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A concentration gradient generator on a paper-based microfluidic chip coupled with cell culture microarray for high-throughput drug screening

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Abstract

Inspired by the paper platforms for 3-D cell culture, a paper-based microfluidic device containing drug concentration gradient was designed and constructed for investigating cell response to drugs based on high throughput analysis. This drug gradient generator was applied to generate concentration gradients of doxorubicin (DOX) as the model drug. HeLa cells encapsulated in collagen hydrogel were incubated in the device reservoirs to evaluate the cell viability based on the controlled release of DOX spatially. It was demonstrated that drug diffusion through the paper fibers created a gradient of drug concentration, which influenced cell viability. This drug screening platform has a great opportunity to be applied for drug discovery and diagnostic studies with simultaneous and parallel tests of drugs under various gradient concentrations.

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Acknowledgments

This work was supported by a Tier 2 Academic Research Fund (ARC 22/13) and a Tier 1 Academic Research Fund (RG 37/14) from the Ministry of Education of Singapore awarded to Y.K. The Ph.D. scholarship from Nanyang Technological University awarded to P.X. is gratefully acknowledged. P.X. also thanks Yuli Kang for assistance in paper chip fabrication and setup.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Xiangyang Central Hospital, 136 Jingzhou Street, Xiangyang, Hubei, 441021, China

    Bo Hong

  2. School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore

    Peng Xue, Yafeng Wu, Jingnan Bao & Yon Jin Chuah

  3. Faculty of Materials and Energy, Institute for Clean Energy and Advanced Materials, Southwest University, 2 Tiansheng Road, Chongqing, Beibei, 400715, China

    Peng Xue & Yuejun Kang

Authors
  1. Bo Hong
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  2. Peng Xue
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  3. Yafeng Wu
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  4. Jingnan Bao
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  5. Yon Jin Chuah
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  6. Yuejun Kang
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Corresponding author

Correspondence to Yuejun Kang.

Additional information

B. Hong and P. Xue contributed equally to this work.

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Hong, B., Xue, P., Wu, Y. et al. A concentration gradient generator on a paper-based microfluidic chip coupled with cell culture microarray for high-throughput drug screening. Biomed Microdevices 18, 21 (2016). https://doi.org/10.1007/s10544-016-0054-2

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  • DOI: https://doi.org/10.1007/s10544-016-0054-2

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