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Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

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Biologia Plantarum

Abstract

The accumulation of polysaccharides, activities of sucrose metabolism enzymes, and the expression of sucrose biosynthetic genes in Dendrobium officinale were significantly affected by exogenous methyl jasmonate (MeJA). Application of MeJA increased the content of polysaccharides and the highest polysaccharide production occurred in the samples treated with 200 μM MeJa. The MeJA application influenced polysaccharide biosynthesis rather than degradation because the activities of sucrose metabolism enzymes and the expressions of sucrose biosynthetic genes were upregulated by MeJA. Interestingly, low MeJA concentrations promoted accumulation of Dendrobium polysaccharides, while high MeJA amounts played an inhibitory role. The content of major constituent of polysaccharides, glucose and mannose, also increased after MeJa treatment.

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Abbreviations

EDTA:

ethylene diamine tetraacetic acid

HPLC:

high performance liquid chromatography

MeJA:

methyl jasmonate

MOPS:

3-(morpholino) propanesulfonic acid

MS:

Murashige and Skoog

PDAD:

photodiode array detector

PMP:

3-methyl-1-phenyl-2-pyrazolin-5-one

SPS:

sucrose phosphate synthase

SS:

sucrose synthase

UDPG:

uridine diphosphate glucose

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Authors and Affiliations

  1. School of Life Sciences, Shandong University of Technology, Zibo, Shandong, 255049, P.R. China

    Z. Q. Yuan, J. Y. Zhang & T. Liu

Authors
  1. Z. Q. Yuan
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  2. J. Y. Zhang
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  3. T. Liu
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Correspondence to J. Y. Zhang.

Additional information

Acknowledgements: The research was supported by the National Natural Science Foundation of China (Nos. 31100377 and 41201518) and the Project of Shandong Province Higher Educational Science and Technology Program (No. J16LE02).

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Yuan, Z.Q., Zhang, J.Y. & Liu, T. Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate. Biol Plant 61, 438–444 (2017). https://doi.org/10.1007/s10535-016-0702-7

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  • DOI: https://doi.org/10.1007/s10535-016-0702-7

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