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Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells

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Abstract

Deficiency of female hormones is the principal cause of menopausal syndrome. The aim of this study was to establish a co-microencapsulation model of ovarian granulosa cells (GCs) and theca cells (TCs), and to investigate its endogenous release of female hormones. Rat ovarian GCs and TCs were isolated and co-microencapsulated in alginate-chitosan-alginate microcapsules. The effects of cell number ratio of GCs/TCs on syntheses of estradiol (E2) and progesterone (P4) were investigated in vitro. Serum levels of E2 and P4 in ovariectomized rats were measured for 60 days after the co-microencapsulated GCs and TCs were transplanted. It was showed that E2 synthesis in vitro was influenced by cell number ratio of GCs/TCs and the ratio for the maximum synthesis was 1:2. Serum E2 and P4 levels could be maintained normal for 60 days by the co-microencapsulated GCs/TCs. Transplantation of co-microencapsulated GCs/TCs may be a promising approach to provide endogenous female hormones for menopausal syndrome.

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Acknowledgments

This work was supported by Chinese Natural Science Foundation (No. 30973682) and Key Project of Chinese National Programs for Fundamental Research and Development (973 Program) (No. 2009CB930300).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Pharmaceutics, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Cong Liu, Xiaolu Luan, Ying He, Xiaoping Xia, Liangliang Sun, Wang Miao, Yi Jin & Longxiao Liu

  2. Women’s Hospital, Zhejiang University, Hangzhou, 310006, People’s Republic of China

    Xiaoping Xia

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  1. Cong Liu
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  2. Xiaolu Luan
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  3. Ying He
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  4. Xiaoping Xia
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  5. Liangliang Sun
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  7. Yi Jin
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  8. Longxiao Liu
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Correspondence to Longxiao Liu.

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Liu, C., Luan, X., He, Y. et al. Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells. Biomed Microdevices 16, 209–216 (2014). https://doi.org/10.1007/s10544-013-9824-2

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  • DOI: https://doi.org/10.1007/s10544-013-9824-2

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