Skip to main content
SpringerLink
Log in

Characterization of RAM to SAM transitions in Selaginella microphylla grown in vitro

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

In vitro morphogenetic ability of plant cells has been demonstrated in diverse species of angiosperms and gymnosperms but no such report is available in the genus Selaginella till date. We have established an in vitro morphogenic root culture where indole butyric acid (IBA) induced profuse branched and unbranched roots in Selaginella microphylla. We observed inter-convertibility of root apical meristem (RAM) to shoot apical meristem (SAM) in presence of IBA and showed that intact roots are also capable of transformation. Friable callus was obtained from roots on prolonged (∼50 weeks) root cultures. By isolating total RNA from each of the developmental stages, we performed cDNA synthesis followed by random amplification, sequencing, and BLAST analysis of differentially expressed transcripts to correlate morphological events with the changes on molecular level. The results revealed sequence matches to genes involved in diverse cellular processes such as transcription, translation, photosynthesis, replication, secondary metabolism, stress response, and plant defense suggesting ancient origins of such proteins and the evolutionary conservation of biological function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

IBA:

indole butyric acid

NCBI:

National Centre for Biotechnology Information

PCR:

polymerase chain reaction

RAM:

root apical meristem

RAPD:

randomly amplified polymorphic DNA

SAM:

shoot apical meristem

References

  • Agarwal, M., Shrivastava, N., Padh, H.: Advances in molecular marker techniques and their application in plant sciences. — Plant Cell Rep. 27: 617–631, 2008.

    Article  PubMed  CAS  Google Scholar 

  • Banks, J.A.: Selaginella and 400 million years of separation. — Annu. Rev. Plant Biol. 60: 223–238, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Bao, Y., Dharmawardhana, P., Mockler, T.C., Strauss, S.H.: Genome scale transcriptome analysis of shoot organogenesis in Populus. — BMC Plant Biol. 9: 132, 2009.

    Article  PubMed  Google Scholar 

  • Boehm, M., Romero, E., Reiseinger, V., Komenda, J., Yu, J., Eichacker, L.A., Dekker, J.P., Nixon, P.J.: Investigating the early stages of photosystem II assembly in Synechocystis sp.PCC6803: isolation of CP47 and CP43 complexes. — J biol. Chem. 286: 14812–14819, 2011.

    Article  PubMed  CAS  Google Scholar 

  • Bricker, T.M., Lowrance, J., Sutton, H., Frankel, L.K.: Alterations of the oxygen-evolving apparatus in a (448)Arg-(448)S mutant in the CP47 protein of photosystem II under normal and low chloride conditions. — Biochemistry 40: 11483–11489, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Che, P., Lall, S., Nettleton, D., Howell, S.H.: Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture. — Plant Physiol. 141: 620–637, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Chen, L., Song, Y., Li, S., Zhang, L., Zou, C., Yu, D.: The role of WRKY transcription factors in plant abiotic stresses. — Biochim. biophys. Acta 1819: 120–128, 2011.

    PubMed  Google Scholar 

  • Chen, Y.H., Tsai, Y. J., Huang, J.Z., Chen, F.C.: Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture. — Cell Res. 15: 639–657, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Citterio, S., Piatti, S., Albertini, E., Aina, R., Varotto, S., Barcaccia, G.: Alfalfa Mob1-like proteins are involved in cell proliferation and are localized in the cell division plane during cytokinesis. — Exp. cell. Res. 312:1050–1064, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Dobrenel, T., Marchive, C., Sormani, R., Moreau, M., Mozzo, M., Montane, M.H., Menand, B., Robaglia, C., Meyer, C.: Regulation of plant growth and metabolism by TOR kinase. — Biochem. Soc. Trans. 39: 477–481, 2011.

    Article  PubMed  CAS  Google Scholar 

  • George, E.F., Hall, M.A., De Klerk, G.-J. (ed.): Plant Propagation by Tissue Culture. Volume 1. The Background. 3rd Ed. — Springer, Dordrecht 2008.

    Google Scholar 

  • Grasser, M., Kane, C.M., Merkle, T., Melzer, M., Emmersen, J., Grasser, K.D.: Transcript elongation factor TFIIS is involved in Arabidopsis seed dormancy. — J. mol. Biol. 386: 598–611, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Jha, T.B., Mukherjee, S., Basak, A., Adhikari, J.: In vitro morphogenesis in Selaginella microphylla (Kunth.) Spring. — Plant Biotechnol. Rep. DOI 10.1007/s11816-012-0255-y 2012.

