Abstract
The effect of cultivation time and concentration of inorganic phosphate (Pi) in the culture medium on the accumulation of polyphosphates (polyP) and the activity of two cytosolic exopolyphosphatases of the yeast Saccharomyces cerevisiae was studied: an exopolyphosphatase of 40 kD encoded by PPX1 and a high molecular weight exopolyphosphatase encoded by another gene. Depletion of polyP in the cells on Pi starvation is a signal factor for the accumulation of polyP after the subsequent addition of 5–20 mM Pi and glucose to the cells or spheroplasts. A high activity of both exopolyphosphatases does not prevent the accumulation of polyP. The expression of the high molecular weight exopolyphosphatase is due to the acceleration of metabolism in cells that have reached the stage of growth deceleration on the addition of Pi and glucose or complete culture medium. This process may occur independently from the accumulation of polyP. The activity of exopolyphosphatase PPX1 depends less on the mentioned factors, decreasing 10-fold only under conditions of phosphate surplus at the stationary growth stage.
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REFERENCES
Kulaev, I. S. (1975) Biochemistry of High Molecular Weight Polyphosphates [in Russian], MGU Publishers, Moscow.
Kulaev, I. S., Vagabov, V. M., and Kulakovskaya, T. V. (2004) The Biochemistry of Inorganic Polyphosphates, Wiley.
Kornberg, A., Rao, N. N., and Ault-Riche, D. (1999) Ann. Rev. Biochem., 68, 89–125.
Vagabov, V. M., Trilisenko, L. V., and Kulaev, I. S. (2000) Biochemistry (Moscow), 65, 349–354.
Lichko, L. P., Andreeva, N. A., Kulakovskaya, T. V., and Kulaev, I. S. (2003) FEMS Yeast Res., 3, 233–238.
Andreeva, N. A., Kulakovskaya, T. V., and Kulaev, I. S. (2001) Biochemistry (Moscow), 66, 147–153.
Trilisenko, L. V., Andreeva, N. A., Kulakovskaya, T. V., Vagabov, V. M., and Kulaev, I. S. (2003) Biochemistry (Moscow), 68, 577–581.
Andreeva, N. A., Kulakovskaya, T. V., and Kulaev, I. S. (2004) Biochemistry (Moscow), 69, 387–393.
Wurst, H., Shiba, T., and Kornberg, A. (1995) J. Bacteriol., 177, 898–906.
Andreeva, N. A., and Okorokov, L. A. (1993) Yeast, 9, 127–139.
Persson, B. L., Lagerstedt, J. O., Pratt, J. R., Pattison-Granberg, J., Lundh, K., Shokrollahzadeh, S., and Lundh, F. (2003) Curr. Genet., 43, 225–244.
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Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1186–1192.
Original Russian Text Copyright © 2005 by Kulakovskaya, Andreeva, Trilisenko, Suetin, Vagabov, Kulaev.
Originally published in Biochemistry (Moscow) On Line Papers in Press, as Manuscript BM04-212, December 5, 2004.
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Kulakovskaya, T.V., Andreeva, N.A., Trilisenko, L.V. et al. Accumulation of Polyphosphates and Expression of High Molecular Weight Exopolyphosphatase in the Yeast Saccharomyces cerevisiae . Biochemistry (Moscow) 70, 980–985 (2005). https://doi.org/10.1007/s10541-005-0212-2
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DOI: https://doi.org/10.1007/s10541-005-0212-2