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Reconstitution of Okenone into Light Harvesting Complexes from Allochromatium minutissimum

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Abstract

Okenone was reconstituted into light harvesting (LH) complexes of the purple photosynthetic bacterium Allochromatium minutissimum possessing the spirilloxanthin pathway for carotenoid biosynthesis. Suppression of this pathway by diphenylamine, an inhibitor of carotenogenesis, yielded nearly carotenoidless complexes preserving their native spectral properties. Using a previously developed technique, okenone was readily reconstituted into LH1 complex (>90%) whereas its reconstitution into LH2 complex was of low efficacy (10–20%). The absorption band of the reconstituted okenone was shifted to shorter wavelength compared with its position in vivo. This is typical for other reconstituted carotenoids. The reconstitution of okenone was confirmed by Li-DS electrophoresis (in contrast to free okenone the reconstituted okenone migrated with complexes), circular dichroism spectra (reconstituted okenone exhibited optical activity), and fluorescence excitation spectrum (energy transfer from okenone to bacteriochlorophyll was at the control level).

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Abbreviations

Alc. minutissimum:

Allochromatium minutissimum

Mac. purpuratum:

Marichromatium purpuratum

Rba. sphaeroides:

Rhodobacter sphaeroides

Rsp. rubrum:

Rhodospirillum rubrum

Li-DS:

lithium dodecyl sulfate

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Authors and Affiliations

  1. Institute of Basic Problems of Biology, Russian Academy of Sciences, ul. Institutskaya 2, 142290, Pushchino, Moscow Region, Russia

    O. A. Toropygina, Z. K. Makhneva & A. A. Moskalenko

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  1. O. A. Toropygina
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  2. Z. K. Makhneva
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  3. A. A. Moskalenko
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Correspondence to A. A. Moskalenko.

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__________

Translated from Biokhimiya, Vol. 70, No. 11, 2005, pp. 1498–1505.

Original Russian Text Copyright © 2005 by Toropygina, Makhneva, Moskalenko.

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Toropygina, O.A., Makhneva, Z.K. & Moskalenko, A.A. Reconstitution of Okenone into Light Harvesting Complexes from Allochromatium minutissimum . Biochemistry (Moscow) 70, 1231–1237 (2005). https://doi.org/10.1007/s10541-005-0252-7

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  • DOI: https://doi.org/10.1007/s10541-005-0252-7

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