Graphene is a two-dimensional carbon nanomaterial. It consists of a planar film composed of carbon atoms with sp2 hybrid orbitals, which is hexagonal and honeycomb in shape. The thickness of graphene is only 0.34 nm, and the unique two-dimensional lattice and electron structure of graphene also makes it possess excellent physical and chemical properties. When the traditional carbon material cannot detect some substances with very similar oxidation potential or some at ultra-trace level, the emergence of graphene replaces the traditional carbon material and provides the possibility for the preparation of biochemical sensors that can measure the above substances, which has a very broad development prospect. Based on the excellent properties of graphene, this paper starts from four common biochemical substances in daily life, including explosives, pesticides, pathogens and toxins and introduces the principle and effects of various biochemical sensors based on graphene and its composites for these four substances. Finally, the future development trend is prospected.
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References
Guo C X, Li C M. A self-assembled hierarchical nanostructure comprising carbon spheres and graphene nanosheets for enhanced supercapacitor performance [J]. Energy & Environmental Science, 2011, 4(11): 4504-4507.
Pumera M. Graphene-based nano materials for energy storage [J]. Energy&Environmental Science, 2011, 4(3):668-674.
Brownson DAC, Kampouris DK, Banks CE. An overview of graphene in energy production and storage applications [J]. Journal of Power Sources, 2011, 196(11):4873-4885.
Shang N G, Papakonstantinou P, McMullan M, et al. Catalyst-free efficient growth, orientation and biosensing properties of multilayer graphene nanoflake films with sharp edge planes [J]. Advanced functional materials, 2008, 18(21): 3506-3514.
Novoselov K S, Geim A K, Morozov S V, et al. Electric field effect in atomically thin carbon films [J]. Science, 2004, 306(5696): 666-669.
Novoselov K S, Geim A K, Morozov S V, et al. Two-dimensional gas of massless Dirac fermions in graphene [J]. Nature, 2005, 438(7065): 197-200.
Morozov S V, Novoselov K S, Katsnelson M I, et al. Giant intrinsic carrier mobilities in graphene and its bilayer [J]. Physical review letters, 2008, 100(1): 016602.
Balandin A A, Ghosh S, Bao W, et al. Superior thermal conductivity of single-layer graphene [J]. Nano letters, 2008, 8(3): 902-907.
Margine E R, Bocquet M L, Blase X. Thermal stability of graphene and nanotube covalent functionalization [J]. Nano letters, 2008, 8(10): 3315-3319.
Lee C, Wei X, Kysar JW, etal. Measurement of the elastic properties and intrinsic strength of monolayer graphene [J]. science, 2008, 321(5887):385-388.
Ding R, Li W, Wang X, et al. A brief review of corrosion protective films and coatings based on graphene and graphene oxide [J]. Journal of Alloys and Compounds, 2018, 764: 1039-1055.
Lu Shu-hua, Wang Yin-wen. Developments in [J]. Spectroscopy and Spectral Analysis, 2018, 38(05):1412-1419.
Guo L, Yang Z, Dou X. Artificial olfactory system for trace identification of explosive vapors realized by optoelectronic Schottky sensing [J]. Advanced materials (Deerfield Beach Fla.), 2017, 29(5).
Tang L, Feng H, Cheng J, et al. Uniform and rich-wrinkled electrophoretic deposited graphene film: a robust electrochemical platform for TNT sensing [J]. Chemical communications, 2010, 46(32): 5882-5884.
Li J, Kuang D, Feng Y, et al. A graphene oxide-based electrochemical sensor for sensitive determination of 4-nitrophenol [J]. Journal of hazardous materials, 2012, 201: 250-259.
Goh M S, Pumera M. Graphene-based electrochemical sensor for detection of 2, 4, 6-trinitrotoluene (TNT) in seawater: the comparison of single-, few-, and multilayer graphene nanoribbons and graphite microparticles [J]. Analytical and bioanalytical chemistry, 2011, 399(1): 127-131.
Li H, Mehler W T, Lydy M J, et al. Occurrence and distribution of sediment-associated insecticides in urban waterways in the Pearl River Delta, China [J]. Chemosphere, 2011, 82(10): 1373-1379.
