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Effect of exogenous abscisic acid on cold acclimation in two Magnolia species

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Biologia Plantarum

Abstract

In northern China, freezing injury is observed frequently in the rare species Magnolia wufengensis but not in the more common species Magnolia denudata. To investigate the role of the phytohormone abscisic acid (ABA) on frost tolerance in these two species, exogenous ABA was applied to the seedlings and then physiological and biochemical responses were measured during cold acclimation. Shoot growth cessation was stimulated by ABA in M. wufengensis but not in M. denudata. Abscisic acid inhibited shoot growth in M. wufengensis but not in M. denudata. Treatment with ABA stimulated leaf senescence in both species, and this effect was greater in M. denudata. For both species, ABA-treated plants exhibited bud dormancy sooner and had an increased tolerance to freezing, decreased water content and increased accumulation of proline, glucose, and fructose in shoots. These effects were generally greater for M. denudata. Freezing tolerance was significantly correlated with content of water, proline, glucose, and fructose for both species, but freezing tolerance was significantly correlated with raffinose content only in M. wufengensis. We conclude that exogenous ABA could increase cold acclimation and improve cold hardiness of both Magnolia species, although M. denudata was more responsive to ABA than M. wufengensis, which might result from a greater dehydration and accumulation of proline and certain soluble sugars.

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Abbreviations

ABA:

abscisic acid

D50B:

number of days to 50 % budburst

LT50 :

low temperature representing 50 % relative electrolyte leakage

PN :

net photosynthetic rate

References

  • Alvim, R., Hewett, E., Saunders, P.F.: Seasonal variation in the hormone content of willow. — Plant Physiol. 57: 474–476, 1976.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Anchordoguy, T., Rudolph, A., Carpenter, J., Crowe, J.: Modes of interaction of cryoprotectants with membrane phospholipids during freezing. — Cryobiology 24: 324–331, 1987.

    Article  CAS  PubMed  Google Scholar 

  • Bates, L.S., Waldren, R.P., Teare, L.D.: Rapid determination of free proline for water stress studies. — Plant Soil 39: 205–207, 1973.

    Article  CAS  Google Scholar 

  • Bravo, A., Zuniga, G.E., Alberdi, M., Corcuera, L.J.: The role of ABA in freezing tolerance and cold acclimation. — Physiol Plant. 103: 17–23, 1998.

    Article  CAS  Google Scholar 

  • Churchill, G.C., Reaney, M.J.T., Abrams, S.R., Gusta, L.V.: Effects of abscisic acid and abscisic acid analogs on the induction of freezing tolerance of winter rye (Secale cereale L.) seedlings. — Plant Growth Regulat. 25: 35–45, 1998.

    Article  CAS  Google Scholar 

  • Cox, S.E., Stushnoff, C.: Temperature-related shifts in soluble carbohydrate content during dormancy and cold acclimation in Populus tremuloides. — Can. J. Forest Res. 31: 730–737, 2001.

    Article  CAS  Google Scholar 

  • Dallaire, S., Houde, M., Gagne, Y., Saini, H.S., Boileau, S.: ABA and low-temperature induce freezing tolerance via distinct regulatory pathways in wheat. — Plant Cell Physiol. 35: 1–9, 1994.

    CAS  Google Scholar 

  • Dhont, C.: Nitrogen reserves, spring regrowth and winter survival of field-grown alfalfa (Medicago sativa) defoliated in the autumn. — Ann. Bot. 97: 109–120, 2006.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gusta, L.V., Wisniewski, M., Nesbitt, N.T., Gusta, M.L.: The effect of water, sugars and proteins on the pattern of ice nucleation and propagation in acclimated an nonacclimated canola leaves. — Plant Physiol. 135: 1642–1653, 2004.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guy, C.L.: Freezing tolerance of plants: current understanding and selected emerging concepts. — Can. J. Bot. 81: 1216–1223, 2003.

    Article  CAS  Google Scholar 

  • Guy, C.L., Huber, J.L.A., Huber, S.C.: Sucrose phosphate synthase and sucrose accumulation at low temperature. — Plant Physiol. 100: 502–508, 1992.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hare, P.D., Cress, W.A.: Metabolic implications of stress-induced proline accumulation in plants. — J. Plant Growth Regul. 21: 79–102, 1997.

