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Molecular characterization and phylogenetic analysis of Wx genes from three Taeniatherum diploid species

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Biologia Plantarum

Abstract

In wheat seeds, starch synthase I or the Waxy protein is an enzyme involved in amylose synthesis. The gene encoding this enzyme is Wx and in this study, eight novel Wx alleles were identified in three diploid Taeniatherum species. The variability of these alleles was evaluated, and their nucleotide sequences were compared with those of homologous alleles from wheat. Two types of Taeniatherum Wx alleles were detected in three diploid species Ta. caput-medusae, Ta. asperum, and Ta. crinitum. A phylogenetic analysis indicates that the Taeniatherum Wx alleles were more closely related to Wx alleles from Aegilops species with C, D, M, and U genomes than to Wx alleles of other species. These alleles represent a potential genetic resource that may be useful in wheat breeding programs.

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Abbreviations

pI:

isoelectric point

MYA:

million years ago

Wx :

waxy

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P.R. China

    S. F. Dai, J. Q. Jiang, Y. N. Jia, X. F. Xue, D. C. Liu, Y. M. Wei, Y. L. Zheng & Z. H. Yan

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  1. S. F. Dai
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  2. J. Q. Jiang
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  3. Y. N. Jia
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  4. X. F. Xue
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  5. D. C. Liu
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  6. Y. M. Wei
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  7. Y. L. Zheng
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  8. Z. H. Yan
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Correspondence to Z. H. Yan.

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Acknowledgement

This research was supported by the National Natural Science Foundation of China (U1403185) and the Key Fund Project of the Sichuan Provincial Department of Education (15ZA0021).

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Dai, S.F., Jiang, J.Q., Jia, Y.N. et al. Molecular characterization and phylogenetic analysis of Wx genes from three Taeniatherum diploid species. Biol Plant 60, 505–512 (2016). https://doi.org/10.1007/s10535-016-0609-3

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  • DOI: https://doi.org/10.1007/s10535-016-0609-3

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