Abstract
To determine the expression of signal transducer and activator of transcription 3 (STAT3) in patients with fragility fractures (FFs) and its effect on the biological function of osteoblasts. The study included 32 patients with FFs who were diagnosed and treated in the research group and 30 concurrent healthy individuals in the control group. We observed STAT3 mRNA expression in the patients with FFs and controls and altered STAT3 mRNA to detect changes in the proliferation, invasion, and apoptosis of osteoblasts. The patients with FFs presented higher serum STAT3 mRNA expression than the controls (P < 0.05). We plotted receiver operating characteristic curves based on the STAT3 mRNA expression and found that the area under the curve for STAT3 mRNA was 0.856 (P < 0.05). Transfection of STAT3 mRNA mimics resulted in increased STAT3 mRNA expression, inhibited cell proliferation as detected by an MTT assay, and increased apoptosis rate, which was determined using flow cytometry with human fetal osteoblastic cell line 1.19 cells. STAT3 mRNA expression was elevated in the serum of patients with FFs and can be used as a biomarker for the diagnosis of the disease. Regulating STAT3 mRNA can inhibit the proliferation and induce the osteoblasts apoptosis.
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The data used to support the findings of this study are available from the corresponding author upon request.
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Funding
This work was supported by the project of Jiangsu Health and Family Planning Commission: Role and mechanism of STAT3 regulatory T cell pathway in fracture healing (Project No.: H2018024).
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GS, JL and YT conceived and designed the research and interpreted the results of experiments. GS, JL, ZW, XZ and YT performed experiments, analyzed data. GS and JL wrote the manuscript.
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The study was approved by the Medical Ethics Committee of Jinling Hospital, Medicine College, Nanjing University.
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Sun, G., Lu, J., Wu, Z. et al. STAT3 expression in patients with fragility fractures and its effect on the biological function of osteoblasts. Cell Tissue Bank 24, 515–522 (2023). https://doi.org/10.1007/s10561-022-10051-3
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DOI: https://doi.org/10.1007/s10561-022-10051-3