 2006;20:355–60.

    Article  PubMed  CAS  Google Scholar 

  • Dashwood RH. Modulation of heterocyclic amine-induced mutagenicity and carcinogenicity: an ‘A-to-Z’ guide to chemopreventive agents, promoters and transgenic models. Mutat Res. 2002;511: 89–112.

    Article  PubMed  CAS  Google Scholar 

  • Delmanto RD, De Lima PLA, Sugui MM, et al. Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide. Mutat Res. 2001;496:15–21.

    PubMed  CAS  Google Scholar 

  • Ferguson LR, Roberton AM, Watson ME, Kestell P, Harris PJ. The adsorption of a range of dietary carcinogens by alpha-cellulose, a model insoluble dietary fiber. Mutat Res. 1993;319:257–66.

    Article  PubMed  CAS  Google Scholar 

  • Gonzaga MLC, Ricardo NMPS, Heatley F, Soares S de A. Isolation and characterization of polysaccharides from Agaricus blazei Murill. Carbohydr Polym. 2005a;60: 3–49.

    Article  Google Scholar 

  • Gonzaga MLC, Menezes TMF, Ricardo NMPS, Sandra AS. Isolation, characterization and evaluation of the capacity of solubilization of drugs of isolated polysaccharides from mushroom Agaricus blazei Murill. Presented at the 8th Brazilian Polymer Congress, águas de Lindóia, São Paulo. Proceedings in CD Rom. 2005b.

  • Guterrez ZR, Mantovani MS, Eira AF, Ribeiro LR, Jordao BQ. Variation of the antimutagenicity effects of water extracts of Agaricus blazei Murrill in vitro. Toxicol In Vitro. 2004;18:301–9.

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto T, Nonaka Y, Minato K, et al. Suppressive effect of polysaccharides from the edible and medicinal mushrooms, Lentinus edodes and Agaricus blazei, on the expression of cytochrome P450s in mice. Biosci Biotechnol Biochem. 2002;66:1610–1614. auq (11) Should pages be 1610–4 or 610–4?

    Google Scholar 

  • Helma C, Uhl M. A public domain image-analysis program for the single-cell gel electrophoresis (comet) assay. Mutat Res. 2000;466:9–15.

    PubMed  CAS  Google Scholar 

  • Lazarova M, Labaj J, Kovacikova Z, Slamenova D. Diet containing fungal (1→3)-β-glucan derivative exhibits protective effects against DNA lesions induced in freshly isolated rat cells. Neoplasma. 2004;51:431–5.

    PubMed  CAS  Google Scholar 

  • Lindl T, Bauer J. 1993. Zell- und Gewebekultur. Stuttgart, Gustav Fischer Verlag, Jena, New York. auq (12) What is the publisher's name?

    Google Scholar 

  • Luiz RC, Jordão BQ, Eira AF, Ribeiro LR, Mantovani MS. Mechanism of anticlastogenicity of Agaricus blazei Murrill mushroom organic extracts in wild type CHO(k1) and repair deficient (xrs5) cells by chromosome aberration and sister chromatid exchange assays. Mutat Res. 2003a;528:75–9.

    CAS  Google Scholar 

  • Luiz RC, Jordão BQ, Eira AF, Ribeiro LR, Mantovani MS. Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells. Cytologia. 2003b;68:1–6.

    Article  Google Scholar 

  • Menoli RCN, Mantovani MS, Ribeiro LR, Gunter S, Jordão BQ. Antimutagenic effects of the mushroom Agaricus blazei Murill extracts on V79 cells. Mutat Res. 2001;496:5–13.

    PubMed  CAS  Google Scholar 

  • Mizuno T. Bioactive biomolecules of mushrooms–food, function and medicinal effect of mushroom fungi. Food Rev Int. 1995;11(1):7–21.

    CAS  Google Scholar 

  • Muchove JJ and Muchove RMC. Noções básicas de microbiologia. Minas Gerais: Editora Folha de Viçosa; 1989.

    Google Scholar 

  • Okamoto T, Kodoi R, Nonaka Y, et al. Lentinan from shiitake mushroom (Lentinus edodes) suppresses expression of cytochrome P450 1A subfamily in the mouse liver. Biofactors. 2004;21:407–9.

