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Effect of different cryopreservation regimens on Ehrlich carcinoma growth

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Abstract

The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells. The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites. The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days. Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined. Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found. Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used. Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells. None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells. The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.

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Funding

This work was supported by funding from the National Academy of Sciences of Ukraine (No. 2.2.6.110).

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Authors and Affiliations

  1. Department of Cryopathophysiology and Immunology, Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine

    A. M. Goltsev, M. O. Bondarovych, N. M. Babenko, Yu. O. Gaevska, T. G. Dubrava & M. V. Ostankov

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  1. A. M. Goltsev
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  2. M. O. Bondarovych
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  3. N. M. Babenko
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  4. Yu. O. Gaevska
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  5. T. G. Dubrava
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  6. M. V. Ostankov
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Correspondence to A. M. Goltsev.

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The research was performed in accordance with the Law of Ukraine “On the Protection of Animals Against Cruelty” (No. 3447-IV, 2006), with meeting the requirements of the Institute’s Bioethics Committee, which are in accordance with the provisions of the “European Convention on the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes” (Strasbourg 1986).

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Goltsev, A.M., Bondarovych, M.O., Babenko, N.M. et al. Effect of different cryopreservation regimens on Ehrlich carcinoma growth. Cell Tissue Bank 20, 411–421 (2019). https://doi.org/10.1007/s10561-019-09780-9

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  • DOI: https://doi.org/10.1007/s10561-019-09780-9

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