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Characterization and expression pattern analysis of microRNAs in wheat under drought stress

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Plant microRNAs (miRNAs) play important roles in regulating plant growth, development, and responses to abiotic stresses. In this study, 38 miRNAs (TaMIRs) from wheat (Triticum aestivum L.), 36 from the miRBase database, and two from our previous work were characterized and subjected to an expression pattern analysis under normal conditions and a drought stress. A semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time quantitative PCR (qPCR), and small RNA blot analyses revealed that two TaMIRs (TaMIR1120 and TaMIR1123) were root-predominant and two TaMIRs (TaMIR1121 and TaMIR1134) were leaf-predominant. Seven TaMIR precursors showed altered expressions after the drought; of these, TaMIR1136 was upregulated, whereas TaMIR156, TaMIR408, TaMIR1119, TaMIR1129, TaMIR1133, and TaMIR1139 were downregulated. These seven drought-responsive TaMIRs showed dose-dependent and typical temporal expression patterns during drought induction, and they gradually returned back under the normal growth conditions. The drought-responsive and the tissue-predominant TaMIRs had varying numbers of target genes. Randomly selected target genes exhibited opposite expression patterns to their corresponding TaMIRs suggesting that they were regulated by distinct TaMIRs through a post-transcriptional cleavage. The target genes regulated by drought-responsive and tissue-predominant TaMIRs are involved in various cellular processes, such as signal transduction, transcriptional regulation, primary and secondary metabolisms, development, and defense responses. These results provide a novel insight into the miRNA-mediated responses of wheat to drought stress.

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Abbreviations

ABA:

abscisic acid

AFB3 :

auxin receptor 3 gene

CSD :

cytosolic Cu/Zn superoxide dismutase genes

DRE:

dehydration-responsive element

EST:

expressed sequence tag

MS:

Murashige and Skoog

PEG:

polyethylene glycol

Pi:

inorganic phosphate

qPCR:

real-time quantitative PCR

RISC:

RNA-induced silencing complex

RT-PCR:

reverse transcriptase polymerase chain reaction

TaMIR:

Triticum aestivum microRNA

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Authors and Affiliations

  1. College of Agronomy, Agricultural University of Hebei, Baoding, 071001, P.R. China

    Y. -Y. Zhao, C. -J. Guo, W. -W. Duan, C. -Y. Ma, H. -M. Chan & K. Xiao

  2. College of Life Sciences, Agricultural University of Hebei, Baoding, 071001, P.R. China

    X. -J. Li, Y. -L. Wen & W. -J. Lu

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  1. Y. -Y. Zhao
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  2. C. -J. Guo
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  3. X. -J. Li
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  9. K. Xiao
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Correspondence to K. Xiao.

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Acknowledgments: We would like to thank two anonymous reviewers and the editors whose detailed comments and careful work helped to improve the manuscript. This work was financially supported by the National Science Foundation of China (No. 31371618, 31201674), the Natural Science Foundation of Hebei (C2013204094) and the Key Laboratory of Crop Growth Regulation of Hebei Province.

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Zhao, Y.Y., Guo, C.J., Li, X.J. et al. Characterization and expression pattern analysis of microRNAs in wheat under drought stress. Biol Plant 59, 37–46 (2015). https://doi.org/10.1007/s10535-014-0463-0

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