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Soybean NAC gene family: sequence analysis and expression under low nitrogen supply

  • Original paper
  • Published:
Biologia Plantarum

Abstract

NAM, ATAF1/2, and CUC2 (NAC) proteins are plant-specific transcription factors playing essential roles in plant development and various abiotic stress responses. In the present study, we identified 173 full-length NAC genes in soybean, which were phylogenetically clustered into 15 groups (NACa - NACo). The soybean NAC genes (GmNACs) were non-randomly located across the 20 chromosomes, and 128 genes (86.5 %) were preferentially located in duplicated regions of chromosome arms, which implied long segmental duplication and contributed to evolution of the GmNAC gene family. Most GmNACs genes showed a distinct tissue-specific expression pattern and the redundant expression patterns of active duplicate genes suggested that GmNACs have been retained by substantial subfunctionalization during soybean evolution. Furthermore, active GmNACs genes that had undergone strong artificial selection during soybean domestication were identified based on selection analysis. After low nitrogen treatment, enhanced expression of some selected GmNAC genes were noticed in soybean shoot and root, which implied that GmNACs might play an important role in nitrogen metabolism. Here, we summarize the sequence and expression analysis of the NAC gene family in the soybean.

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Abbreviations

AREB:

ABA responsive element binding protein

ATAF:

Arabidopsis transcription activation factor

CUC:

cup-shaped cotyledon

Mya:

milliard year ago

NAC:

NAM, ATAF1/2 and CUC2

NAM:

no apical meristem

SNP:

single nucleotide polymorphism

TF:

transcription factor

WRKY:

WRKY DNA-binding protein

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Authors and Affiliations

  1. Department of Agronomy Sciences, Anhui Agricultural University, Hefei, 230036, P.R. China

    X. Wang, D. Li, J. Jiang, Z. Dong & Y. Ma

  2. Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei, 230036, P.R. China

    X. Wang, D. Li & J. Jiang

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Correspondence to X. Wang.

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Acknowledgments: This study was supported by grants from the Natural Science for Key Project of Anhui Education Committee (No. KJ2016A843), the Cultivating Academic Backbone Fundation of Anhui Agricultural University (No. 2014XKPY-04), the Introduced Leading Talent Research Team for Universities in Anhui Province and the Special Fund for Agro-scientific Research in the Public Interest (No. 201503121-02). We wish to thank the anonymous reviewers for their helpful comments on this manuscript.

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Wang, X., Li, D., Jiang, J. et al. Soybean NAC gene family: sequence analysis and expression under low nitrogen supply. Biol Plant 61, 473–482 (2017). https://doi.org/10.1007/s10535-016-0693-4

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