Abstract
Histone deacetylases (HDACs) catalyze histone deacetylation and play an important role in suppression of gene transcription in multiple cellular processes. HDACs are widely distributed in eukaryotes, however, detailed characterrization of HDACs in woody plants is not available. In this study, the sequences of reduced potassium dependency 3/histone deacetylase 1 (RPD3/HDA1) family proteins in black cottonwood (Populus trichocarpa Torr. & A. Gray) were characterized and their expression patterns in response to cold and salt stresses were determined. The RPD3/HDA1 proteins had conserved HDAC domains and can be divided into three classes based on sequence similarity and phylogenetic analysis. The transcripts of the HDAC genes were detected in different amounts in leaves, stems, and roots. The expressions of HDAC genes HDA902, HDA903, HDA904, HDA909, and HDA912 were up-regulated in a cold stress. Interestingly, in a salt stress, most of the HDAC genes were down-regulated. These results indicate that the poplar HDAC genes were regulated by the cold and salt stresses, and the members of the RPD3/HDA1 family play a role in stress responses.
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Abbreviations
- 18S:
-
18S rRNA
- ABA:
-
abscisic acid
- HAT:
-
histone acetyltransferase
- HDA1:
-
histone deacetylase 1
- HDAC:
-
histone deacetylase
- MEGA:
-
molecular evolutionary genetic analysis
- NCBI:
-
National Center for Biotechnology Information
- ORF:
-
open reading frame
- RPD3:
-
reduced potassium dependency 3
- PCR:
-
polymerase chain reaction
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Acknowledgements: This work was supported by a grant from the National Natural Science Foundation of China (31200497), a grant from the Fundamental Research Funds for the Central Universities (2572015CA11) and a grant from the Innovation Project of the State Key Laboratory of Tree Genetics and Breeding (the Northeast Forestry University) (2013B06). The seedlings of Populus trichocarpa were kindly provided by Prof. Yuxiang Cheng from the State Key Laboratory of Tree Genetics and Breeding (the Northeast Forestry University).
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Ma, X.J., Yang, C.P. & Xia, D.A. Characterization and expression analysis of histone deacetylases family RPD3/HDA1 in Populus trichocarpa . Biol Plant 60, 235–243 (2016). https://doi.org/10.1007/s10535-015-0579-x
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DOI: https://doi.org/10.1007/s10535-015-0579-x