    Article  CAS  Google Scholar 

  • Hincha, D., Bakaltcheva, I., Schmitt, J.: Galactose specific lectins protect isolated thylakoids against freeze-thaw damage. — Plant Physiol. 103: 59–65, 1993.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jones, K., Paroschy, J., McKersie, B., Bowley, S.: Carbohydrate composition and freezing tolerance of canes and buds in Vitis vinifera. — J Plant Physiol. 155: 101–106, 1999.

    Article  CAS  Google Scholar 

  • Jun, H.L., Duk, J.Y., Su, J.K., Doil, C., Hee, J.L.: Intraspecies differences in cold hardiness, carbohydrate content and β-amylase gene expression of Vaccinium corymbosum during cold acclimation and deacclimation. — Tree Physiol. 32: 1533–1540, 2012.

    Article  Google Scholar 

  • Kasuga, J., Arakawa, K., Fujikawa, S.: High accumulation of soluble sugars in deep supercooling Japanese white birch xylem parenchyma cells. — New Phytol. 174: 569–579, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Keller, M.: The Science of Grapevines: Anatomy and Physiology. 1th Ed. - Academic Press, Burlington 2010.

    Google Scholar 

  • Koussa, T., Cherrad, M., Bertrand, A., Broquedis, M.: Comparison of the contents of starch, soluble carbohydrates and abscisic acid of latent buds and internodes during the vegetative cycle of grapevine. — Vitis 37: 5–10, 1998.

    CAS  Google Scholar 

  • Kozlowski, T.T., Pallardy, S.G.: Acclimation and adaptive responses of woody plants to environmental stresses. — Bot Rev. 68: 270–334, 2002.

    Article  Google Scholar 

  • Kucera, B., Cohn, A., Leubner-Metzger, G.: Plant hormone interactions during seed dormancy release and germination. — Seed Sci. Res. 15: 281–307, 2005.

    Article  CAS  Google Scholar 

  • Li, C., Junttila, O., Heino, P., Palva, E.T.: Different responses of northern and southern ecotypes of Betula pendula to exogenous ABA application. — Tree Physiol. 23: 481–487, 2003.

    Article  CAS  PubMed  Google Scholar 

  • Li, C., Wu, N., Liu, S.: Development of freezing tolerance in different altitudinal ecotypes of Salix paraplesia. — Biol. Plant. 49: 65–71, 2005a.

    Article  Google Scholar 

  • Li, C., Yang, Y., Junttila, O., Palva, E.T.: Sexual differences in cold acclimation and freezing tolerance development in sea buckthorn (Hippophae rhamnoides L.) ecotypes. — Plant Sci. 168: 1365–1370, 2005b.

    Article  CAS  Google Scholar 

  • Lim, C.C., Arora, R.: Comparing Gompertz and Richards functions to estimate freezing injury in Rhododendron using electrolyte leakage. — J. amer. Soc. hort. Sci. 123: 246–252, 1998.

    Google Scholar 

  • Liu, F., Jensen, C.R., Andersen, M.N.: Drought stress effect on carbohydrate concentration in soybean leaves and pods during early reproductive development: its implication in altering pod set. — Field Crops Res. 86: 1–13, 2004.

    Article  CAS  Google Scholar 

  • Ma, L.Y., Wang, L.R., He, S.C., Liu, X., Wang, X.Q.: A new species of Magnolia (Magnoliaceae) from Hubei, China. — Bull. bot. Res. 26: 4–7, 2006a.

    Google Scholar 

  • Ma, L.Y., Wang, L.R., He, S.C., Liu, X., Wang, X.Q.: A new variety of Magnolia (Magnoliaceae) from Hubei, China. — Bull. bot. Res. 26: 517–519, 2006b.

    Google Scholar 

  • Matysik, J., Alia, A., Bhalu, B., Mohanty, P.: Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. — Curr Sci. 82: 525–532, 2002.

    CAS  Google Scholar 

  • Mohapatra, S.S., Poole, R.J., Dhindsa, R.S.: Abscisic acid-regulated gene expression in relation to freezing tolerance of cold-acclimated-specific genes of alfalfa. — Plant Physiol. 89: 375–380, 1988.

