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Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro

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Abstract

Objectives

This study assessed possible osteogenic differentiation caused by electromagnetic fields (EMF) on rat bone-marrow-derived stem cells (rBMSCs) cultured in osteogenic medium (OM) or in human adipose-stem cell-conditioned medium (hADSC-CM).

Materials and Methods

The rBMSCs were divided into negative and positive control groups, cultured in α-MEM plus 10% FBS or OM respectively. CM and CM + EMF  groups, cultured cells in hADSCs-CM or exposed to EMF (50 Hz, 1 mT) for 30 min/day plus hADSCs-CM, respectively. Cells from the OM + EMF were simultaneously cultured in OM and exposed to EMF. Osteogenesis was investigated through alkaline phosphatase activity, alizarin red staining and real-time PCR.

Results

A meaningfully higher level of ALP activity was observed in the OM + EMF group compared to the other groups. There was a considerable increase in Runx2 expression in the CM + EMF group compared to the positive control and CM groups and a significant increase in Runx2 expression in the OM + EMF in comparison with all other groups after 21 days. Runx2 expression increased significantly in the CM, CM + EMF and positive control groups on day 21 compared to the same groups on day 14. From days 14–21, Ocn expression increased in the CM and CM + EMF groups, but both groups showed a significant decrease compared to the positive controls. CM and EMF had no effect on Ocn expression. On day 21, Ocn expression was significantly higher in the OM + EMF group than in the positive control group.

Conclusion

The synergistic effect of EMF and OM increased the expression of Runx2 and Ocn in rBMSCs.

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Acknowledgements

This study was financially supported by the Biological School of Damghan University.

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Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Damghan University, Damghan, Iran

    Fateme Amirahmadi

  2. Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran

    Maryam Haji Ghasem Kashani & Meysam Nasiri

  3. Department of Physical Chemistry, School of Chemistry, Damghan University, Damghan, Iran

    Seyyed Ahmad Nabavi Amri

  4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Vahideh Assadollahi

  5. Department of Bioinformatics and Genomics, University of North Carolina Charlotte, Kannapolis, NC, USA

    Azita Alasvand Zarasvand

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  1. Fateme Amirahmadi
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  2. Maryam Haji Ghasem Kashani
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Correspondence to Maryam Haji Ghasem Kashani.

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Amirahmadi, F., Haji Ghasem Kashani, M., Nasiri, M. et al. Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro. Cell Tissue Bank 24, 317–328 (2023). https://doi.org/10.1007/s10561-022-10034-4

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