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Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

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Abstract

In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK 293 cells on those patterns during culture over several days is examined. The first patterning approach described is based on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (–NH2) > ox-PDMS (–OH) >> PSS (–SO3 −) > PDMS (–CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering the interplay between individual cell–surface interactions and a permanent cell tractional force.

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Acknowledgements

Financial support from European Union 6th Framework project “CellPROM” (NMP4-CT-2004-500039) is gratefully acknowledged. Dr. H. Pick (LCPPM, EPFL, Switzerland) is thanked for the kind provision of human embryonic kidney cells.

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Authors and Affiliations

  1. Fraunhofer-Institute for Biomedical Engineering, IBMT, D-66386, St. Ingbert, Germany

    R. M. Johann

  2. Silicon Biosystems, I-40138, Bologna, Italy

    Ch. Baiotto

  3. Swiss Federal Institute of Technology, EPFL, STI-LMIS, CH-1015, Lausanne, Switzerland

    Ph. Renaud

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  1. R. M. Johann
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  2. Ch. Baiotto
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  3. Ph. Renaud
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Correspondence to R. M. Johann.

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Johann, R.M., Baiotto, C. & Renaud, P. Micropatterned surfaces of PDMS as growth templates for HEK 293 cells. Biomed Microdevices 9, 475–485 (2007). https://doi.org/10.1007/s10544-007-9054-6

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  • DOI: https://doi.org/10.1007/s10544-007-9054-6

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