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Molecular cloning and expression of four phenylalanine ammonia lyase genes from banana interacting with Fusarium oxysporum

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Biologia Plantarum

Abstract

Phenylalanine ammonia lyase (PAL) is the first enzyme in the phenylpropanoid pathway. In this study, we describe the molecular characteristics of four PAL genes (MaPAL1, MaPAL2, MaPAL3, and MaPAL4) cloned from banana (Musa acuminata L. AAA group, cv. Cavendish) using a rapid amplification of cDNA ends and real time quantitative PCR. The predicted molecular masses of corresponding PALs ranged from 70.2 to 77.3 kDa and their isoelectric points were acidic. At the amino acid level, they shared a high sequence similarity with PALs in the banana DH-Pahang (AA group) genome. Phylogenetic analysis shows that the deduced amino acid sequences of MaPALs had also a high similarity with PALs of other plant species. Expression analysis by semi-quantitative reverse transcriptase PCR reveals that these genes were differentially expressed in various tissues. Enzyme activity of PAL and expression of MaPALs in bananas were induced after infection with Fusarium oxysporum f. sp cubense Tropical Race 4. Our findings suggest that MaPALs play important roles in banana resistance to F. oxysporum.

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Abbreviations

CTAB:

cetyltrimethyl ammonium bromide

DPI:

days post-inoculation

Foc TR4:

Fusarium oxysporum f. sp. cubense Tropical Race 4

GFP:

green fluorescent protein

PAL:

phenylalanine ammonia lyase

qPCR:

quantitative PCR

RACE:

rapid amplification of cDNA ends

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, 571101, P.R. China

    Z. Wang, C.-H. Jia, J.-P. Li, B.-Y. Xu & Z.-Q. Jin

  2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Hainan, 570101, P.R. China

    J.-Y. Li & Z.-Q. Jin

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  1. Z. Wang
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  2. J.-Y. Li
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  3. C.-H. Jia
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  4. J.-P. Li
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  5. B.-Y. Xu
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  6. Z.-Q. Jin
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Correspondence to B.-Y. Xu.

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Acknowledgement

This research was funded by the Ministry of Science and Technology of P.R. China (No. 2011AA10020605) and the Modern Agro-industry Technology Research System (nycytx-33). Z. Wang and J.-Y. Li contributed equally to this work.

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Wang, Z., Li, JY., Jia, CH. et al. Molecular cloning and expression of four phenylalanine ammonia lyase genes from banana interacting with Fusarium oxysporum . Biol Plant 60, 459–468 (2016). https://doi.org/10.1007/s10535-016-0619-1

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  • DOI: https://doi.org/10.1007/s10535-016-0619-1

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