Skip to main content
SpringerLink
Log in

Anticoagulant Modulation of Blood Cells and Platelet Reactivity by Garlic Oil in Experimental Diabetes Mellitus

  • Published:
Bioscience Reports

Abstract

Multiple blood cell types and metabolic pathways involved in the modulation of platelet reactivity were investigated in streptozotocin-induced diabetic rats treated with garlic oil. Platelet counts of diabetic rats treated with garlic oil were significantly (P<0.01) reduced as compared to diabetic control rats. Garlic oil also increased the leucocyte counts of diabetic rats as compared to diabetic control rats. The significant (P<0.001) decreases by garlic oil of plasma concentration factors, V, VII, VIII: C, IX and X in diabetic rats may be interpreted to mean that there was a modulation of factor VII similar to that brought about by thrombin on factors V and VIII: C. This reversal of hypercoagulation through integrated biochemical reaction is suggestive of multicellular modulation of platelet reactivity, erythrocytes and neutrophils and the functional interactions between plasma coagulation factors and platelet cofactors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adoga GI, Ohaeri OC (1991) Effects of garlic oil on prothrombin, thrombin and partial thromboplastin times in streptozotocin-induced diabetic rats. Med Sci Res 19:407–408

    CAS  Google Scholar 

  • Broze GJ Jr (1995) Tissue factor pathway inhibitor and the current concept of coagulation. Blood Coagul Fibrinolysis 6:S7–S12

    Article  PubMed  CAS  Google Scholar 

  • Brozovic M, Mackie I (1991) Investigation of acute haemostatic failure. In: Dacie JV, Lewis SM (eds) Practical haematology 7th edn. Churchill Livingstone, London, pp 281–282

    Google Scholar 

  • Ceriello A, Giugliano D, Quatraro A, Dello Russo P, Torella R (1988) Blood glucose may condition FVII levels in diabetic and normal subjects. Diabetilogia 31:889–891

    CAS  Google Scholar 

  • Ceriello A,Giacomello R, Colatutto A, Taboga C, Gonano F (1995) Hyperglycaemia induced thrombin formation in diabetes: the possible role of oxidative stress. Diabetes 44:924–928

    Article  PubMed  CAS  Google Scholar 

  • Dacie JV and Lewis SM (1991) Basic haematological techniques. Diabetes 44:924–928

    Google Scholar 

  • Gerstein HC, Yusuf S (1996) Dysglycaemia and risk of cardiovascular disease. Lancet 347:949–950

    Article  PubMed  CAS  Google Scholar 

  • Kannel WB, D’Agostino RB, Wilson PW, Belanger AJ, Gagnon DR (1990) Diabetes, Fibrinogen, and risk of cardiovascular disease: the Framingham experince. Am Heart J 120:672–676

    Article  PubMed  CAS  Google Scholar 

  • Kwaan HC (1992) Changes in blood coagulation, platelet function, and plasminogen-plasmin system in diabetes. Diabetes 41(Suppl. 2):32–35

    PubMed  Google Scholar 

  • Laasko M, Lehto S (1997) Epidemiology of macrovascular disease in diabetes. Diabetes Rev 5:294–315

    Google Scholar 

  • Makheja AN, Bailey JM (1990) Antiplatelet constituent of garlic and onion. Agents Act 29:360–363

    Article  CAS  Google Scholar 

  • Marcus AJ (1990) Thrombosis and inflammation as multicellular processes. Pathophysiolological significance of transcellular metabolism. Blood 76:1903–1907

    PubMed  CAS  Google Scholar 

  • Marcus AJ, Safier LB (1993) Thromboregulation: multicellular modulation of platelet reactivity in haemostasis and thrombosis. Fed Am Soc Exp Biol J 7(6):516–522

    CAS  Google Scholar 

  • Miller GJ, Stirling Y, Esnouf MP, Heinrich J, van de Loo J, Kienast J, Wu KK, Morrissey JH, Meade TW, Martin JC (1994) FVII-deficient substrate plasmas depleted of protein C raise the sensitivity of the FVII bio-assay to activated FVII: an international study. Thromb Haemost 71:38–48

    PubMed  CAS  Google Scholar 

  • Morrissey JH, Macik BG, Neuenschwander PF, Comp PC (1993) Quantitation of activated FVII levels in plasma using a tissue factor mutant selectivity deficient in promoting FVII activation. Blood 81:743–744

    Google Scholar 

  • Muntean WE, Borkenstein MH, Haas J (1985) Elevation of FVIII over FVIII coagulant antigen in diabetic children without vascular disease: a sign of activation of the FVIII coagulant moiety during poor diabetes control. Diabetes 34:140–144

    Article  PubMed  CAS  Google Scholar 

  • Nemerson Y (1992) Tissue factor pathway of blood coagulation. Semin Haematol 2:170–176

    Google Scholar 

  • Ohaeri OC (1999) Renal function and calcium metabolism in streptozotocin-diabetic rats treated with garlic oil. Med Sci Res 27:443–444

    Google Scholar 

  • Ohaeri OC (2001) Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Biosci Rep 21(1):19–24

    Article  PubMed  CAS  Google Scholar 

  • Osterman H, van de Loo J (1986) Factors of the hemostatic system in diabetic patients. Haemostasis 16:386–416

    Google Scholar 

  • Rapaport SI, Rao LVM (1995) The tissue factor pathway: how it has become a “prima ballerina”. Thromb Haemost 74:7–17

    PubMed  CAS  Google Scholar 

Download references

Acknowledgment

The author acknowledges the financial contribution of the Federal Ministry of Health and Social Welfare Nigeria to O.C. O haeri. The data in this paper formed part of a PhD thesis submitted to the University of Jos, Nigeria. We are grateful to Professor Z.S.C. Okoye for useful comments and advice.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medical Sciences, University of JOS, JOS, Nigeria

    O.C. Ohaeri & G.I. Adoga

  2. Department of Chemical Sciences, Michael Okpara University of Agriculture, Umudike, PMB 7267, Umuahia, Abia state, Nigeria

    O.C. Ohaeri

Authors
  1. O.C. Ohaeri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G.I. Adoga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O.C. Ohaeri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohaeri, O., Adoga, G. Anticoagulant Modulation of Blood Cells and Platelet Reactivity by Garlic Oil in Experimental Diabetes Mellitus. Biosci Rep 26, 1–6 (2006). https://doi.org/10.1007/s10540-006-9004-1

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10540-006-9004-1

Keywords

Search

Navigation