MSCs and inflammation: new insights into the potential association between ALCL and breast implants

Abstract

Possible association between anaplastic large cell lymphoma (ALCL) and breast implants has been suggested. In this context, formation of the periprosthetic capsule has been reported as a cause of inflammation, which plays a key role in tumor onset. Tumors take advantage of inflammation to influence and interfere with the host immune response by secreting multiple factors, and their onset and survival is in turn affected by the paracrine effects from mesenchymal stem cells (MSCs). In this study, we tried to clarify how inflammation can modify the immunobiology and the exerted paracrine effect of MSCs. MSCs derived from both inflamed (I-MSCs) and control (C-MSCs) tissues were isolated and co-cultured with an ALCL cell line. Proliferation rate and the expression of selected cytokines were tested. I-MSCs secrete higher levels of cytokine related to chronic inflammation than C-MSCs. After co-cultures with KI-JK cells, C- and I-MSCs show the same variation in the cytokine expression, with an increase of IL2, IL4, IL5, IL10, IL13, TNF-α, TGF-β, and G-CSF. Proliferation of ALCL cells was not influenced by co-cultures. Our results state that (i) inflamed microenvironment affects the immunobiology of MSCs modifying the profile of the expressed cytokines, and (ii) the paracrine effects exerted by MSCs on ALCL cells are not influenced by inflammation. Moreover, it seems that ALCL cells are able to manipulate MSCs’ immunoregulatory properties to evade the host immune control. Nevertheless, this ability is not associated with inflammation and the question about BIA-ALCL is not proved by our experiments.

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References

  1. 1.

    Hu T, Li LF, Shen J, Zhang L, Cho CH (2015) Chronic inflammation and colorectal cancer: the role of vascular endothelial growth factor. Curr Pharm Des 21:2960–2967

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Raposo TP, Beirão BC, Pang LY, Queiroga FL, Argyle DJ (2015) Inflammation and cancer: till death tears them apart. Vet J 205:161–174

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Chai EZ, Siveen KS, Shanmugam MK, Arfuso F, Sethi G (2015) Analysis of the intricate relationship between chronic inflammation and cancer. Biochem J 468:1–15

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Orciani M, Lazzarini R, Scartozzi M, Bolletta E, Mattioli-Belmonte M, Scalise A, Di Benedetto G, Di Primio R (2013) The response of breast cancer cells to mesenchymal stem cells: a possible role of inflammation by breast implants. Plast Reconstr Surg 132:899e–910e

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Orciani M, Bolletta E, Campanati A, Di Benedetto G, Di Primio R (2014) The response of breast cancer cells to mesenchymal stem cells: a possible role of inflammation by breast implants. Plast Reconstr Surg 134:994e–996e

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Mariotti C, Lazzarini R, Nicolai M, Saitta A, Orsini E, Orciani M, Di Primio R (2015) Comparative study between amniotic-fluid mesenchymal stem cells and retinal pigmented epithelium (RPE) stem cells ability to differentiate towards RPE cells. Cell Tissue Res 362:21–31

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Orciani M, Davis S, Appolloni G, Lazzarini R, Mattioli-Belmonte M, Ricciuti RA, Boscaro M, Di Primio R, Arnaldi G (2015) Isolation and characterization of progenitor mesenchymal cells in human pituitary tumors. Cancer Gene Ther 22:9–16

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Orciani M, Campanati A, Salvolini E, Lucarini G, Di Benedetto G, Offidani A, Di Primio R (2011) The mesenchymal stem cell profile in psoriasis. Br J Dermatol 165:585–592

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Orciani M, Morabito C, Emanuelli M, Guarnieri S, Sartini D, Giannubilo SR, Di Primio R, Tranquilli AL, Mariggiò MA (2011) Neurogenic potential of mesenchymal-like stem cells from human amniotic fluid: the influence of extracellular growth factors. J Biol Regul Homeost Agents 25:115–130

    CAS  PubMed  Google Scholar 

  10. 10.