Wu Xiaoxiao, Mei Xiuming, Jiang Diyao, Xu Jingjing, Zhang Chi. Progress on Application of Carbon Nanomaterials in Sample Pretreatment for Organophosphorus Pesticide Detection [J]. Food Science and Technology, 2022, 47(03):318-323.
Handbook of carbon nano materials [M]. World Scientific, 2012.
Cremisini C, Di Sario S, Mela J, et al. Evaluation of the use of free and immobilised acetylcholinesterase for paraoxon detection with an amperometric choline oxidase based biosensor [J]. Analytica Chimica Acta, 1995, 311(3): 273-280.
Vidal J C, Esteban S, Gil J, et al. A comparative study of immobilization methods of a tyrosinase enzyme on electrodes and their application to the detection of dichlorvos organophosphorus insecticide [J]. Talanta, 2006, 68(3): 791-799.
Liu T, Xu M, Yin H, et al. A glassy carbon electrode modified with graphene and tyrosinase immobilized on platinum nanoparticles for sensing organophosphorus pesticides [J]. Microchimica Acta, 2011, 175(1): 129-135.
Gong J, Miao X, Zhou T, et al. An enzymeless organophosphate pesticide sensor using Au nanoparticle-decorated graphene hybrid nanosheet as solid-phase extraction [J]. Talanta, 2011, 85(3): 1344-1349.
Wang Y, Zhang S, Du D, et al. Self assembly of acetylcholinesterase on a gold nanoparticles–graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker [J]. Journal of Materials Chemistry, 2011, 21(14): 5319-5325.
Zhang L, Zhang A, Du D, et al. Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticides [J]. Nanoscale, 2012, 4(15): 4674-4679.
Darvishnejad F, Raoof J B, Ghani M. MIL-101 (Cr)@ graphene oxide-reinforced hollow fiber solid-phase microextraction coupled with high-performance liquid chromatography to determine diazinon and chlorpyrifos in tomato, cucumber and agricultural water [J]. Analytica Chimica Acta, 2020, 1140: 99-110.
Jung J H, Cheon D S, Liu F, et al. A graphene oxide based immuno-biosensor for pathogen detection [J]. Angewandte Chemie, 2010, 122(33): 5844-5847.
Robinson J T, Perkins F K, Snow E S, et al. Reduced graphene oxide molecular sensors [J]. Nano letters, 2008, 8(10): 3137-3140.
Huang Y, Dong X, Liu Y, et al. Graphene-based biosensors for detection of bacteria and their metabolic activities [J]. Journal of Materials Chemistry, 2011, 21(33): 12358-12362.
Wan Y, Lin Z, Zhang D, et al. Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria [J]. Biosensors and Bioelectronics, 2011, 26(5): 1959-1964.
Ono T, Kanai Y, Inoue K, et al. Electrical biosensing at physiological ionic strength using graphene field-effect transistor in femtoliter microdroplet [J]. Nano Letters, 2019, 19(6): 4004-4009.
Liu Xiaobo, Kou Zongkui, Mu Shichun. Porous Graphene Materials [J]. Progress in Chemistry ,2015,27(11):1566.
Shi J J, Zhu J J. Sonoelectrochemical fabrication of Pd-graphene nanocomposite and its application in the determination of chlorophenols [J]. Electrochimica acta, 2011, 56(17): 6008-6013.
Busca G, Berardinelli S, Resini C, et al. Technologies for the removal of phenol from fluid streams: a short review of recent developments [J]. Journal of hazardous materials, 2008, 160(2-3): 265-288.
Kim T H, Lee B Y, Jaworski J, et al. Selective and sensitive TNT sensors using biomimetic polydiacetylene-coated CNTFETs [J]. ACS nano, 2011, 5(4): 2824-2830.
Snow E S, Perkins F K, Houser E J, et al. Chemical detection with a single-walled carbon nanotube capacitor [J]. Science, 2005, 307(5717): 1942-1945.
Robinson J A, Snow E S, Perkins F K. Improved chemical detection using single-walled carbon nanotube network capacitors [J]. Sensors and Actuators A: Physical, 2007, 135(2): 309-314.