    Article  Google Scholar 

  • Mora-Herrera, M.E., Lopez-Delgado, H.A.: Freezing tolerance and antioxidant activity in potato microplants induced by abscisic acid treatment. — Amer. J. Potato Res. 84: 467–475, 2007.

    Article  CAS  Google Scholar 

  • Papageorgiou, G.C., Murata, N.: The unusually strong stabilising effects of glycine betaine on the structure and function of the oxygen-evolving photosystem II complex. — Photosynth. Res. 44: 243–252, 1995.

    Article  CAS  PubMed  Google Scholar 

  • Park, J.H., Oh, S.A., Kim, Y.H., Woo, H.R., Nam, H.G.: Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescenceinducing factors in Arabidopsis. — Plant mol. Biol. 37: 445–454, 1998.

    Article  CAS  PubMed  Google Scholar 

  • Pearce, R.S.: Plant freezing and damage. — Ann Bot. 87: 417–424, 2001.

    Article  CAS  Google Scholar 

  • Rajashekar, C., Lafta, A. Cell-wall changes and cell tension in response to cold acclimation and exogenous abscisic acid in leaves and cell cultures. — Plant Physiol. 111: 605–612, 1996.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Stushnoff, C., Seufferheld, M., Creegan, T.: Oligosaccharides as Endogenous Cryoprotectants in Woody Plants. - Plenum Press, New York 1997.

    Book  Google Scholar 

  • Suojala, T., Lindén, L.: Frost hardiness of Philadelphus and Hydrangea clones during ecodormancy. — Acta agr. scand. B Soil Plant Sci. 47: 58–63, 1997.

    Google Scholar 

  • Teets, T.M., Hummel, R.L., Guy, C.L.: Cold-acclimation of Hibiscus rosa-sinensis L. and Hybiscus syriacus L. in natural and controlled environments. — Plant Cell Environ. 12: 495–502, 1989.

    Article  Google Scholar 

  • Weiser, C.J.: Cold resistance and injury in woody plants. — Science 169: 1269–1278, 1970.

    Article  CAS  PubMed  Google Scholar 

  • Williams, B.J., Pellett, N.E., Klein, R.M.: Phytochrome control of growth cessation and initiation of cold-acclimation in selected woody plants. — Plant Physiol. 50: 262–265, 1972.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zabadal, T.J., Dami, I.E., Goffinet, M.C., Martinson, T.E., Chien, M.L.: Winter injury to grapevines and methods of protection. — Michigan State Univ. Ext. Bull. 2930: 1–101, 2007.

    Google Scholar 

  • Zuther, E., Buchel, K., Hundertmark, M., Sitt, M., Hincha, D.: The role of raffinose in the cold acclimation response of Arabidopsis thaliana. — FEBS Lett. 576: 169–173, 2004.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Forestry Department, Beijing University of Agriculture, Beijing, 102206, P.R. China

    Y. Yang

  2. Laboratory for Silviculture and Conservation, Beijing Forestry University, Beijing, 100083, P.R. China

    N. Yao, Z. K. Jia & L. Y. Ma

  3. National Energy R&D Center for Non-food Biomass, Beijing Forestry University, Beijing, 100083, P.R. China

    J. Duan

  4. Biotechnology Research Center, Three Gorges University, Yichang, Hubei Province, 443002, P.R. China

    F. J. Chen

  5. Forestry Bureau of Wufeng County, Wufeng, Hubei Province, 443400, P.R. China

    Z. Y. Sang

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  1. Y. Yang
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  2. N. Yao
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  3. Z. K. Jia
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  7. L. Y. Ma
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Correspondence to L. Y. Ma.

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Acknowledgement

This study was supported by the Special Fund for Forest Scientific Research in the Public Welfare (201504704), the DABEINONG Research Fund for Young Teacher (15ZK007), the Special Fund of Young Teacher of the Beijing University of Agriculture (SXQN2016107) and the Special Fund of Undergraduate of the Beijing University of Agriculture (BJNXYTW20160412).

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Yang, Y., Yao, N., Jia, Z.K. et al. Effect of exogenous abscisic acid on cold acclimation in two Magnolia species. Biol Plant 60, 555–562 (2016). https://doi.org/10.1007/s10535-016-0623-5

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  • DOI: https://doi.org/10.1007/s10535-016-0623-5

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