    Orciani M, Mariggiò MA, Morabito C, Di Benedetto G, Di Primio R (2010) Functional characterization of calcium-signaling pathways of human skin-derived mesenchymal stem cells. Skin Pharmacol Physiol. 23:124–132

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Fierro FA, Sierralta WD, Epuñan MJ, Minguell JJ (2004) Marrow-derived mesenchymal stem cells: role in epithelial tumor cell determination. Clin Exp Metastasis 21:313–319

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Sasser AK, Mundy BL, Smith KM, Studebaker AW, Axel AE, Haidet AM, Fernandez SA, Hall BM (2007) Human bone marrow stromal cells enhance breast cancer cell growth rates in a cell line-dependent manner when evaluated in 3D tumor environments. Cancer Lett 254:255–264

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Klopp AH, Gupta A, Spaeth E, Andreeff M, Marini F 3rd (2011) Concise review: dissecting a discrepancy in the literature: do mesenchymal stem cells support or suppress tumor growth? Stem Cells 29:11–19 (Review)

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Lee YS, Filie A, Arthur D, Fojo AT, Jaffe ES (2015) Breast-implant-associated anaplastic large cell lymphoma in a patient with Li-Fraumeni syndrome. Histopathology 67:925–927

    Article  PubMed  Google Scholar 

  15. 15.

    Locke MB, Lofts J (2015) Variable presentation of anaplastic large-cell lymphoma in patients with breast implants. ANZ J Surg 1:2–6

  16. 16.

    Hwang MJ, Brown H, Murrin R, Momtahan N, Sterne GD (2015) Breast implant-associated anaplastic large cell lymphoma: a case report and literature review. Aesthetic Plast Surg 39:391–395

    Article  PubMed  Google Scholar 

  17. 17.

    Kim B, Predmore ZS, Mattke S, van Busum K, Gidengil CA (2015) Breast implant-associated anaplastic large cell lymphoma: updated results from a structured expert consultation process. Plast Reconstr Surg Glob Open 3:e296

    Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Peters W (2014) Update on anaplastic large cell lymphoma in women with breast implants. Can J Plast Surg 22:267–269

    Google Scholar 

  19. 19.

    Gidengil CA, Predmore Z, Mattke S, van Busum K, Kim B (2015) Breast implant-associated anaplastic large cell lymphoma: a systematic review. Plast Reconstr Surg 135:713–720

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Brody GS, Deapen D, Taylor CR, Pinter-Brown L, House-Lightner SR, Andersen JS, Carlson G, Lechner MG, Epstein AL (2015) Anaplastic large cell lymphoma occurring in women with breast implants: analysis of 173 cases. Plast Reconstr Surg 135:695–705

    CAS  Article  PubMed  Google Scholar 

  21. 21.

    Adrada BE, Miranda RN, Rauch GM, Arribas E, Kanagal-Shamanna R, Clemens MW, Fanale M, Haideri N, Mustafa E, Larrinaga J, Reisman NR, Jaso J, You MJ, Young KH, Medeiros LJ, Yang W (2014) Breast implant-associated anaplastic large cell lymphoma: sensitivity, specificity, and findings of imaging studies in 44 patients. Breast Cancer Res Treat 147:1–14

    CAS  Article  PubMed  Google Scholar 

  22. 22.

    Xu J, Wei S (2014) Breast implant-associated anaplastic large cell lymphoma: review of a distinct clinicopathologic entity. Arch Pathol Lab Med 138:842–846

    Article  PubMed  Google Scholar 

  23. 23.

    Miranda RN, Aladily TN, Prince HM, Kanagal-Shamanna R, de Jong D, Fayad LE et al (2014) Breast implant-associated anaplastic large-cell lymphoma: long-term follow-up of 60 patients. J Clin Oncol 32:114–120

    Article  PubMed  PubMed Central  Google Scholar 

  24. 24.