    Google Scholar 

  • Jiang, K., Feldman, L.J.: Regulation of root apical meristem development. — Annu. Rev. cell. dev. Biol. 21: 485–509, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Kalkhoven, E., Teunissen, H., Houweling, A., Verrijzer, P.C., Zantema, A.: The PHD type zinc finger is an integral part of the CBP acetyltransferase domain. — Mol. cell. Biol. 22: 1961–1970, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Lijsebettens, M.V., Montagu, M.V.: Historical perspectives on plant developmental biology. — Int. J. dev. Biol. 49: 453–465, 2005.

    Article  PubMed  Google Scholar 

  • Liu, Y., Bassham, D.C.: TOR is a negative regulator of autophagy in A. thaliana. — PLOS One 5: e11883, 2010.

    Article  PubMed  Google Scholar 

  • Markmann-Mulisch, U., Subramanian, A.R.: Nucleotide sequence and linkage map position of the genes for ribosomal proteins L14 and S8 in the maize chloroplast genome. — Eur. J. Biochem. 170: 507–514, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Mortensen, S.A., Sønderkær, M., Lynggaard, C., Grasser, M., Nielsen, K.L., Grasser, K.D.: Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS. — FEBS Lett. 585: 1929–1933, 2011.

    Article  PubMed  CAS  Google Scholar 

  • Newton, A.L., Sharpe, B.K., Kwan, A., Mackay, J.P., Crossley, M.: The transactivation domain within cysteine/histidine-rich region 1 of CBP comprises two novel zinc-binding modules. — J. biol. Chem. 275: 15128–15134, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Nimbalkar, S.B., Harsulkar, A.M., Giri, A.P., Sainani, M.N., Franceschi, V., Gupta, V.S.: Differentially expressed gene transcripts in roots of resistant and susceptible chickpea plant (Cicer arietinum L.) upon Fusarium oxysporum infection. — Physiol. mol. Plant. Pathol. 68: 176–188, 2006.

    Article  CAS  Google Scholar 

  • Ohl, S., Hedrick, S.A., Chory, J., Lamb, C.J.: Functional properties of a phenylalanine ammonia lyase promoter from Arabidopsis. — Plant Cell 2: 837–848, 1990.

    PubMed  CAS  Google Scholar 

  • Rushton, P.J., Somssich, I.E., Ringler, P., Shen, Q.J.: WRKY transcription factors. — Trends Plant Sci. 15: 247–258, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Stahl, Y., Simon, R.: Plant primary meristems: shared functions and regulatory mechanisms. — Curr. Opin. Plant Biol. 13: 53–58, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Stahl, Y., Wink, R.H., Ingram, G.C., Simon, R.: A signaling module controlling the stem cell niche in Arabidopsis root meristems. — Curr. Biol. 19: 909–914, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Tanaka, M., Wakasugi, T., Sugita, M., Shinozaki, K., Sugiura, M.: Genes for the eight ribosomal proteins are clustered on the chloroplast genome of tobacco (Nicotiana tabacum): similarity to the S10 and spc operons of E.coli.— Proc. nat. Acad. Sci. USA 83: 6030–6034, 1986.

    Article  PubMed  CAS  Google Scholar 

  • Wochok, Z.S., Sussex, I.M.: Redetermination of cultured root tips to leafy shoots in Selaginella willdenovii. — Plant Sci. Lett. 6: 185–192, 1976.

    Article  Google Scholar 

  • Zhang, Y., Chen, M., Chou, B.B., Jermiin, L.S., Larkum, A.W.: Evolution of the inner light harvesting antenna protein family of cyanobacteria, algae and plants. — J. mol. Evol. 64: 321–331, 2007.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, Presidency University, 86/1 College Street, Kolkata-73, West Bengal, India

    T. B. Jha

  2. Department of Biotechnology, Presidency University, 86/1 College Street, Kolkata-73, West Bengal, India

    S. Bandyopadhyay

  3. Department of Genetics, University of Calcutta, 35 B.C. Road, Kolkata-19, West Bengal, India

    K. Nandagopal

Authors
  1. S. Bandyopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Nandagopal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. B. Jha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. B. Jha.

Additional information

Acknowledgements: The work was supported by the Department of Biotechnology Research Associateship programme (DBT-RA), Govt. of India.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bandyopadhyay, S., Nandagopal, K. & Jha, T.B. Characterization of RAM to SAM transitions in Selaginella microphylla grown in vitro . Biol Plant 57, 597–600 (2013). https://doi.org/10.1007/s10535-013-0325-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-013-0325-1

Additional key words

Search

Navigation