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On-chip immunomagnetic bead swarm based on magnetic actuation and mechanical vibration for biological detection

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Abstract

Immunomagnetic bead (IMB)-based detection has great potential for biomedical applications. Passive and active strategies, including microfluidics and magnetic actuation methods, have been developed to mix IMBs and analytes efficiently. However, cost-effective on-site detection using a simple microfluidic chip is challenging, and miniaturization of the magnetic driving device is imperative for portability. In this study, we propose a novel mixing method for an on-chip IMB swarm via magnetic actuation and mechanical vibration. A microfluidic chip system coupled with double spiral magnetic coils and a vibration motor was fabricated. The aggregation behavior of IMBs under magnetic fields and the diffusion behavior of the IMB swarm under mechanical vibration were analyzed in detail. Based on the synergetic effects of magnetic actuation and mechanical vibration, we achieved the highly efficient capturing of Vibrio parahaemolyticus DNA and goat anti-human immunoglobulin G by mixing the IMB swarm with the microfluidic chip. In this case, the antigen detection rate could reach ∼94.4%. Given its fascinating features, such IMB-microfluidic detection demonstrates significant potential for biomedical applications.

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Authors and Affiliations

  1. College of Engineering, China Agricultural University, Beijing, 100083, China

    JingWen Pan, Rehan Saeed, KaiHeng Cao, KeHan Chen & WenQiang Zhang

  2. School of Mechanical Engineering & Automation, Beihang University, Beijing, 100191, China

    De Gong, Jun Cai & DeYuan Zhang

  3. Department of Nutrition and Health, China Agricultural University, Beijing, 100193, China

    Yuan Su & WenTao Xu

Authors
  1. JingWen Pan
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  2. De Gong
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  3. Rehan Saeed
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  4. KaiHeng Cao
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  5. KeHan Chen
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  6. Yuan Su
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  7. WenQiang Zhang
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  8. WenTao Xu
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  9. Jun Cai
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  10. DeYuan Zhang
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Corresponding author

Correspondence to WenQiang Zhang.

Additional information

This work was supported by the National Natural Science Foundation of China (Grant No. 51975574) and the Fundamental Research Funds for the Central Universities (Grant No. 2020TC017).

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Pan, J., Gong, D., Saeed, R. et al. On-chip immunomagnetic bead swarm based on magnetic actuation and mechanical vibration for biological detection. Sci. China Technol. Sci. 65, 2573–2581 (2022). https://doi.org/10.1007/s11431-022-2169-6

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  • DOI: https://doi.org/10.1007/s11431-022-2169-6

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