Skip to main content

Advertisement

SpringerLink
Log in

Irreversible electroporation for the treatment of rabbit VX2 breast cancer

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

Irreversible electroporation (IRE) is considered predominantly as a non-thermal ablative technique that uses electrical fields to permeabilize cell membranes and lead to cell death. In this study, we evaluated the efficacy of IRE in the rabbit VX2 breast cancer model. Thirty-five female New Zealand white rabbits were inoculated against VX2 breast cancer cells. The rabbits were randomly divided into two groups: a control group of 15 rabbits and an IRE treatment group of 20 rabbits. Treatment and treatment outcome were evaluated by computerized tomography (CT) scan (plain or contrast enhanced), tumor growth curves and pathological examination including H&E, TUNEL, PCNA and CD31 staining. All rabbits in the IRE treatment group experienced successful IRE without obvious complications except for thoracic major muscle injury. A focused, complete and well-defined ablation zone where tumor cells have been thoroughly eradicated was detected by H&E staining, along with increasing TUNEL staining. The expression of PCNA and CD31 was down-regulated at the periphery of the ablation region. As of the last follow-up, 10 rabbits (67%) in IRE group demonstrated disease is under control; 2 rabbits (13%) are in stable condition; 3 rabbits (20%) suffered from disease progression; the remaining 5 rabbits were sacrificed for pathological examination halfway through the study. Overall, the efficacy of IRE was demonstrated by the creation of a complete ablation region, with increased apoptosis in the ablation zone and decreased proliferation and microvessel density of tumor tissue at the periphery. IRE is a promising local treatment for breast cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • O. Akinwande, S.S. Ahmad, T. Van Meter, B. Schulz, R.C. Martin, J. Oncol. (2015). doi:10.1155/2015/680319

    Google Scholar 

  • B. Al-Sakere, F. André, C. Bernat, E. Connault, P. Opolon, R.V. Davalos, B. Rubinsky, L.M. Mir, PLoS One 2, e1135 (2007)

    Article  Google Scholar 

  • S.S. Bhatia, R. Arya, G. Narayanan, Tech. Vasc. Interv. Radiol. 18, 170 (2015)

    Article  Google Scholar 

  • T. Boehm, A. Malich, S.N. Goldberg, J.R. Reichenbach, I. Hilger, P. Hauff, M. Reinhardt, M. Fleck, W.A. Kaiser, Radiology 222, 805 (2002)

    Article  Google Scholar 

  • D.R. Brenin, Ann. Surg. Oncol. 18, 3088 (2011)

    Article  Google Scholar 

  • W. Cao, X. Xu, J. Zhang, Y. Duan, PLoS One 8, e61583 (2013)

    Article  Google Scholar 

  • X. Chen, Z. Ren, T. Zhu, X. Zhang, Z. Peng, H. Xie, L. Zhou, S. Yin, J. Sun, S. Zheng, Sci. Report. 5, 16233 (2015)

    Article  Google Scholar 

  • Z. Cole-Healy, P. Vergani, K. Hunter, N.J. Brown, M.W. Reed, C.A. Staton, Exp. Mol. Pathol. 99, 19 (2015)

    Article  Google Scholar 

  • M.V. de Oliveira, E.P. Pereira Gomes, C.S. Pereira, L.R. de Souza, L.O. Barros, D.C. Mendes, A.L. Guimarães, A.M. De Paula, Appl. Immunohistochem. Mol. Morphol. 21, 444 (2013)

    Article  Google Scholar 

  • K. El Gehani, L. Al-Kikhia, N. Mansuri, K. Syrjanen, O. Al-Fituri, A. Elzagheid, Libyan J. Med. 6 (2011). doi:10.3402/ljm.v6i0.6016

  • G.M. Fadda, D.A. Santeufemia, S.M. Basso, R. Tozzoli, F. Falcomer, F. Lumachi, Med. Chem. 12, 280 (2016)

    Article  Google Scholar 

  • H. Furusawa, K. Namba, H. Nakahara, C. Tanaka, Y. Yasuda, E. Hirabara, M. Imahariyama, K. Komaki, Breast Cancer 14, 55 (2007)

    Article  Google Scholar 

  • R.E. Gangnon, B.L. Sprague, N.K. Stout, O. Alagoz, H. Weedon-Fekjaer, T.R. Holford, A. Trentham-Dietz, Cancer Epidemiol. Biomark. Prev. 24, 905 (2015)