Kong L, Wang J, Fu X, et al. p-Hexafluoroisopropanol phenyl covalently functionalized single-walled carbon nanotubes for detection of nerve agents [J]. Carbon, 2010, 48(4): 1262-1270.
Kuang Z, Kim S N, Crookes-Goodson W J, et al. Biomimetic chemosensor: designing peptide recognition elements for surface functionalization of carbon nanotube field effect transistors [J]. ACS nano, 2010, 4(1): 452-458.
Park M, Cella L N, Chen W, et al. Carbon nanotubes-based chemiresistive immunosensor for small molecules: Detection of nitroaromatic explosives [J]. Biosensors and Bioelectronics, 2010, 26(4): 1297-1301.
Robinson J T, Perkins F K, Snow E S, et al. Reduced graphene oxide molecular sensors [J]. Nano letters, 2008, 8(10): 3137-3140.
Wu C, Sun D, Li Q, et al. Electrochemical sensor for toxic ractopamine and clenbuterol based on the enhancement effect of graphene oxide [J]. Sensors and Actuators B: Chemical, 2012, 168: 178-184.
Shen Youming, Nie Jiyun, Li Zhixia, Li Haifei, Wu Yonglong, Zhang Jianyi. Progress in Research on Mycotoxins Contamination, Toxicity, Biosynthesis and Regulatory Factors of Mycotoxins in Fruits [J]. Food Science,2018,39(09):294-304.
Hauhan R, Singh J, Sachdev T, et al. Recent advances in mycotoxins detection [J]. Biosensors and Bioelectronics, 2016, 81: 532-545.
Ma Haihua, Zhang Yuan, Zhen Tong, Sun Jizhou, Xia Shanhong. Recent developments and applications of Electrochemical Biosensors for Aflatoxins Detection [J]. Journal of the Chinese Cereals and Oils Association 2016,31(02):132-140.
Wang Qi, Yang Qingli, Wu Wei. A Graphene Oxide-Based Fluorescent Aptasensor for Determination of Mycotoxins in Foods [J]. Food Science,2021,42(24):318-322.
Srivastava S, Abraham S, Singh C, et al. Protein conjugated carboxylated gold@ reduced graphene oxide for aflatoxin B1 detection [J]. RSC Advances, 2015, 5(7): 5406-5414.
Yin Long-jing, Qiao Jia-bin, He Lin. Structures and Electronic properties of Twisted Bilayer Graphene [J]. Progress in Physics,2016,36(03):65-99.
Ye R, James D K, Tour J M. Laser-induced graphene [J]. Accounts of chemical research, 2018, 51(7): 1609-1620.
Dosi M, Lau I, Zhuang Y, et al. Ultrasensitive electrochemical methane sensors based on solid polymer electrolyte-infused laser-induced graphene [J]. ACS applied materials & interfaces, 2019, 11(6): 6166-6173.
Chhetry A, Sharifuzzaman M, Yoon H, et al. MoS2-decorated laser-induced graphene for a highly sensitive, hysteresisfree, and reliable piezoresistive strain sensor [J]. ACS applied materials & interfaces, 2019, 11(25): 22531-22542.
Stanford M G, Yang K, Chyan Y, et al. Laser-induced graphene for flexible and embeddable gas sensors [J]. ACS nano, 2019, 13(3): 3474-3482.
Yuan W, Shi G. Graphene-based gas sensors [J]. Journal of Materials Chemistry A, 2013, 1(35): 10078-10091.
Nag A, Mukhopadhyay S C, Kosel J. Sensing system for salinity testing using laser-induced graphene sensors [J]. Sensors and Actuators A: Physical, 2017, 264: 107-116.
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Translated from Khimiya i Tekhnologiya Topliv i Masel, No. 4, pp. 108–113 July –August, 2022.
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Wang, Y., He, W. Biochemical Sensors Based on Graphene and Its Composites. Chem Technol Fuels Oils 58, 717–724 (2022). https://doi.org/10.1007/s10553-022-01439-8
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DOI: https://doi.org/10.1007/s10553-022-01439-8