Abstract
A microfluidic immunoassay system was developed for the study of the enhancement of protein binding reaction. The system mainly consisted of a thermopneumatic actuator and a reaction chamber. Reagent was pre-installed in the on-chip reservoir and manipulated by the actuator. Such design could eliminate the external tubing connections in order to reduce the waste of reagent and improve the portability. The on-chip actuator could manipulate the reagent bi-directionally to induce vortexes in the chamber. Enhancement of protein binding reaction was demonstrated by the protein model pair, i.e., mouse IgG and anti-mouse IgG. By such bi-directional fluid motion, more binding opportunities between suspended protein and its surface-immobilized counterpart were generated to improve the performance of immunoassay. It showed that an 83.74 % enhancement of the binding reaction was achieved, compared with the static situation. As a whole, the proposed microfluidic system is highly integrated and can enhance the protein binding efficiency using such novel design. The developed system can be easily extended to multi-reagents immunoassay protocols and provides a useful platform for point-of-care applications.
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Authors would like to thank Chang Gung University for the financial support (Project number: UERPD2B0121).
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Lei, K.F., Chen, KH. & Chang, YC. Protein binding reaction enhanced by bi-directional flow driven by on-chip thermopneumatic actuator. Biomed Microdevices 16, 325–332 (2014). https://doi.org/10.1007/s10544-014-9835-7
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DOI: https://doi.org/10.1007/s10544-014-9835-7