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The influence of various storage conditions on cell viability in amniotic membrane

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Abstract

Up to now freeze-dried, gamma-sterilised or glycerol-preserved amniotic membranes (AMs) have widely been used in the field of ophthalmology and wound care (e.g. leg ulcers, burns). After some preservation processes in use, like freeze-drying or glycerol-preserving, the cells in the AM are no longer viable. Within this study we evaluated the influence of different short-term and long-term storage conditions on cell viability in AM. Therefore AMs from cesarean section placentae were washed and biopsied to evaluate the microbiological status and to determine the viability of the tissue. Additionally, viability under various storage conditions was examined by assessment of mitochondrial activity. Preservation included temperatures above and below 0°C as well as various media compositions. As expected, cell viability in amnion decreases during storage, in fact the effect was more pronounced when stored frozen, but the higher viability of amnion obtained by storage above 0°C with medium is associated with the limitation to a short period of storage of about 28 days. The evaluated preservation methods are the basis for future non-clinical in-vivo studies in which the possible benefit of amnion as a viable biomaterial in wound healing will be investigated.

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Abbreviations

AM(s):

Amniotic membrane(s)

DMEM:

Dulbecco’s modified eagle medium

DMSO:

Dimethylsulfoxide

FCS:

Fetal calf serum

Ham’s F12:

Ham’s F12-medium

HBV:

Hepatitis B virus

HCV:

Hepatitis C virus

HIV:

Human immunodeficiency virus

HSA:

Human serum albumin

L15:

Leibovitz’s L15-medium

N2 :

Liquid nitrogen

PBS:

Phosphate buffered saline

RPMI:

RPMI 1640-medium

RT:

Room temperature

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Acknowledgements

We wish to thank the Women’s Hospital of Upper Austria ‘Landes-Frauen- und Kinderklink Linz’ for their assistance in amniotic membrane procurement.

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Authors and Affiliations

  1. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Linz-Vienna, Austria

    Simone Hennerbichler, Bernd Reichl, Daniela Pleiner & Heinz Redl

  2. Red Cross Transfusion Service of Upper Austria, Linz, Austria

    Christian Gabriel

  3. Bio-Products & Bio-Engineering AG, Vienna, Austria

    Simone Hennerbichler, Bernd Reichl & Johann Eibl

  4. Blumauerstrasse 3-5, 4020, Linz, Austria

    Simone Hennerbichler

Authors
  1. Simone Hennerbichler
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  2. Bernd Reichl
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  3. Daniela Pleiner
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  4. Christian Gabriel
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  5. Johann Eibl
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  6. Heinz Redl
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Corresponding author

Correspondence to Simone Hennerbichler.

Additional information

A part of this work was presented at the World Congress on Tissue Banking in Rio in May 2005 and was honoured by the Poster Award Commission.

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Hennerbichler, S., Reichl, B., Pleiner, D. et al. The influence of various storage conditions on cell viability in amniotic membrane. Cell Tissue Banking 8, 1–8 (2007). https://doi.org/10.1007/s10561-006-9002-3

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  • DOI: https://doi.org/10.1007/s10561-006-9002-3

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