Abstract
The effect of a moderate (“soft”) uncoupling of mitochondria on the lifespan and some parameters of biological age of Drosophila melanogaster strain Oregon was studied. Addition of the uncoupler 2,4-dinitrophenol (DNP) to the nutritional mixture of larvae significantly increased the average lifespan of the flies without changing their maximal lifespan. DNP significantly increased the rate of oxygen consumption by isolated mitochondria and tissue homogenates of the flies in state 4 (of Chance). DNP also decreased the activity of alcohol dehydrogenase (a parameter of flies' biological age)) in the tissue homogenates, especially on octanol as the reaction substrate. However, being deprived of food the DNP-treated flies displayed a markedly decreased viability as compared to the control flies. On the whole, the results suggest that “soft” uncoupling of mitochondria may increase the lifespan.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ROS:
-
reactive oxygen species
- DNP:
-
2,4-dinitrophenol
- Hsp70:
-
70-kD heat shock protein
- EGTA:
-
ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid
REFERENCES
Anisimov, V. N. (2003) Molecular and Physiological Mechanisms of Aging [in Russian], Nauka, St. Petersburg, pp. 5–6.
Skulachev, V. P. (1998) Biochemistry (Moscow), 63, 1335–1343.
Korshunov, S. S., Skulachev, V. P., and Starkov, A. A. (1997) FEBS Lett., 416, 15–18.
Skulachev, V. P. (2004) Aging Cell, 3, 17–19.
Brand, M. D. (2000) Exp. Gerontol., 35, 811–820.
Medvedev, N. N. (1966) Practical Genetics [in Russian], Nauka, Moscow, pp. 26–45.
Roberts, D. B. (1986) in Drosophila. A Practical Approach (Roberts, D. B., ed.) Oxford, Washington, pp. 1–38.
Echtay, K. S., Esteves, T. C., Pakay, J. L., Jekabsons, M. B., Lambert, A. J., Portero-Otin, M., Pamplona, R., Vidal-Puig, A. J., Wang, S., Roebuck, S. J., and Brand, M. D. (2003) The EMBO J., 22, 4103–4110.
Zelensky, M. I. (1986) Polarographic Determination of Oxygen in Studies on Photosynthesis and Respiration [in Russian], Nauka, Leningrad, pp. 77–109.
Bulos, B. A., Thomas, B. J., and Shukla, S. J. (1984) Arch. Biochem. Biophys., 234, 382–393.
Sofer, W., and Ursprung, H. (1968) J. Biol. Chem., 243, 3110–3115.
Miller, G. I. (1959) Analyt. Chem., 31, 964–966.
Miquel, J., Fleming, J., and Economos, A. C. (1982) Arch. Gerontol. Geriatr., 1, 159–165.
Barros, M. H., Bandy, B., Tahara, E. B., and Kowaltowski, A. J. (2004) J. Biol. Chem., 279, 49883–49888.
Belokon', E. M., and Chernik, Ya. I. (1986) Ontogenez, 17, 278–284.
Filippovich, Yu. B., and Konichev, A. S. (1987) Multiple Forms of Insects' Enzymes and Problems of Agricultural Entomology [in Russian], Nauka, Moscow, pp. 35–98.
Heintra, P. W., Scharloo, W., and Thorig, G. E. (1986) Comp. Biochem. Physiol., B83, 409–419.
Soliman, M. H. (1987) Gerontology (Schwaiz), 33, 57–63.
Krebs, R. A., and Holbrook, S. H. (2001) Biochem. Genet., 39, 73–82.
Ellgaard, E. G., and Maxwell, B. L. (1975) Cell Differ., 3, 379–387.
Rodrigo, G. C., Lawrence, C. L., and Standen, N. B. (2001) J. Physiol., 533P.
Finkel, T., and Holbrook, N. J. (2000) Nature, 408, 239–247.
Skulachev, V. P. (2003) Top. Curr. Genet., 3, 191–238.
Author information
Authors and Affiliations
Additional information
__________
Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1193–1197.
Original Russian Text Copyright © 2005 by Padalko.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-238, March 27, 2005.
Rights and permissions
About this article
Cite this article
Padalko, V.I. Uncoupler of Oxidative Phosphorylation Prolongs the Lifespan of Drosophila . Biochemistry (Moscow) 70, 986–989 (2005). https://doi.org/10.1007/s10541-005-0213-1
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s10541-005-0213-1