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Development of a microplate reader compatible microfluidic chip for ELISA

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Abstract

We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related procedures including the introduction of reagents, dilution and distribution of samples, as well as immobilization of enzyme can be readily carried out on a poly (dimethylsiloxane) (PDMS) chip. Particularly, this microfluidic chip comprising of two distinct parallel units, and has an identical dimension as a conventional microtiter plate, which offers access to the directly quantitative detection by the microplate reader. Gradient-concentration reacting solutions at six different concentrations level generated by the microfluidic channel network are simultaneously transported to 24 reaction chambers to form enzymatic products. Alkaline phosphatase (ALP), 4-methylumbelliferyl phosphate (4-MUP) and KH2PO4 are used as enzyme-substrate-inhibitor model, to demonstrate the utility of the developed microchip-based enzyme inhibitor assay. Various conditions such as the surface treatment of chip channels, fluids velocities, substrate concentration, and buffer pH are investigated. The present microfluidic device for ELISA holds several advantages, for instance frugal usage of samples and reagents, less of operating time, favorably integrated configuration, ease of manipulation, and could be explored to a variety of high throughput drug screening.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20875105).

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Authors and Affiliations

  1. School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou, 510006, China

    Fenghua Hou, Qin Zhang, Jianping Yang, Xinchun Li, Xiujuan Yang, Shuping Wang & Zhiyi Cheng

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  1. Fenghua Hou
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  2. Qin Zhang
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  3. Jianping Yang
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  4. Xinchun Li
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  5. Xiujuan Yang
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  6. Shuping Wang
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  7. Zhiyi Cheng
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Correspondence to Zhiyi Cheng.

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Hou, F., Zhang, Q., Yang, J. et al. Development of a microplate reader compatible microfluidic chip for ELISA. Biomed Microdevices 14, 729–737 (2012). https://doi.org/10.1007/s10544-012-9654-7

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