    Campanati A, Orciani M, Consales V, Lazzarini R, Ganzetti G, Di Benedetto G, Di Primio R, Offidani A (2014) Characterization and profiling of immunomodulatory genes in resident mesenchymal stem cells reflect the Th1-Th17/Th2 imbalance of psoriasis. Arch Dermatol Res 306:915–920

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Campanati A, Orciani M, Gorbi S, Regoli F, Di Primio R, Offidani A (2012) Effect of biologic therapies targeting tumour necrosis factor-α on cutaneous mesenchymal stem cells in psoriasis. Br J Dermatol 167:68–76

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Orciani M, Di Primio R (2013) Skin-derived mesenchymal stem cells: isolation, culture, and characterization. Methods Mol Biol 989:275–283

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Orciani M, Gorbi S, Benedetti M, Di Benedetto G, Mattioli-Belmonte M, Regoli F, Di Primio R (2010) Oxidative stress defense in human-skin-derived mesenchymal stem cells versus human keratinocytes: different mechanisms of protection and cell selection. Free Radic Biol Med 49:830–838

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Kim B, Roth C, Chung KC, Young VL, van Busum K, Schnyer C, Mattke S (2011) Anaplastic large cell lymphoma and breast implants: a systematic review. Plast Reconstr Surg 127:2141–2150

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Lazzarini R, Olivieri F, Ferretti C, Mattioli-Belmonte M, Di Primio R, Orciani M (2014) mRNAs and miRNAs profiling of mesenchymal stem cells derived from amniotic fluid and skin: the double face of the coin. Cell Tissue Res 355:121–130

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Coussens LM, Werb Z (2002) Inflammation and cancer. Nature 420:860–867

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  31. 31.

    Elinav E, Nowarski R, Thaiss CA, Hu B, Jin C, Flavell RA (2013) Inflammation-induced cancer: crosstalk between tumours, immune cells and microorganisms. Nat Rev Cancer 13:759–771

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Balkwill F, Mantovani A (2001) Inflammation and cancer: back to Virchow? Lancet 357:539–545

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Ono M (2008) Molecular links between tumor angiogenesis and inflammation: inflammatory stimuli of macrophages and cancer cells as targets for therapeutic strategy. Cancer Sci 99:1501–1506

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Balkwill F, Charles KA, Mantovani A (2005) Smoldering and polarized inflammation in the initiation and promotion of malignant disease. Cancer Cell 7:211–217

    CAS  Article  PubMed  Google Scholar 

  35. 35.

    de Visser KE, Coussens LM (2006) The inflammatory tumor microenvironment and its impact on cancer development. Contrib Microbiol 13:118–137

    Article  PubMed  Google Scholar 

  36. 36.

    Motz GT, Coukos G (2013) Deciphering and reversing tumor immune suppression. Immunity 39:61–73

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  37. 37.

    Aggarwal BB (2009) Inflammation, a silent killer in cancer is not so silent! Curr Opin Pharmacol 9:347–350

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Aggarwal BB, Gehlot P (2009) Inflammation and cancer: how friendly is the relationship for cancer patients? Curr Opin Pharmacol 9:351–369

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  39. 39.

    Grivennikov SI, Greten FR, Karin M (2010) Immunity, inflammation, and cancer. Cell 140:883–899

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  40. 40.

    Hu H, Jacombs A, Vickery K, Merten SL, Pennington DG, Deva AK (2015) Chronic biofilm infection in breast implants is associated with an increased T-cell lymphocytic infiltrate: implications for breast implant-associated lymphoma. Plast Reconstr Surg 135:319–329

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    George EV, Pharm J, Houston C, Al-Quran S, Brian G, Dong H, Hai W, Reeves W, Yang LJ (2013) Breast implant-associated ALK-negative anaplastic large cell lymphoma: a case report and discussion of possible pathogenesis. Int J Clin Exp Pathol 6:1631–1642

    PubMed  PubMed Central  Google Scholar 

  42. 42.

    Kopf M, Baumann H, Freer G, Freudenberg M, Lamers M, Kishimoto T, Zinkernagel R, Bleuthmann H, Kohler G (1994) Impaired immune and acute-phase responses in interleukin-6-deficient mice. Nature 368:339–342

    CAS  Article  PubMed  Google Scholar 

  43. 43.

