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Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model

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Abstract

Purpose

Aim of the study was to evaluate the therapeutic potential of endothelial cell seeding to inhibit the neointimal response after balloon injury of the rat carotid artery.

Methods and results

Endothelial cells were isolated from peripheral blood after cytokine-stimulation (PB-EC), the bone marrow compartment (BM-EC), and the thoracic aorta (AO-EC) of male rats. The EC-populations were characterized by microscopy, cytofluorometry, and PCR-analysis, and displayed distinct patterns of RNA-expression of the EC-markers VEGF-receptor 1 and 2, and TIE2. 5 × 105 ECs were incubated in the freshly balloon-denuded arterial bed for 30 min achieving about 70% coverage with all 3 EC-populations. However, the neointimal response 2 weeks after the procedure was significantly aggravated in the EC-seeded carotids (I/M-ratio: non-seeded Control 1.00 ± 0.10, PB-EC 1.53 ± 0.07, BM-EC 1.64 ± 0.12, and AO-EC 1.55 ± 0.14; P < 0.05 vs. Control for all). We found evidence for an accelerated cell-turnover (TUNEL-assay, total cell-density, infiltration with CD45pos haematopoietic cells) especially in the adventitia of the treated vessels.

Conclusion

Endothelial cell seeding fails to prevent intimal hyperplasia following arterial injury in the rat carotid model.

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Acknowledgement

The authors want to thank G. Pietrzyk and T. Liebeld for their excellent technical assistance, and M. Rodriguez-Gomez, Ph.D. from the Mack-lab for his help with the cytofluorometric tests.

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Authors and Affiliations

  1. Department of Internal Medicine II, Regensburg University Medical Center, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany

    Bertram J. Jobst, Guenter A. J. Riegger & Daniel P. Griese

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  1. Bertram J. Jobst
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  2. Guenter A. J. Riegger
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  3. Daniel P. Griese
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Correspondence to Daniel P. Griese.

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Jobst, B.J., Riegger, G.A.J. & Griese, D.P. Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model. Cardiovasc Drugs Ther 23, 343–353 (2009). https://doi.org/10.1007/s10557-009-6191-6

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  • DOI: https://doi.org/10.1007/s10557-009-6191-6

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