    Article  Google Scholar 

  • D. Groheux, M. Espie, S. Giacchetti, E. Hindie, Radiology 266, 388 (2013)

    Article  Google Scholar 

  • B.A. Grotenhuis, W.W. Vrijland, T.M. Klem, Eur. J. Surg. Oncol. 39, 1317 (2013)

    Article  Google Scholar 

  • Y. Guo, Y. Zhang, R. Klein, G.M. Nijm, A.V. Sahakian, R.A. Omary, G.Y. Yang, A.C. Larson, Cancer Res. 70, 1555 (2010)

    Article  Google Scholar 

  • A. Hamza, S. Elrefaey, Gland Surg 3, 149 (2014)

    Google Scholar 

  • T. Jiang, X. Zhang, J. Ding, B. Duan, S. Lu, Int. J. Clin. Exp. Pathol. 8, 13945 (2015)

    Google Scholar 

  • M. Karuturi, N. VanderWalde, H. Muss, Clin. Geriatr. Med. 32, 133 (2016)

    Article  Google Scholar 

  • S. Ke, X.M. Ding, J. Kong, J. Gao, S.H. Wang, Y. Cheng, W.B. Sun, J. Transl. Med. 8, 73 (2010)

    Article  Google Scholar 

  • B. Kos, P. Voigt, D. Miklavcic, M. Moche, Radiol. Oncol. 26, 1652 (2015)

    Google Scholar 

  • E. Lanza, J. Palussiere, X. Buy, R.F. Grasso, B. Beomonte Zobel, D. Poretti, V. Pedicini, L. Balzarini, R.L. Cazzato, J. Vasc. Interv. Radiol. 26, 1652 (2015)

    Article  Google Scholar 

  • E.W. Lee, C. Chen, V.E. Prieto, S.M. Dry, C.T. Loh, S.T. Kee, Radiology 255, 426 (2010)

    Article  Google Scholar 

  • E.W. Lee, D. Wong, B.A. Tafti, V. Prieto, M. Totonchy, J. Hilton, S. Dry, S. Cho, C.T. Loh, S.T. Kee, J. Vasc. Interv. Radiol. 14, 238 (2012)

    Google Scholar 

  • S. Li, F. Chen, L. Shen, Q. Zeng, P. Wu, J. Transl. Med. 14, 238 (2016)

    Article  Google Scholar 

  • G. Manenti, A.L. Scarano, C.A. Pistolese, T. Perretta, E. Bonanno, A. Orlandi, G. Simonetti, Breast Care 8, 356 (2013)

    Article  Google Scholar 

  • L. Mangiavini, E. Schipani, Methods Mol. Biol. 1130, 245 (2014)

    Article  Google Scholar 

  • R.C. Martin, D. Kwon, S. Chalikonda, M. Sellers, E. Kotz, C. Scoggins, K.M. McMasters, K. Watkins, Ann. Surg. 262, 486 (2015)

    Article  Google Scholar 

  • Y. Miyata, K. Mitsunari, A. Asai, K. Takehara, Y. Mochizuki, H. Sakai, Prostate 75, 84 (2015)

    Article  Google Scholar 

  • N.J. Nadkarni, K.D. Geest, T. Neff, B.D. Young, D.P. Bender, A. Ahmed, B.J. Smith, A. Button, M.J. Goodheart, Cancer Lett. 331, 99 (2013)

    Article  Google Scholar 

  • R.E. Neal, R.V. Davalos, Ann. Biomed. Eng. 37, 2615 (2009)

    Article  Google Scholar 

  • R.E. Neal, R. Singh, H.C. Hatcher, N.D. Kock, S.V. Torti, R.V. Davalos, Breast Cancer Res. Treat. 123, 295 (2010)

    Article  Google Scholar 

  • T. Nguyen, E. Hattery, V.P. Khatri, Gland Surg 3, 128 (2014)

    Google Scholar 

  • M. Noguchi, A. Motoyoshi, M. Earashi, H. Fujii, Eur. J. Surg. Oncol. 38, 1036 (2012)

    Article  Google Scholar 

  • J. Palussiere, C. Henriques, L. Mauriac, M. Asad-Syed, F. Valentin, V. Brouste, S. Mathoulin-Pelissier, C. Tunon de Lara, M. Debled, Radiology 264, 597 (2012)

    Article  Google Scholar 

  • M.C. Peek, M. Ahmed, A. Napoli, B. ten Haken, S. McWilliams, S.I. Usiskin, S.E. Pinder, M. van Hemelrijck, M. Douek, Br. J. Surg. 102, 873 (2015)

