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Skin-Derived Mesenchymal Stem Cells: Isolation, Culture, and Characterization

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 989))

Abstract

The increasing interest about stem cell research is linked to the promise of developing such treatments for many life-threatening, debilitating diseases and for cell replacement therapies. Among the various human tissues, skin represents a source characterized by great accessibility and availability with noninvasive procedures and without risks of oncogenesis after their transplantation. In this aim, the identification of suitable protocols for the isolation, characterization, and long-term storage has a pivotal role.

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References

  1. Odorico JS, Kaufman DS, Thomson JA (2001) Multilineage differentiation from human embryonic stem cell lines. Stem Cells 19:193–204

    Article  PubMed  CAS  Google Scholar 

  2. Schuldiner M, Eiges R, Eden A et al (2001) Induced neuronal differentiation of human embryonic stem cells. Brain Res 913:201–205

    Article  PubMed  CAS  Google Scholar 

  3. Wakitani S, Takaoka K, Hattori T et al (2003) Embryonic stem cells injected into the mouse knee joint form teratomas and subsequently destroy the joint. Rheumatology (Oxford) 42:162–165

    Article  CAS  Google Scholar 

  4. Pittenger MF, Mackay AM, Beck SC et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147

    Article  PubMed  CAS  Google Scholar 

  5. Zvaifler NJ, Marinova-Mutafchieva L, Adams G et al (2000) Mesenchymal precursor cells in the blood of normal individuals. Arthritis Res 2:477–488

    Article  PubMed  CAS  Google Scholar 

  6. in’t Anker PS, Scherjon SA, Kleijburg-van der Keur C et al (2003) Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood 102:1548–1549

    Article  Google Scholar 

  7. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295

    Article  PubMed  CAS  Google Scholar 

  8. Campagnoli C, Roberts IA, Kumar S et al (2001) Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood 98:2396–2402

    Article  PubMed  CAS  Google Scholar 

  9. Orciani M, Morabito C, Emanuelli M et al (2011) Neurogenic potential of mesenchymal-like stem cells from human amniotic fluid: the influence of extracellular growth factors. J Biol Regul Homeost Agents 25:15–30

    Google Scholar 

  10. Orciani M, Trubiani O, Vignini A et al (2009) Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells. Acta Histochem 111:15–24

    Article  PubMed  CAS  Google Scholar 

  11. Bartsch G, Yoo JJ, De Coppi P et al (2005) Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. Stem Cells Dev 14:337–348

    Article  PubMed  CAS  Google Scholar 

  12. Baker DE, Harrison NJ, Maltby E et al (2007) Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Nat Biotechnol 25:207–215

    Article  PubMed  CAS  Google Scholar 

  13. Jekabsons K, Riekstina U, Parfejevs V et al (2011) Culture-expanded human dermal stem cells exhibit donor to donor differences in cAMP generation. Cell Tissue Res 345:253–263

    Article  PubMed  CAS  Google Scholar 

  14. Orciani M, Gorbi S, Benedetti M et al (2010) Oxidative stress defense in human-skin-derived mesenchymal stem cells versus human keratinocytes: different mechanisms of protection and cell selection. Free Radic Biol Med 1:830–838

    Article  Google Scholar 

  15. Orciani M, Mariggiò MA, Morabito C et al (2010) Functional characterization of calcium-signaling pathways of human skin-derived mesenchymal stem cells. Skin Pharmacol Physiol 23:124–132

    Article  PubMed  CAS  Google Scholar 

  16. Guan L, Yu J, Zhong L et al (2011) Biological safety of human skin-derived stem cells after long-term in vitro culture. J Tissue Eng Regen Med 5:97–103

    Article  PubMed  CAS  Google Scholar 

  17. Ernst N, Tiede S, Tronnier V et al (2010) An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin  +  progenitors from adult human dermis. Exp Dermatol 19:549–555

    Article  PubMed  Google Scholar 

  18. Orciani M, Emanuelli M, Martino C et al (2008) Potential role of culture mediums for successful isolation and neuronal differentiation of amniotic fluid stem cells. Int J Immunopathol Pharmacol 21:595–602

    PubMed  CAS  Google Scholar 

  19. Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

This work was supported by grant FIRB–RBAP10MLK7_003 from Ministero dell’Istruzione, dell’Universita e della Ricerca, Rome, Italy.

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Orciani, M., Di Primio, R. (2013). Skin-Derived Mesenchymal Stem Cells: Isolation, Culture, and Characterization. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_21

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  • DOI: https://doi.org/10.1007/978-1-62703-330-5_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-329-9

  • Online ISBN: 978-1-62703-330-5

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