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Cloning and characterization of four TpSnRK2s from dwarf Polish wheat

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Biologia Plantarum

Abstract

Protein phosphorylation/dephosphorylation is a major signalling event induced by abiotic stresses in plants. Sucrose nonfermenting 1-related protein kinase 2 (SnRK2) plays important roles in response to osmotic stress. In the present study, four SnRK2s, TpSnRK2.1/3/7/8, were cloned and characterized from Triticum polonicum L. (dwarf Polish wheat, DPW, AABB). All of these were individually located on 2AL, 1AL, 2AL, and 5BL. Two spliced isoforms of TpSnRK2.8 (TpSnRK2.8a and TpSnRK2.8b) were observed. TpSnRK2.1 and TpSnRK2.3 were classified into the group II; TpSnRK2.7 was classified into the group I; and TpSnRK2.8a/b were classified into the group III. Expression patterns revealed that TpSnRK2.1 responded to cold, NaCl, polyethylene glycol (PEG), and abscisic acid (ABA) in both roots and leaves; TpSnRK2.3 was strongly regulated by cold, NaCl, and ABA in both roots and leaves, and by PEG in roots; TpSnRK2.7 was induced by NaCl and PEG in roots, but was not activated by ABA; and TpSnRK2.8s were significantly activated by cold, NaCl, PEG, and ABA in both roots and leaves. From the above results, we inferred that TpSnRK2.1/3/8 may participate in the responses to environmental stresses in ABA-dependent signal transduction pathway but TpSnRK2.7 is possibly involved in responses to environmental stresses in a non-ABA-dependent manner. They play important roles in specific tissues under different stresses.

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Abbreviations

AA:

amino acid

ABA:

abscisic acid

AMPKs:

AMP-activated protein kinases

DPW:

dwarf Polish wheat

ORF:

open reading frame

PEG:

polyethylene glycol

qPCR:

quantitative PCR

SnRK:

sucrose nonfermenting 1-related protein kinase

UTR:

untranslated region

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Authors and Affiliations

  1. College of Landscape Architecture, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, P.R. China

    Y. Jiang, Z. Huang & X. Yu

  2. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, P.R. China

    Y. Wang, H. Kang, L. Sha, X. Fan, H. Zhang & Y. Zhou

  3. Landscape Architecture Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, P.R. China

    S. Gao

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  1. Y. Jiang
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  2. Y. Wang
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  3. Z. Huang
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  4. H. Kang
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  10. X. Yu
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Correspondence to X. Yu.

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Acknowledgments: The authors thank the National Natural Science Foundation of China (Nos. 31671688 and 31470305), the Bureau of Science and Technology, and the Bureau of Education of Sichuan Province, China.

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Jiang, Y., Wang, Y., Huang, Z. et al. Cloning and characterization of four TpSnRK2s from dwarf Polish wheat. Biol Plant 61, 601–610 (2017). https://doi.org/10.1007/s10535-017-0719-6

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  • DOI: https://doi.org/10.1007/s10535-017-0719-6

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