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Microwell arrays for uniform-sized embryoid body-mediated endothelial cell differentiation

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Abstract

Embryonic stem (ES) cell is of great interest cell source in regenerating tissue constructs. We hypothesized that the interaction of cell-extracellular matrices (ECMs) would enable the control of ES cell differentiation pathway. We fabricated the hydrogel microwell array system to regulate uniform-sized embryoid bodies (EBs) and replate into various ECM components (e.g., gelatin, collagen I, fibronectin, laminin, and Matrigel). We demonstrated that collagen I and laminin largely induced ES cell-derived endothelial cell differentiation compared to gelatin. We also characterized ECMs-dependent endothelial cell differentiation by evaluating the endothelial gene expression, showing that Flk1 endothelial gene was highly expressed on collagen I. We also demonstrated the effect of the integrin on uniform-sized EBs-derived endothelial cell differentiation, showing that integrin α1 was largely expressed on laminin. Therefore, the cell-ECM interaction could be potentially powerful for controlling the uniform-sized EBs-derived endothelial cell differentiation.

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Acknowledgments

This paper was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (Grant Number 20110016331, 2012R1A1A2005822). This work was also supported by Sogang University Research Grant (201310012.01, SRF-201314004).

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Authors and Affiliations

  1. Department of Bionano Technology, Hanyang University, Ansan, Korea

    Ji-eun Kim

  2. Department of Mechanical Engineering, Sogang University, Seoul, Korea

    Jong Min Lee & Bong Geun Chung

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  1. Ji-eun Kim
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  2. Jong Min Lee
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  3. Bong Geun Chung
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Correspondence to Bong Geun Chung.

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Kim, Je., Lee, J.M. & Chung, B.G. Microwell arrays for uniform-sized embryoid body-mediated endothelial cell differentiation. Biomed Microdevices 16, 559–566 (2014). https://doi.org/10.1007/s10544-014-9858-0

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