    Article  Google Scholar 

  • A. Popovtzer, A. Mizrachi, M. Motiei, D. Bragilovski, L. Lubimov, M. Levi, O. Hilly, I. Ben-Aharon, R. Popovtzer, Nano 8, 2678 (2016)

    Google Scholar 

  • T. Qian, M. Chen, F. Gao, F. Meng, X. Gao, H. Yin, Magn. Reson. Imaging 32, 1052 (2014)

    Article  Google Scholar 

  • Q. Qin, Z.A. Xiong, Y. Liu, C.G. Yao, W. Zhou, Y.Y. Hua, Z.L. Wang, Mol. Med. Rep. 14, 2187 (2016)

    Google Scholar 

  • K.U. Schassburger, L. Lofgren, U. Lagerstedt, K. Leifland, K. Thorneman, B. Sandstedt, G. Auer, H. Wiksell, Breast 23, 152 (2014)

    Article  Google Scholar 

  • H.J. Scheffer, L.G. Vroomen, K. Nielsen, A.A. van Tilborg, E.F. Comans, C. van Kuijk, B.B. van der Meijs, J. van den Bergh, P.M. van den Tol, M.R. Meijerink, BMC Cancer 15, 772 (2015)

    Article  Google Scholar 

  • R.L. Siegel, K.D. Miller, A. Jemal, CA Cancer J. Clin. 65, 5 (2015)

    Article  Google Scholar 

  • N. Singla, J. Gahan, Curr. Opin. Urol. 26, 248 (2016)

    Article  Google Scholar 

  • E.S. Singletary, Breast Cancer 10, 4 (2003)

    Article  Google Scholar 

  • X. Song, M. Liang, W. Zhou, L. Ren, Z. Liu, G. Wang, Q. Wei, S. Wang, Int. J. Hyperth. 32, 265 (2016)

    Article  Google Scholar 

  • J.F. Waljee, E.S. Hu, L.A. Newman, A.K. Alderman, Cancer 112, 1679 (2008)

    Article  Google Scholar 

  • L. Wang, Q. Yao, J. Wang, G. Wei, G. Li, D. Li, R. Ling, J. Chen, Nucl. Med. Commun. 29, 137 (2008)

    Article  Google Scholar 

  • M. Wilson, S. Korourian, C. Boneti, L. Adkins, B. Badgwell, J. Lee, V. Suzanne Klimberg, Ann. Surg. Oncol. 19, 3192 (2012)

    Article  Google Scholar 

  • G. Zhong, J. Qi, S. Huo, H. Xue, Z. Xu, J. Li, Y. Zhou, M. Wu, L. Li, Onco Targets Ther 8, 3079 (2015)

    Article  Google Scholar 

  • M. Zhu, X.A. Lin, X.M. Zha, W.B. Zhou, T.S. Xia, S. Wang, PLoS One 10, e0120303 (2015)

    Article  Google Scholar 

Download references

Acknowledgments

Our special thanks will go to the Animal Experimental Center of South Medical University for helping us with rabbit experiments.

Author information

Authors and Affiliations

  1. Department of Hematology and Oncology, China-Japan Union Hospital of Jilin University, Changchun, 130033, China

    Wenlong Zhang

  2. Department of Gynecology and Obstetrics, The First Hospital of Jilin University, Changchun, 130021, China

    Wei Chai

  3. School of Medicine, Guangzhou Fuda Cancer Hospital, Jinan University, No.2, Tangde Xi Rd, Tianhe District, Guangzhou, 510665, China

    Jianying Zeng, Jibing Chen & Lizhi Niu

  4. Department of Rheumatology and Immunology, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130033, China

    Liqi Bi

Authors
  1. Wenlong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Chai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianying Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jibing Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Liqi Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lizhi Niu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Liqi Bi or Lizhi Niu.

Ethics declarations

Conflicts of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Source of funding

This work was supported by a grant from the International Science Foundation of Guangzhou Fuda Cancer Hospital (Y2015-ZD-001), China.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, W., Chai, W., Zeng, J. et al. Irreversible electroporation for the treatment of rabbit VX2 breast cancer. Biomed Microdevices 19, 29 (2017). https://doi.org/10.1007/s10544-017-0173-4

Download citation

  • Published:

  • DOI: https://doi.org/10.1007/s10544-017-0173-4

Keywords

Search

Navigation