    PubMed  CAS  Google Scholar 

  • Oliveira JM, Jordão BQ, Ribeiro LR, Eira AF, Mantovani MS. Anti-genotoxic effect of aqueous extracts of sun mushroom (Agaricus blazei Murill lineage 99/26) in mammalian cells in vitro. Food Chem Toxicol. 2002;40:15–20.

    Google Scholar 

  • Reynolds JA, Kastello MD, Harrigton DG, et al. Glucan-induced enhancement of host resistance to selected infectious diseases. Infect Immun. 1980;30:51–7.

    PubMed  CAS  Google Scholar 

  • Schwab CE, Huber WW, Parzefall W, et al. Search for compounds that inhibit the genotoxic and carcinogenic effects of heterocyclic aromatic amines. Crit Rev Toxicol. 2000;30:1–69.

    Article  PubMed  CAS  Google Scholar 

  • Singh NP, McCoy MT, Tice RR, Ishidate M Jr, Schneider EL. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175:184–191.

    Article  PubMed  CAS  Google Scholar 

  • Speit G, Hartmann A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage. Methods Mol Biol. 2005;291:85–95.

    PubMed  CAS  Google Scholar 

  • Steinkellner H, Rabot S, Freywald C, et al. Effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutat Res. 2001;480:285–7.

    PubMed  Google Scholar 

  • Tohamy AA, El-Ghor AA, El-Nahas SM, Noshy MM. β-Glucan inhibits the genotoxicity of cyclophosphamide, adriamycin and cisplatin. Mutat Res. 2003;541:45–53.

    PubMed  CAS  Google Scholar 

  • Tzianabos AO and Cisneros L. Prophylaxis with the immunomodulator PGG glucan enhances antibiotic efficacy in rats infected with antibiotic-resistant bacteria. Ann NY Acad Sci. 1996;797:285–287.

    PubMed  CAS  Google Scholar 

  • Williams GM, Williams CM, Weisburger JH. Diet and cancer prevention: the fiber first diet. Toxicol Sci. 1999;52:72–86.

    PubMed  CAS  Google Scholar 

  • Yoshioka Y, Tabeta R, Saitó H, Uehara N, Fukuoka F. Antitumor polysaccharides from P. ostreatus (Fr.) Quel.: isolation and structure of a beta-glucan. Carbohydr Res. 1985;140:93– 100.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil

    J. P. F Angeli & M. S. Mantovani

  2. Departamento de Microbiologia, Universidade Estadual de Londrina, Londrina, PR, Brazil

    R. E. Linhares

  3. Programa de Pós-Graduação em Biologia Celular e Molecular, Depto. de Biologia, UNESP, Rio Claro, SP, Brazil

    L. R. Ribeiro

  4. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, Brazil

    M. L. C. Gonzaga, S. de A. Soares & M. P. S. N. Ricardo

  5. Departamento de Biologia Geral, UNESP, SP, Assis, Brazil

    M. S. Tsuboy

  6. Institute of Analytical Chemistry and Food Chemistry, University of Vienna, Vienna, Austria

    R. Stidl

  7. Institute of Cancer Research, Medical University of Vienna, Vienna, Austria

    S. Knasmueller

  8. Departamento de Biologia Geral – CCB, Universidade Estadual de Londrina – Campus Universitário, Cx. Postal 6001, Londrina, Paraná, Brazil, 86051-990

    M. S. Mantovani

Authors
  1. J. P. F Angeli
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  2. L. R. Ribeiro
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  3. M. L. C. Gonzaga
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  4. S. de A. Soares
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  5. M. P. S. N. Ricardo
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  6. M. S. Tsuboy
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  7. R. Stidl
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  8. S. Knasmueller
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  9. R. E. Linhares
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  10. M. S. Mantovani
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Correspondence to M. S. Mantovani.

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Angeli, J.P.F., Ribeiro, L.R., Gonzaga, M.L.C. et al. Protective effects of β-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes. Cell Biol Toxicol 22, 285–291 (2006). https://doi.org/10.1007/s10565-006-0087-z

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  • DOI: https://doi.org/10.1007/s10565-006-0087-z

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