Abstract
This study succeeded in the isolation of potent laccase endophytic fungus producer Alternaria arborescens MK629314 from medicinal plants collected from Wadi Abu Matir, Saint- Catherine-Protectorate, South Sinai-Egypt. Full physiochemical and thermodynamic characterization for A. arborescens MK629314 laccase was investigated showing the highest activity at a temperature of 50 °C and pH 5. The kinetics constants, Km (Michael's constant) and Vmax (maximum velocity) were determined to be 4 mM and 133.33 U.ml−1, respectively. Also, the thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 15.13 and 48.85 kJ.mol−1, respectively. Thermal denaturation parameters as Kd (denaturation rate constant), T1/2 (half-life), D-values (decimal reduction time), ΔH° (enthalpy), ΔG° (Gibbs free energy), and ΔS° (entropy) were determined. The pretreatment of olive mill wastewater (OMWW) 10 and 20% with A.arborescens MK629314 caused, the maximum polyphenols removal of 63.06 and 27.29%, respectively. A.arborescens MK629314 of 50% acetone fraction succeeded in saccharification of five agricultural wastes with the highest reducing sugar recovery from cantaloupe peel which was improved by central composite design to be 240.90 mg.ml−1. A. arborescens MK629314 and its produced laccase proved to be a biological sufficient tool for the detoxification of wastewater from the olive oil industry and delignification of wastes from agriculture.
Graphical Abstract
Phylogenetic relationship of potent endophytic fungi for laccase production with related fungal strains based on internal transcribed spacer sequences from NCBI GenBank.
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Abd El Aty, A.A., Zohair, M.M. & Mostafa, F.A. Characterization, and Application Studies on Alternaria arborescens MK629314 Laccase. Catal Lett 153, 1574–1586 (2023). https://doi.org/10.1007/s10562-022-04120-1
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DOI: https://doi.org/10.1007/s10562-022-04120-1