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Gelatin-Immobilized Manganese Peroxidase with Novel Catalytic Characteristics and Its Industrial Exploitation for Fruit Juice Clarification Purposes

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Abstract

In the present study, glutaraldehyde (GLA) activated gelatin hydrogel was used as a solid support to encapsulate the manganese peroxidase (MnP; E.C. 1.11.1.13) produced by Ganoderma lucidum IBL-05 under pre-optimized growth environment. Through gelatin-assisted immobilization, a maximal of 83.2 ± 2.91 % immobilization yield was achieved at optimum conditions of gelatin; 20.0 % (w/v), GLA 0.25 % (v/v) after 2 h activation time using 0.6 mg/mL of enzyme concentration. In contrast to aqueous form, the insolubilized MnP presented its maximum activity at pH 6.0 and 60 °C. Inevitably, enzyme immobilization significantly (P < 0.05) increased the thermal stability profile of in-house isolated MnP. At 60 °C, maximum activity of free MnP decreased to 14.2 ± 1.4 %, whereas immobilized MnP retained 70.18 ± 3.2 % of its original activity after 120 min. To explore the industrial applicability of MnP, the immobilized MnP was tested for apple and orange fruit juice clarification features in a packed bed reactor system. The immobilized MnP showed commendable results in the de-bittering’s of investigated fruit juices, decreasing 42.7 % of the original apple juice color and 36.3 of its turbidity. Whereas, the color and turbidity reduction characteristics of orange juice were 51.5 and 43.6 %, respectively. After six consecutive cycles, the immobilized-MnP was able to retain more than 60.0 % of its initial activity. Collectively, catalytic, thermo-stability and clarity amelioration features of the gel-entrapped MnP suggest a high potential of enzymatic treatment for biotechnological exploitability.

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Acknowledgments

The financial support provided by the Higher Education Commission (HEC), Islamabad, Pakistan is thankfully acknowledged. The authors are also grateful to the State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China for providing technical and analytical help.

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Authors and Affiliations

  1. Industrial Biotechnology Laboratory, Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan

    Muhammad Bilal & Muhammad Asgher

  2. State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Muhammad Bilal, Hongbo Hu & Xuehong Zhang

  3. School of Engineering and Science, Tecnologico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501, CP 64849, Monterrey, N.L., Mexico

    Hafiz M. N. Iqbal

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  1. Muhammad Bilal
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  2. Muhammad Asgher
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  3. Hafiz M. N. Iqbal
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  4. Hongbo Hu
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  5. Xuehong Zhang
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Correspondence to Hafiz M. N. Iqbal.

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Bilal, M., Asgher, M., Iqbal, H.M.N. et al. Gelatin-Immobilized Manganese Peroxidase with Novel Catalytic Characteristics and Its Industrial Exploitation for Fruit Juice Clarification Purposes. Catal Lett 146, 2221–2228 (2016). https://doi.org/10.1007/s10562-016-1848-9

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  • DOI: https://doi.org/10.1007/s10562-016-1848-9

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