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Effect of storage temperature on gentamicin release from antibiotic-coated bone chips

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Abstract

Freezing is the most common method for storing bones until use in skeletal reconstruction. However, the effect of freezing on antibiotic delivery from antibiotic-coated bone has not been evaluated. In this study, we compared antibiotic delivery in vitro from gentamicin-coated human bone stored at different temperatures. Bone chips obtained from human femur heads were chemically cleaned and mixed with gentamicin sulfate. Samples were stored for 4 months at −20 °C, 4 months at −80 °C, or evaluated immediately without freezing. Antibiotic release from the bone chips was measured using Bacillus subtilis as an indicator strain. Zones of inhibition and rates of gentamicin release were similar in all three groups. Storage at −20 and −80 °C for bone allografts has no effect on gentamicin release from chemically cleaned bone chips.

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Acknowledgments

We thank Mag. Dennis Huber for technical and statistical assistance and David Putzer, MSc. for help with data collection. The authors declare no competing interests. This study was sponsored by the Department of Experimental Orthopaedics, Medical University Innsbruck.

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Authors and Affiliations

  1. Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University Innsbruck, Salurnerstrasse 15, 6020, Innsbruck, Austria

    D. C. Coraça-Huber & M. Nogler

  2. Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Innsbruck, Austria

    J. Hausdorfer & M. Fille

Authors
  1. D. C. Coraça-Huber
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  2. J. Hausdorfer
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  3. M. Fille
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  4. M. Nogler
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Correspondence to D. C. Coraça-Huber.

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Coraça-Huber, D.C., Hausdorfer, J., Fille, M. et al. Effect of storage temperature on gentamicin release from antibiotic-coated bone chips. Cell Tissue Bank 14, 395–400 (2013). https://doi.org/10.1007/s10561-012-9339-8

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  • DOI: https://doi.org/10.1007/s10561-012-9339-8

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