Abstract
The use of fresh amniotic membrane (AM) is not a viable option, as it has many disadvantages. Preserving the AM reduces the risk of cross-infection and maintains its effectiveness for a long time. In order to maximize the therapeutic effects of the AM, the basic need is to preserve its vitality and the bioactive molecules it contains. However, the effect of preservation procedures on cell viability and growth factors is a still matter of debate. Optimum preservation method is expected to be cost-effective, easily-accessible, and most importantly, to preserve the effectiveness of the tissue for the longest time. However, each preservation technique has its advantages and disadvantages over the other, and each one compromises the vitality and bioactive molecules of the tissue to some extent. Therefore, the best method of preservation is still controversial, and the question of ‘how to preserve the AM best?’ has not yet been definitively answered.
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MG, IK and OBS were responsible for the conception or design of the work. MG was responsible for the preparing figures. MG and IK were responsible for drafting the article. OBS was responsible for the critical revision of the article. All authors were responsible for the final approval of the version to be published.
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Gurdal, M., Korkmaz, I. & Barut Selver, O. An important detail that is still not clear in amniotic membrane applications: How do we store the amniotic membrane best?. Cell Tissue Bank 25, 339–347 (2024). https://doi.org/10.1007/s10561-023-10121-0
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DOI: https://doi.org/10.1007/s10561-023-10121-0