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Genome-wide identification and characterization of the DREB transcription factor gene family in mulberry

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The dehydration responsive element binding (DREB) transcription factor (TF) family comprises unique and important proteins involved in abiotic stress responses and tolerance in plants. Although DREB TFs have been well identified and characterized in a few model plants, there is no detailed information available for mulberry. In this study, 110 AP2/ERF family genes were identified based on a genome-wide analysis of the Morus genome database. Among them, 30 Morus notabilis DREB family genes (MnDREBs) were identified. A comparative analysis with DREB gene families from other plants suggests that MnDREBs could be divided into six subgroups (A-1 to A-6) and could have similar functions in response to abiotic stresses since they have similar conserved domains/motifs within each subgroup. The expression patterns of MnDREBs were analyzed using transcriptome data of different organs from M. notabilis and the quantitative real-time polymerase chain reaction. The expression of most MnDREBs was detected in different organs and induced by various abiotic stresses, which suggest their vital roles in abiotic stress tolerance.

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Abbreviations

ABRE:

element involved in the abscisic acid responsiveness

AP2:

APETALA2

ARE:

element for the anaerobic induction

CBF1:

CRT/DRE-binding factor 1

CDS:

corresponding coding sequence

DRE:

dehydration-responsive element

DRTF:

database of rice transcription factors

DREB:

dehydration responsive element binding

ERE:

ethylene-responsive element

ERF:

ethylene responsive element binding factors

GARE:

gibberellin-responsive element

Glu:

glutamine

GWD:

genome-wide duplications

HSE:

element involved in heat stress responsiveness

LTR:

element involved in low-temperature responsiveness

MBS:

MYB binding site

MBSI:MYB:

binding site involved in flavonoid biosynthetic genes regulation

NCBI:

National Center for Biotechnology Information

NJ:

neighbor-joining

NLS:

nuclear localization sequence

ORF:

open reading frame

pI:

isoelectric point

plant TFDB:

plant transcription factor database

RAV:

related to ABI3/VP1

RPL15:

ribosomal protein L15

RT-qPCR:

real-time quantitative polymerase chain reaction

SMART:

simple modular architecture research tool

TAIR:

Arabidopsis information resource

TF:

transcription factor

Val:

valine

WUN:

wound-responsive element

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  1. State Key Laboratory of Silkworm Genome Biology, Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, P.R. China

    X. Q. Liu, J. J. Zhu, C. J. Wei, Q. Guo, C. K. Bian, Z. H. Xiang & A. C. Zhao

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  1. X. Q. Liu
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  2. J. J. Zhu
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  3. C. J. Wei
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  4. Q. Guo
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  6. Z. H. Xiang
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  7. A. C. Zhao
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Correspondence to A. C. Zhao.

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Acknowledgements: This work was supported by grants from the Chongqing Science & Technology Commission (No. cstc2012jjA80039), the China Agriculture Research System (No. CARS-22-ZJ0102), and the National Hi-Tech Research and Development Program of China (No. 2013AA100605-3). The first two authors contributed equally to this paper.

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Liu, X.Q., Zhu, J.J., Wei, C.J. et al. Genome-wide identification and characterization of the DREB transcription factor gene family in mulberry. Biol Plant 59, 253–265 (2015). https://doi.org/10.1007/s10535-015-0498-x

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  • DOI: https://doi.org/10.1007/s10535-015-0498-x

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