    Romani L, Mencacci A, Cenci E, Spaccapelo R, Toniatti C, Puccetti P, Bistoni F, Poli V (1996) Impaired neutrophil response and CD41 T helper cell 1 development in interleukin-6-deficient mice infected with Candida albicans. J Exp Med 183:1345–1355

    CAS  Article  PubMed  Google Scholar 

  44. 44.

    Ruzek MC, Miller AH, Opal SM, Pearce BD, Biron CA (1997) Characterization of early cytokine responses and an interleukin-6-dependent pathway of endogenous glucocorticoid induction during murine cytomegalovirus infection. J Exp Med 185:1185–1192

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Kaplanski G, Marin V, Montero-Julian F, Mantovani A, Farnarier C (2003) IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol 24:25–29

    CAS  Article  PubMed  Google Scholar 

  46. 46.

    Lin WW, Karin M (2007) A cytokine-mediated link between innate immunity, inflammation, and cancer. J Clin Invest 117:1175–1183

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  47. 47.

    Smyth MJ, Dunn GP, Schreiber RD (2006) Cancer immunosurveillance and immunoediting: the roles of immunity in suppressing tumor development and shaping tumor immunogenicity. Adv Immunol 90:1–50

    CAS  Article  PubMed  Google Scholar 

  48. 48.

    Bui JD, Schreiber RD (2007) Cancer immunosurveillance, immunoediting and inflammation: independent or interdependent processes? Curr Opin Immunol 19:203–208

    CAS  Article  PubMed  Google Scholar 

  49. 49.

    Teng MW, Swann JB, Koebel CM, Schreiber RD, Smyth MJ (2008) Immune-mediated dormancy: an equilibrium with cancer. J Leukoc Biol 84:988–993

    CAS  Article  PubMed  Google Scholar 

  50. 50.

    Swann JB, Vesely MD, Silva A, Sharkey J, Akira S, Schreiber RD, Smyth MJ (2008) Demonstration of inflammation-induced cancer and cancer immunoediting during primary tumorigenesis. Proc Natl Acad Sci USA 105:652–656

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  51. 51.

    Swann JB, Smyth MJ (2007) Immune surveillance of tumors. J Clin Invest 117:1137–1146

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  52. 52.

    Siegel G, Schäfer R, Dazzi F (2009) The immunosuppressive properties of mesenchymal stem cells. Transplantation 87:S45–S49

    Article  PubMed  Google Scholar 

  53. 53.

    Lu H, Ouyang W, Huang C (2006) Inflammation, a key event in cancer development. Mol Cancer Res 4:221–233

    Article  PubMed  Google Scholar 

  54. 54.

    Steinbrink K, Jonuleit H, Müller G, Schuler G, Knop J, Enk AH (1999) Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8+ T cells resulting in a failure to lyse tumor cells. Blood 93:1634–1642

    CAS  PubMed  Google Scholar 

  55. 55.

    Bayne L, Beatty GL, Jhala N, Clark CE, Rhim AD, Stanger BZ, Vonderheide RH (2012) Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer. Cancer Cell 21:822–835

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  56. 56.

    Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol 9:162–174

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This work was supported by the Grants FIRB-RBAP1153LS_004 and PRIN 201098WFZ2_006 from Ministero dell’Istruzione, dell’Università e della Ricerca, Rome, Italy.

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Correspondence to Roberto Di Primio.

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None of the authors have a financial interest in any of the products, devices, or drugs mentioned in this manuscript.

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The study and the patient enrollment were approved by the Marche Polytechnic University Ethical Commitee and were conducted in accordance with the Declaration of Helsinki.

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Orciani, M., Sorgentoni, G., Torresetti, M. et al. MSCs and inflammation: new insights into the potential association between ALCL and breast implants. Breast Cancer Res Treat 156, 65–72 (2016). https://doi.org/10.1007/s10549-016-3745-8

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Keywords

  • ALCL
  • MSCs
  • Breast implants
  • Paracrine effects